We narrowed to 481 results for: multi
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TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m...., you’ll run the same protein concentration in multiple lanes and cut up the membrane to treat each lane...are a little trickier than gels and may require multiple runs to optimize or troubleshoot. Before you...protein gradient, compare your diluted samples. If multiple dilutions are showing the same signal (Figure ...also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal from the...antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly lysed Different...
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When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m.... extended to multiplexed probe sets that allow the simultaneous interrogation of multiple potential SNP...extends earlier studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used...: PMC148763. 15. Pingle, Maneesh R., et al. "Multiplexed identification of blood-borne bacterial pathogens... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....inverted repeats (Figure 1). With this technique, multiple genes can be simultaneously silenced if a consensus...sequence is used (~90% identity between targets) or multiple target sequences are concatenated. hpRNA has been... robustly self-organize at the cellular and multicellular scale. References Bezanilla M, Perroud P...J-F (2018) Engineering Artificial MicroRNAs for Multiplex Gene Silencing and Simplified Transgenic Screen... -
8 Great Depositor Research Stories
TypeBlog PostPublishedDec. 15, 2022, 2:15 p.m....us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...editing tool for large inserts. DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells ... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m.... – the dreaded ‘undruggable’ proteins – having multiple hits or an entire pathway of possibilities greatly... complex diseases, it can aid in generation of multiple edits or implementation of expression systems ...candidates are selected. CRISPR aids in multiple stages of the drug discovery pipeline. ... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....genome engineering tool offers flexibility, multiplexibility, and ease of use. In order for this technology...the tool’s specificity, orthogonality, and multiplexibility in various species and describe an assortment...from experts in the field, and enabling access to multiple plasmids used for various platform applications... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....rate, conventional CRISPR screens require a low multiplicity of infection (MOI) during cell library construction...in high quality data even when used at a high multiplicity of infection, because this setup allows for ...allowed tracing of the performance of each gRNA multiple times within the same experiment by counting both... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....only having one binding site per protein. Having multiple antibodies allows you to use them in parallel ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:... Multiple Authors... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m....Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal loci...plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR labeling of chromosomal loci in human ...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat protein binding...constant tracking. You can then directly monitor multiple rounds of translation from this single mRNA; you...cases, creating a reporter mRNA requires inserting multiple hairpin sequences into the coding sequence and... -
Fluorescent Proteins 101: Luciferases
TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....autofluorescence and phototoxicity and even allowing multiple proteins to be visualized simultaneously. Complementation... with incompatible substrates can also allow multiplexing with experiments that supply both substrates... Multiple Authors... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m.... cell research has prompted the development of multiple virus packaging methods. Across these methods,...sequence. This post was written and updated by multiple Addgenies. It was originally published in 2018... Multiple Authors... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....you need more than one? Multiplex assays Multiplex assays involve multiple antibodies, each with a different... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m.... enzymes, some of which are also functional in multiple species (read Arbor Biotechnologies' press release...nt direct repeat) mediating potent knockdown. Multiplexing from the CRISPR array yielded >90% knockdown... necessary. Konermann et al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....deeply connected to these 3 stages and contains multiple cell types: fibroblasts, myofibroblasts, neuroendocrine...spread and drug resistance? Although we’ve seen how multiple types of cells affect tumor progression, enzymes...proliferate and cannot undergo apoptosis, thus multiplying uncontrollably and building up tumors. “Friendly... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest case we have a ...insert are correct. In other cases, we deal with multiple inserts or internal mutations that require more... DNA-editing software to perform alignments of multiple sequences to visually check for homology and mismatches... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....barcodes infect the target cell population at low multiplicity of infection (MOI) such that each individual...erlotinib. Looking at barcode abundances among multiple replicate populations, they found that similar...went on to show that combination treatment with multiple drugs reveals even smaller preexisting populations... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m.... of time that you want to probe your blot with multiple antibodies. You’ll want to set up your blot with...stripping methods. If you are planning to detect multiple proteins through several rounds of stripping and...clearing the way for the second blot. There are multiple ways to strip blots, and the best choice will ... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...widely successful in primates. The authors used a multispecies approach to generate and screen a library of... Multiple Authors...