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We narrowed to 484 results for: multi

Showing: 141 - 160 of 484 results

  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...cloning required. As with other model systems, multiple gRNAs can be expressed to modify several genomic...genomic loci at once (get more information on multiplexing gRNAs here). Cas9 is commonly tagged with a nuclear...Zhang Y, Qi Y (2015) A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation...Resources on the Addgene Blog Read Up on CRISPR Multiplexing Tools Validate Your Genome Edit Design Your ...

  2. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...hotspot for dystrophin, exons 45-55, there are multiple common deletions that maintain the protein’s reading...skipping? For such a therapy to be successful, multiple requirements must be met. First, CRISPR would ...dystrophy. gRNAs can be designed to flank a single or multiple exons that, when deleted through NHEJ, will restore...Thakore PI, Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction...

  3. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ..., you’ll run the same protein concentration in multiple lanes and cut up the membrane to treat each lane...are a little trickier than gels and may require multiple runs to optimize or troubleshoot.   Before you...protein gradient, compare your diluted samples. If multiple dilutions are showing the same signal (Figure ...also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal from the...antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly lysed Different...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ... extended to multiplexed probe sets that allow the simultaneous interrogation of multiple potential SNP...extends earlier studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used...: PMC148763. 15. Pingle, Maneesh R., et al. "Multiplexed identification of blood-borne bacterial pathogens...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...inverted repeats (Figure 1). With this technique, multiple genes can be simultaneously silenced if a consensus...sequence is used (~90% identity between targets) or multiple target sequences are concatenated. hpRNA has been... robustly self-organize at the cellular and multicellular scale.     References Bezanilla M, Perroud P...J-F (2018) Engineering Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen...

  6. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells ...

  7. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...

  8. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ... – the dreaded ‘undruggable’ proteins – having multiple hits or an entire pathway of possibilities greatly... complex diseases, it can aid in generation of multiple edits or implementation of expression systems ...candidates are selected.      CRISPR aids in multiple stages of the drug discovery pipeline.   ...

  9. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...genome engineering tool offers flexibility, multiplexibility, and ease of use. In order for this technology...the tool’s specificity, orthogonality, and multiplexibility in various species and describe an assortment...from experts in the field, and enabling access to multiple plasmids used for various platform applications...

  10. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...rate, conventional CRISPR screens require a low multiplicity of infection (MOI) during cell library construction...in high quality data even when used at a high multiplicity of infection, because this setup allows for ...allowed tracing of the performance of each gRNA multiple times within the same experiment by counting both...

  11. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat protein binding...constant tracking. You can then directly monitor multiple rounds of translation from this single mRNA; you...cases, creating a reporter mRNA requires inserting multiple hairpin sequences into the coding sequence and...

  12. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal loci...plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR labeling of chromosomal loci in human ...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...only having one binding site per protein. Having multiple antibodies allows you to use them in parallel ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...  Multiple Authors...

  14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ... enzymes, some of which are also functional in multiple species (read Arbor Biotechnologies' press release...nt direct repeat) mediating potent knockdown. Multiplexing from the CRISPR array yielded >90% knockdown... necessary. Konermann et al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference...

  16. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ... cell research has prompted the development of multiple virus packaging methods. Across these methods,...sequence. This post was written and updated by multiple Addgenies. It was originally published in 2018...  Multiple Authors...

  17. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...autofluorescence and phototoxicity and even allowing multiple proteins to be visualized simultaneously. Complementation... with incompatible substrates can also allow multiplexing with experiments that supply both substrates... Multiple Authors...

  18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...deeply connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine...spread and drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes...proliferate and cannot undergo apoptosis, thus multiplying uncontrollably and building up tumors. “Friendly...

  19. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest case we have a ...insert are correct. In other cases, we deal with multiple inserts or internal mutations that require more... DNA-editing software to perform alignments of multiple sequences to visually check for homology and mismatches...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...barcodes infect the target cell population at low multiplicity of infection (MOI) such that each individual...erlotinib. Looking at barcode abundances among multiple replicate populations, they found that similar...went on to show that combination treatment with multiple drugs reveals even smaller preexisting populations...
Showing: 141 - 160 of 484 results