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We narrowed to 216 results for: TIR

Showing: 141 - 160 of 216 results
  1. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... order. (It’s also why you can’t skip a module entirely, but instead will have to replace it with a spacer...
  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... the antisense orientation (Figure 1, top). The entire DNA cassette would be flanked by two pairs of target...
  3. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...Super-resolution imaging of actin filaments with mGold2. Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused...
  4. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...still beats lentivirus. In mice, AAV is almost entirely non-immunogenic — meaning when conducting in vivo...
  5. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...
  6. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...the antibodies were able to immunoprecipitate the entire complex in three different cell lines. The Gräslund...
  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...depending on the cell line so old cells should be retired! Conversely, cells younger than the 3rd – 4th passage...
  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... a plasmid whose copy number is unknown, it is entirely possible that you are working with a low copy ...
  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...
  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...  Furthermore, point-by-point scanning along the entire specimen makes imaging too slow and unwieldy for...
Showing: 141 - 160 of 216 results