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We narrowed to 168 results for: acr.2

Showing: 141 - 160 of 168 results

  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...graduate student leaders in science communication from across the US and Canada convene at ComSciCon's annual...

  2. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...create a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within...likely that this amino acid sequence will be shared across other proteins. Conversely, polyclonal antibodies...

  3. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...account when simulating a digest as depicted in Lane 2. Controlling methylation Finally, you can control...Learn to Troubleshoot Your Sequencing Results Use REPLACR Mutagenesis to Mutate Your Plasmids Resources ...

  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...developed several methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation...

  5. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... those that also infect the liver.    Fig. 2: Summary of the generational evolution of the AAV ...rate of less than 0.3%, both on and off target. Across all loci tested, error rates overall ranged from...

  6. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig...adenoviruses are known to cause respiratory disease across species, as well as cancer in hamsters, although...

  7. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...PubMed PMID: 8491198. PubMed Central PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human...Finally, viral delivery is typically straightforward across applications, while electroporation of a human,...

  8. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...communicating in science brings stronger networking across laboratory and disciplinary boundaries and can ...

  9. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for.... coli cell, but vectors can be widely utilized across many different cell types and each one requires...

  10. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include...times with the same samples and compare the results across runs. If the results vary considerably, then the...protein level of a target that is thought to be equal across all samples, such as a housekeeping protein (GAPDH...tubulin). A loading control confirms equal loading across samples and equal protein transfer from the gel...

  11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...this year. Designing the donor plasmid Figure 2: Donor plasmid design strategy. A donor plasmid contains...components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor plasmid into the cells ...

  12. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors are...will need to be more assertive to get your point across. Some supervisors might try to intimidate you with...

  13. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...communicate the nuances of protein structure in a flat 2-D images. In the early days of structural biology,...large ribosomal subunit, he kept sliding his palm across the surface of the protein where the nascent polypeptide...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...Fluorescent Proteins: Multiplexing and Optogenetics across Scales. Trends Biotechnol 36, 1230-1243 (2018)....near-infrared fluorescent proteins for multicolor imaging across scales. Nat Commun 11, 239 (2020). Oliinyk, O.S...

  15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...) 12 (>100) xCas9(3.7)-ABE 69 (1.4) 43 (2) 21 (3) Not examined 16 (>100) Average percent...off-target activity. Surprisingly, off-target effects across 5 gRNAs with NGG PAM sequences were actually lower...

  16. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...PMID: 25538079. PubMed Central PMCID: PMC4353587. 2. Stephen, Andrew G., et al. "Dragging ras back in ... in the kit, the transcript most commonly found across all TCGA tumor samples is represented, making the...

  17. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ... replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through insertional...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most...

  18. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...that numbers are not switched around or misread.  2. Track the container - It is just as important to ...weeks in transit and some have been transported across oceans and continents. Each deposit may consist...

  19. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...passion  Skills such as research, collaboration across multiple disciplines, writing, project management...
Showing: 141 - 160 of 168 results