We narrowed to 214 results for: gnal

1. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...between the lowest and highest level of “output” signal, was only about 10-fold, precluding the study of...

2. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ...PMC1526508. 2. H. Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila...

3. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   Blog Post

   Published

   July 6, 2017, 1:59 p.m.

   ...Opto-CRAC which allows a researcher to control immune signaling and inflammatory responses remotely with spatiotemporal...

4. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...molecule. A higher brightness increases the detected signal and therefore high brightness is an important feature...

5. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ...endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process the array into functional...

6. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...individually assessed for a positive or negative (digital) signal at the end of the reaction (Figure 1). Partitions...

7. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...investigate the effects of activating particular signaling pathways in specific cell types (typically neurons...

8. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...complex to chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, ...

9. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx...

10. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ...The researchers validated their work with the “signalome,” a 12-plex mixed-subclone system using mStrawberry...

11. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...can recruit multiple frankenbodies to amplify the signal.  Liu et al. Genes Cells. 2021. https://doi.org...

12. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    Published

    June 29, 2021, 1:15 p.m.

    ...ligand is bound, then you’ll get a false negative signal - the antibody can’t recognize the protein even...

13. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ...contains a promoter, the gene of interest, and a polyA signal. Created with biorender.com. ITR structure  WT ...

14. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...They accomplish this through nuclear localization signals which can traffic their viral particles inside ...

15. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...transparent and rigorous studies will give false signals from time to time. False positives in original ...

16. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    Published

    June 27, 2019, 12:25 p.m.

    ...translation of the reporter gene resulting in GFP signal. With the addition of standards, the assay is semi-quantitative...

17. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...bright, exhibits good localization, has high signal:noise ratio, high sensitivity, responds quickly, is...

18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...increased expression of transforming growth factor-β signaling, possibly inducing the secretion of growth promoting...

19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...targeted to the cytosol when expressed without a signal sequence and to the mitochondria via citrate synthase...

20. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...dynamics throughout the cell cycle. To optimize the signal to noise ratio of the system, they modified GFP-dCas9...