We narrowed to 176 results for: acr.2

...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...coordinates optical stimulation of its associated fibers across multiple channels and can also record tissue temperature...

...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...require when making such a decision remains spread across literature in publications that introduce these...

...start selecting and testing systemic capsids! Figure 2 contains a decision tree for selecting a capsid, while... highlighted below the table.     Figure 2: This decision tree can help guide your choice on ...transfer to the adult brain. Nature Biotechnology, 34(2), 204–209. https://doi.org/10.1038/nbt.3440 Goertsen...capsids, when writing this post, we didn’t come across a broadly used working definition of systemic capsids...expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested in C57BL/6J...brain cells, with a higher efficiency (82 — 85% across the brain in mice) and greater specificity than...effectively and predominantly transduces neurons across brain regions, with reduced off-target expression...

...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...X1.1 efficiently transduce brain endothelial cells across diverse mice strains and rats. Figure from Chen... et al. (2023). Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific...

...integrates into the trnS-leu2 locus.   Figure 2: Introducing Mobile-CRISPRi into bacteria. Top: A ...constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to 150-fold... reduction, with a median knockdown of ~40-fold across all species measured. Beyond understanding the ...

... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...secreted from producer cells also contain cellular macromolecules (e.g. proteins, nucleic acids) which possibly...

...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...refresher if you ever lose track of which acronym is which. Acronym Full name Notes   Base transition ...close at hand? Once again, this post has a lot of acronyms, so we've included a glossary at the end of this...polymerase known to preferentially incorporate A bases across from abasic sites. This approach resulted in a ... case, encouraging it to incorporate a new base across from the abasic site before filling the empty space...

...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...graduate student leaders in science communication from across the US and Canada convene at ComSciCon's annual...

...create a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within...likely that this amino acid sequence will be shared across other proteins. Conversely, polyclonal antibodies...

...account when simulating a digest as depicted in Lane 2. Controlling methylation Finally, you can control...Learn to Troubleshoot Your Sequencing Results Use REPLACR Mutagenesis to Mutate Your Plasmids Resources ...

...Nutr, 129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...developed several methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation...

...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig...adenoviruses are known to cause respiratory disease across species, as well as cancer in hamsters, although...

... those that also infect the liver.    Fig. 2: Summary of the generational evolution of the AAV ...rate of less than 0.3%, both on and off target. Across all loci tested, error rates overall ranged from...

...PubMed PMID: 8491198. PubMed Central PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human...Finally, viral delivery is typically straightforward across applications, while electroporation of a human,...

... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...communicating in science brings stronger networking across laboratory and disciplinary boundaries and can ...

...and are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for.... coli cell, but vectors can be widely utilized across many different cell types and each one requires...

...as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include...times with the same samples and compare the results across runs. If the results vary considerably, then the...protein level of a target that is thought to be equal across all samples, such as a housekeeping protein (GAPDH...tubulin). A loading control confirms equal loading across samples and equal protein transfer from the gel...

...this year. Designing the donor plasmid Figure 2: Donor plasmid design strategy. A donor plasmid contains...components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex) and the donor plasmid into the cells ...

...out for help so you can get the support you need. 2) Prepare an agenda for every meeting Supervisors are...will need to be more assertive to get your point across. Some supervisors might try to intimidate you with...

...communicate the nuances of protein structure in a flat 2-D images. In the early days of structural biology,...large ribosomal subunit, he kept sliding his palm across the surface of the protein where the nascent polypeptide...