We narrowed to 231 results for: bon
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TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...
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Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....lentivirus. Also, note that due to the size of the backbone, transformations require electroporation. References... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....disease. “My mentors Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base editor... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....students PCR, they first had to understand hydrogen bonds and then build up from there. In every project, ... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....targets. Due to their size and lack of disulfide bonds, monobodies can be easily expressed in transfected... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....various delivery methods, including plasmid, ribonucleoprotein, and AAV. Read the paper in Nature Chemical... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....cysteines, such as those that make up the disulfide bonds between antibody chains. 3) Endogenous carbohydrates... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have enough context... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....try to use an excess of insert compared to the backbone plasmid; a starting point could be a molar ratio... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....promoters were also tested in different plasmid backbones that varied in size or carried different genes... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog PostPublishedApril 12, 2016, 2:30 p.m....CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m....disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....things like butanol - very simple molecules - a few carbons and oxygens. Nothing too much more sophisticated...