We narrowed to 313 results for: epor
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TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity...
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Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....interest with an additional protein module that can report on location. This approach enables the study of...9759496. 3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m.... where two proteins are fused to complementary reporter proteins that can only assemble when in close ... ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m.... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m.... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m.... self-labeling tools: Tetracysteine: The first reported chemical labeling tag, from Roger Tsien’s lab ...collection. CLIP-tag: Also in 2008, Kai Johnsson’s lab reported another AGT-based tag named CLIP-tag, which reacts... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....piggyBac plasmids Tol2 Tol2 was the first reported active DNA transposon in vertebrates. It was discovered...mutations. In these screens, the transposons encode reporter genes, mutagenic cassettes, or barcodes. When ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m.... unbiased methods, high background BLESS Not reported Cell-based (in vitro) Can be used on tissue from...senstive to time since cell fixing HTGTS Not reported Cell-based Identifies translocations Limited by... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....transgenic mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically...using 2-photon microscopy as this hasn't been well reported for many fluorescent proteins. Additional fluorescent... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m.... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...therapeutic targets in acute myeloid leukemia." Cell reports 17.4 (2016): 1193-1205. PubMed PMID: 27760321. ... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m....plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog PostPublishedDec. 20, 2017, 2:33 p.m....working under in the lab or has won, awards, media reports, and membership in groups related to the foreign...: You the individual must be named in the media report (NPR, New York Times, respected online sources)... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog PostPublishedNov. 3, 2015, 3:30 p.m....no longer available), is a simple catalogue of reported sgRNA designs. The second database, CrisprGE (...experiments and use the results to re-evaluate all tools. Report your findings to help everyone else. CRISPR technology... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog PostPublishedSept. 3, 2020, 12:15 p.m....system, Ye’s lab could edit a tdTomato fluorescent reporter gene in mice using Minicircle DNA vectors encoding...seen in the figure below. Next, by using a dual reporter fluorescence assay, Hemphill et al. demonstrated... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog PostPublishedDec. 5, 2023, 2:15 p.m....Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors... -
8 Great Depositor Research Stories
TypeBlog PostPublishedDec. 15, 2022, 2:15 p.m....introduced us to RUBY-red, a bright red pigment reporter for plants. Cas7-11 and PASTE: Omar Abudayyeh... -
DIY DNA Ladders from Penn State University
TypeBlog PostPublishedJuly 14, 2017, 2:30 p.m....Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog PostPublishedOct. 5, 2017, 1:13 p.m....learning from peers, and being receptive to those who report to you. Chapter 5: The types of science that Crystal... -
New Viral Vectors - Spring 2025
TypeBlog PostPublishedApril 29, 2025, 1:30 p.m....help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...