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Showing: 141 - 160 of 317 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... concentrations of iodixanol to separate out contaminants from an impure AAV preparation. 15%, 25%, 40%...R.A., & Samulski, R.J. (2007). Production of recombinant adeno-associated viral vectors for in vitro and...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...sure that the sample is pure and free of any contaminants that could affect the measurements. You'll need...Addgene's Ready-to-Use Antibody collection Addgene's Recombinant Antibody Plasmids collection  ...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...into this mixture to absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids...placing the dipstick into a wash solution removes contaminants while nucleic acids stick around. Finally, purified...

  5. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively...at Stanford University. She studies cell fate maintenance in the stomatal lineage and has made countless...

  6. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...fluorescent biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...

  7. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal...different antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...-2 research   By Ashley Waldron Ready-to-use recombinant antibodies were recently added to the Addgene...Image from Addgene. These antibodies are recombinant versions of mouse monoclonal antibodies generated...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Kalamegam, G., Ahmed, F., Abbas, M., Sait, K., Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., ...Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies...

  11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele...generating human somatic cell gene knockouts using recombinant adeno‐associated viruses." Nucleic acids research...

  12. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either produced de novo using...an expanding collection of readily accessible recombinant antibodies and plasmids. To encourage collaboration...

  13. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... can hydrolyze phosphatidylcholine (PC), the predominant membrane lipid, to phosphatidic acid (PA) or ...Plasmids References Aljiboury, A. A., Ingram, E., Krishnan, N., Ononiwu, F., Pal, D., Manikas, J., Taveras...

  15. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...glnV44 λ- JM109   General cloning and plasmid maintenance, blue/white screening, partly restriction-deficient...(mrr-hsdRMS-mcrBC) Pir1    For cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant...

  16. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...

  17. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired chimeric...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

  18. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...

  19. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...from multiple primer pairs, one can infer the predominant state of registers within a sample using a metric...states in a sample; instead, it will suggest the predominant state of registers in a sample based on a similarity...

  20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral...
Showing: 141 - 160 of 317 results