We narrowed to 229 results for: mel

1. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...within tissues before cell lysis. These products, namely RNAlater®  from Thermo and RNAProtect® from Qiagen...

2. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ....2021.02.007 Moroz-Omori, E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally...

3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ...networks. “Developmental bioelectricity is an extremely exciting emerging field, with many implications...

4. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   Published

   April 20, 2023, 4 p.m.

   ...nervous and freeze, or forget a point, or talk extremely quickly. You realize, much too late, that you ...

5. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...antibodies, nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are ...

6. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ...graduate students accepted in STEM fields (6). Melanie Sinche is a STEM career counselor, Director of ...

7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...anywhere else, but for synthetic circuits, they are extremely orthogonal. It’s a different perspective and you...

8. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   Published

   Nov. 14, 2024, 2:15 p.m.

   ... ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying and assessing ncRNA...

9. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...   Figure 1: (Top) Schematic overview and timeline of the Golden Gate (GG) cloning approach. Individual...

10. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...to solutions. It is also volatile and toxic (and smells bad!), so be sure to use it under a fume hood. ...

11. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox...

12. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The Cas Hybrid...

13. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ...MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed evolution of adenine base editors...

14. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...epitopes are available; this type of ELISA is extremely specific and sensitive to the target protein due...

15. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ... Melina Fan...

16. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...combinations. Even with current technologies, it can be extremely hard to predict how a single TU, or even an individual...

17. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...Mouravlev A, Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D,...

18. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...M.K. Montgomery, S.A. Kostas, S.E. Driver, C.C. Mello. Potent and specific genetic interference by double-stranded...

19. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ... The first genetically encoded FRET biosensor, Cameleon (Miyawaki et al., 1997), was designed to measure...

20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ..., X. laevis (frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae ...