Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 628 results for: UTY

Showing: 141 - 160 of 628 results

  1. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...in pull-down assays: Epitope tagging provides a solution Proteins - the workhorses of the cell – never ...expensive, and not to mention frustrating. One elegant solution to sidestep the antibody problem is to tag the...tagging. Using our 96-format pipeline, we can routinely achieve 5-30% tag knock-in efficiency across different...experiments using the csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow...should be pursued, however, clonal lines must be cautiously analyzed for any damage (indels) to the promoter...

  2. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...promoter regulating the gene of interest with a constitutive, robust M24 promoter. Originally isolated from...from the Mirabilis mosaic virus, the constitutively active M24 promoter has been modified with duplicated...large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is functional...antibiotic resistance gene is controlled by a weaker constitutive Nos promoter, and it can be used to select both...

  3. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...legal approvals so the plasmids are ready for distribution as soon as the paper is published, while giving...with permission to release their plasmids for distribution, and we’ve found that this step was sometimes...already moved on to their next position. We do routinely review deposits that are left in the hold status... published we’ll get the plasmids online for distribution instead of risking leaving them on hold indefinitely...

  4. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... shy away from looking beyond its confines. The beauty of serendipity often emerges at the intersections... key to unlocking new perspectives or even revolutionizing your research. Why serendipity matters Serendipity...lead to unexpected discoveries and innovative solutions. Allowing for serendipity in your lab research...benefits by encouraging activities beyond the lab routine. It encourages you to think beyond the confines...PhDs in their careers with actionable steps and execution plans.    More resources on the Addgene blog ...

  5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology...Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off simply ...sustained activation period is beneficial for use in therapeutic or behavioral applications where drawn out effects...

  6. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... a team to get a lot of stuff done.  Factors contributing to job satisfaction Being a good manager is ...organization and feel the importance of their contributions. Flexible work schedule Flexibility to manage...personal growth do not exist or if they feel underutilized in their present positions By removing the...will address more specific tactics managers can utilize to be in touch with the needs of the team and to...

  7. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues from The Scintillon...Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition of the enhanced GFP... to FRET and stochastic single-molecule super resolution imaging. mNeonGreen also has little sequence ...Addgene! References Ai, Hui-wang, et al. "Directed evolution of a monomeric, bright and photostable version...

  8. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...to complete your story: an introduction, and a resolution.   Fig. 2: The structure of a story - ... left out, which can be a big challenge!  The resolution Throughout your conflict, you’re encouraging ...audience - via the scientific method - to the resolution, or the ending of the story.  At this point you...complex story, and then build on that in your resolution to really dig into the significance of the results...

  9. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...communication graduates are often well-equipped. Many institutions are sincerely interested in connecting with ...of how to appeal to a given audience and how to utilize a wide array of media in doing so—can prove invaluable...risk perception, scientific uncertainty and precautionary regulation was all necessary to inform the experimental...unsettled science. Understanding just how the public utilizes insights gleaned from scientific research is, ...

  10. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...deftly coordinated by the eukaryotic cell for the execution of many complex cellular behaviors, such as differentiation...engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex ...off switch The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

  11. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...commonly used ones for transfers between academic institutions.  What is the UBMTA, you ask? Well, organizations... agreement. The One-Time Plasmid Deposit and Distribution Agreement establishes the terms under which ... researchers additionally have the option of distributing under Addgene’s Industry MTA (IMTA), which allows...depositing institute that decides the terms of distribution for their plasmids. MTAs at Addgene Since Addgene...

  12. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...a single person. With thousands of scientists routinely using and thinking about FPs, it would be great... designed with the goal of encouraging user contributions (using simple web forms) while also maintaining...and we very much appreciate even those small contributions). Of course, no community-editable database ... include incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum...

  13. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and...processes we use to ensure high-quality library distribution. You can also use these tips as a starting point... scaled to 1, to create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the ...gain a sense of the uniformity of the library distribution, with an AUC of 0.5 representing perfect uniformity...

  14. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...treatment options, as well as non-pharmacological solutions to modify the course of this condition are urgently...glowing bacteria and a fish robot to detect water pollution Dr. Julio Ernesto Valdivia Silva, Universidad ...related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology resources...Biology Resources Learn about Global Plasmid Distribution ...

  15. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...did To measure the pH of our samples, we used a solution of cell culture medium, which is buffered and ...room temperature and used the color of the liquid solution and pH paper to measure the pH. We compared the...note that the change in pH only occurred when the solution thawed, as the CO2 was confined to the tube headspace...et al., 2013). However, the pH changes in our solutions are transient and return to normal once the tube...

  16. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...(accession numbers, reagent details, author contributions, etc.) before their paper can be published. ...do. All of that helps, but it’s only a partial solution.  How do we get where we want to be? The part ... in science needs both a job and funding, so institutions and funders, to a large extent, hold the keys...across life and physical science, and editorial contribution to the strategic development of Cell Press. ...

  17. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...developed in terms of the collection, storage, and distribution of the plasmids and their ability to identify...plasmids so scientists aren’t bogged down with distributing them and spending more time on research. What...plan manage risk. My job and my team’s job is to routinely assess the efficiency and effectiveness of the...auditor (CISA), and certified health insurance executive (CHIE). He is a member of both the Internal Institute...

  18. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...alternative such as a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic.... At a very low volumetric density and with a resolution of less than 100 µm, the ARG reporters could ...group at the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes...

  19. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...problem/task/situation, come up with potential solutions, and decide on a course of action that effectively...by paying attention to how your mentors reach solutions and ask them to explain their logic. Or you can... probably did not just automatically come to a solution. Consider the thoughts that went through your ... challenge yourself to think of more creative solutions. Ultimately, problem solving is less about technical...

  20. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...labs have recently published work that provides a solution to this problem. A miniature CRISPR-Cas system...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...mismatch repair (MMR) interfered with the desired resolution of prime editing intermediates. Building upon...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...
Showing: 141 - 160 of 628 results