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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 250 results for: hal.2

Showing: 141 - 160 of 250 results

  1. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end) - Panel Alex Tucker (Ginkgo Bioworks)...their curation of citable, linkable figures. Cause #2: Hidden “negative” data We all want to be able to ...Pamela Hines (Senior Editor at Science) Edward J. Hall (Professor of Philosophy at Harvard University) ...experiments they’re reading about weren’t changed halfway through in order to chase a positive result. Beyond...

  2. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources on the Addgene blog CRISPR101: Validating... cancer immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-... in a developing or developed immune system was lethal (LaFleur et al., 2024).   Figure 1: Schematic...

  3. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...cycle of one step. Challenges in the LEGO science video process One major challenge when we started was...will bring people together and make these daily challenges a little easier to overcome. We want to encourage...

  5. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...short-read sequencing are highlighted in Figure 2. Figure 2: Overview of short-read sequencing technology... up the pace Our QC team constantly faces the challenge of keeping up with the large amount of QC analysis...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...’re delivering their payload to.   Figure 2: Numbers of AAV tools, by category, available from... substrates, light, or engineered ligands (Figure 2). Biosensors, which include calcium and voltage sensors...depending on their serotype, are particularly challenging. Their efficacy in any given system can be affected...

  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...sequence is unique compared to the rest of the genome.  2) The target is present immediately upstream of a Protospacer...utilized allowing for PAM flexibility. Fig. 2: Prime editor engaging target DNA. The reverse transcriptase...essential genes whose knockout would cause cell lethality. The Krüppel associated box (KRAB) is a naturally...

  9. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision...practical terms, what GreenGate can do is “save you half a year,” in comparison to more tedious and time-...

  10. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...First, the light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide and...and CIB1 – which they borrowed from Arabidopsis thaliana. The results are light-inducible transcriptional...

  11. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ... both substantial merit and national importance; (2) The foreign national must be well positioned to advance...outstanding achievements in your specific academic field, (2) that you have three 3 years of research experience..., Casseus Law suggests a mix of experts including 2 dependents –people who have worked with you i.e. your...for companies to submit an application on their behalf.  If an individual obtains a cap subject H-1B (...

  12. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...similar optical quality (magnification of 140X and 2 micron resolution). I have worked many hours in a ...University). Right there on the floor outside the lecture hall. It was wonderful to see the ad-hoc ‘sciencing’ ...

  13. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...much has been done with CRISPR technology in just 2 years and excitement about how much more there is ...systems, modifications to the RNA components, and challenging target genomes, such as the malaria-causing Plasmodium...Addgene depositor, discussed some of the many challenges of creating biological circuits, and I can only...

  15. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...would label everything but the CD8+ T cells (Figure 2). Even better, we can use the same fluorophore for...gating on the negative population.     Figure 2: Dump gating labels all unwanted cell lineages with...capacity and speed of cells. (a) With each division half of the dye molecules are lost and the fluorescence...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...cells will be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic... Ononiwu, F., Pal, D., Manikas, J., Taveras, C., Hall, N. A., Silva, J. D., Freshour, J., & Hehnly, H....

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...antibody selectivity. Additionally, it can be a real challenge to troubleshoot RNAi failures. Potential explanations...cell survival or proliferation, an additional challenge is that only partial or ephemeral knockdown will...confirmation of antibody selectivity even more challenging. Correlation with RNA or Proteomic data from ...supportive of their specificity for the protein. A challenge to this approach is that the identity of the epitope...the specific sample and protocol. This can be a challenge to confirm. As such, the approach used may need...

  18. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes...SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. ...screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications..., is composed of only 529 amino acids, less than half the size of either Cas9 or Cas12a. Despite its small...

  19. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547 Kunda, Z. (...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...a phenomenon called “motivated reasoning,” the catchall term for a range of neat little tricks our minds...

  20. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...and trophies are awarded and coveted. I’ve only won 2 trophies over the years, which is either an indication...different labs to chat informally about their challenges, successes, and expertise. Who knows what could...
Showing: 141 - 160 of 250 results