We narrowed to 216 results for: gnal

1. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

   Type

   Blog Post

   Published

   July 27, 2017, 1:11 p.m.

   ...work with photoswitchable components of the ERK signaling pathway in our recent blog post. Fatima Enam: ...

2. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...between the lowest and highest level of “output” signal, was only about 10-fold, precluding the study of...

3. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   Published

   Oct. 19, 2023, 1:15 p.m.

   ...contains a promoter, the gene of interest, and a polyA signal. Created with biorender.com. ITR structure  WT ...

4. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   Published

   March 21, 2023, 1:15 p.m.

   ...They accomplish this through nuclear localization signals which can traffic their viral particles inside ...

5. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...The researchers validated their work with the “signalome,” a 12-plex mixed-subclone system using mStrawberry...

6. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...can recruit multiple frankenbodies to amplify the signal.  Liu et al. Genes Cells. 2021. https://doi.org...

7. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ...ligand is bound, then you’ll get a false negative signal - the antibody can’t recognize the protein even...

8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

   Published

   July 6, 2017, 1:59 p.m.

   ...Opto-CRAC which allows a researcher to control immune signaling and inflammatory responses remotely with spatiotemporal...

9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...molecule. A higher brightness increases the detected signal and therefore high brightness is an important feature...

10. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ...endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process the array into functional...

11. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...individually assessed for a positive or negative (digital) signal at the end of the reaction (Figure 1). Partitions...

12. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...investigate the effects of activating particular signaling pathways in specific cell types (typically neurons...

13. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...complex to chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, ...

14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx...

15. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...transparent and rigorous studies will give false signals from time to time. False positives in original ...

16. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...PMC1526508. 2. H. Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila...

17. Click Biology: How to Click and Play to Explore Biology

    Type

    Blog Post

    Published

    Oct. 21, 2025, 1:15 p.m.

    ...cellular behavior like degradation or electrophile signaling. Many of these tools can be found in Addgene's...

18. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ... extremely small at 12-15 kD but can have weak signals due to their monovalent nature. They are stable...

19. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...receptor. However, in some cases the fluorescent signal may be critical for the experiment. Since the fused...

20. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type

    Blog Post

    Published

    April 13, 2017, 2:30 p.m.

    ...targeted to the cytosol when expressed without a signal sequence and to the mitochondria via citrate synthase...