We narrowed to 214 results for: TIR
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TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...
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Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....Check Out Fluorescent Protein Biosensors Browse the Entire Fluorescent Protein Collection ... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m.... the antisense orientation (Figure 1, top). The entire DNA cassette would be flanked by two pairs of target... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m.... has recently been hitting the headlines, with sotires like the potential devastation of the palm oil ... -
The Materials Science of Optogenetics Experiments
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m....the ferrule implant must be maintained during the entire protocol, otherwise optical stimulation will be... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....be dull and painful to sit through (imagine the entire audience playing Candy Crush or reading email).... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....still beats lentivirus. In mice, AAV is almost entirely non-immunogenic — meaning when conducting in vivo... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m..... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....exponentially replicate the target DNA of interest. This entire reaction used to be done manually in water baths... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....the antibodies were able to immunoprecipitate the entire complex in three different cell lines. The Gräslund... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....PEG solution. PEG takes several hours on a heated stir plate to dissolve and requires monitoring so that... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....depending on the cell line so old cells should be retired! Conversely, cells younger than the 3rd – 4th passage... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....gone up to a poster where the presenter spends the entire time talking to the poster, as opposed to engaging... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m.... a plasmid whose copy number is unknown, it is entirely possible that you are working with a low copy ... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m.... Furthermore, point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m...., the drive could eventually spread through the entire wild population with unpredictable ecological effects... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....the same mRNA. The term "self-cleaving" is not entirely accurate, as these peptides are thought to function... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work...