We narrowed to 330 results for: iCre

...resist the temptation to “just do it myself” or micromanage –you don’t have time for that and you won’t be...best way. This facilitates success and trust. Micromanaging is bad for everyone. Document action plans...

...work in his or her lab of choice? Is the group micromanaged or does it thrive in an off hands environment...labs tend to be more "chaotic" and offer less micromanaging, they typically offer more flexibility in choosing...

... Vectors. Microorganisms, 12(2), 310. https://doi.org/10.3390/microorganisms1202031038399714. PMID: 38399714...

...can use GECIs to watch neurons fire under the microscope. To add another tool to our toolbox, our team... compatible with the lasers available in your microscope setup. Figure 1: Excitation (dotted) ...

...plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert genes in a cleavage...a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et al, 2022.  Find...

...reaction. For instance, sodium azide, a common antimicrobial agent added to buffers, can bind to amine groups... of 0.01% w/v can be used as an alternative antimicrobial.  Conjugates And HRP brings us handily to the...

...DNA transformation system.Unlike most other microorganisms, yeast have both a stable haploid and diploid...are considered “high copy”. A fragment from the 2 micron circle (a natural yeast plasmid) allows for 50+...

...localization accuracy when used in super-resolution microscopy by reducing the distance between the target and...antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org...

...the cell,” says Pedre Pérez. Figure 2: Micrographs of the biosensor expressed in the human cell ...understand mouse cortical and hippocampal neural microcircuits. “In the longer term, this screening platform...

...Surprisingly our observations at the electron microscope showed us that, in monkey neurons, the large ...interest the membrane.   Figure 1: Electron microscope images showing ultrastructural localization of...

... making sure that what we observe through the microscope represents reality as accurately as possible....double-strand break repair pathway, relying instead on microhomology-mediated end-joining (MMEJ). To find out more...

... is effective to target short ncRNAs, such as microRNAs that are around 20 base pairs long. Using a single...indels induced by CRISPR/Cas9 in the 5’ region of microRNA lead to its depletion and Drosha processing retardance...

...the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd generation ...titer of lentiviral particles than the original lentiCRISPR v1. Special note from the Zhang lab: We are constantly...

...operon podcast segment With the help of engineered microbes, used coffee grounds could be economically repurposed...transfer), and FLIM (fluorescence lifetime imaging microscopy) assays. ShadowG is darker than other dark YFP...

...absorbance of each well, which is measured using a microplate reader, is directly proportional to the amount... et al., 2015.   The maximum OD that most microplate readers can detect is 4.0, so if you max out ...

...specific site. To generate a transgenic mouse, microinjection of DNA or infection with a viral vector containing...the site of DNA damage. These nucleases are microinjected into a zygote, which is then implanted into ...

...help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table top device ...has a roller to go over caps and push them into micronic tubes instead of pushing them in one by one. It...

...CRISPR-Cas systems. 5. Nat Rev Microbiol. 2015 Sep 28. doi: 10.1038/nrmicro3569. PubMed PMID: 26411297 Resources...

...types, who never have time to mentor you, and Micromanagers, who question every detail of your project and...know-it all experts, wet-blanket negativists, and micromanagers), while other supervisors might be nice people...

...solution is then melted (and thereby sterilized) by microwaving and brought into the hood. A 48-well plate is...fused with a fluorescent protein), fluorescence microscopy can theoretically be used to select specific ...