Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 363 results for: nsf

Showing: 141 - 160 of 363 results

  1. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...control. A good positive control is bacteria transformed with the same backbone plasmid. If this control...strain. A good negative control strain is an untransformed culture of the same strain of bacteria you used...Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive Clones with Us ...

  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...interactions. In later years, improvements to yeast transformation protocols also helped establish the yeast two-hybrid...applied to the bait in a single population (e.g. transforming both fusion plasmids, or mating two single haploid... haploid yeast fusion plasmid transformants into a diploid strain for screening), or arrayed on a 96 well...

  3. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...indels after DNA repair. B) M. abscessus is first transformed with Cas9 on an integrative plasmid containing...resistance selection marker KanR. This strain is then transformed with the sgRNA cassette on a plasmid expressing...

  4. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...sequencing or restriction digest! Then, you can transform or transfect the cells that you want to use for your... reactions are performed, the next step is to transform competent E. coli cells and select the positive...

  6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...constitutively expressed to identify successfully transfected cells. C-to-T editing by the APOBEC-Cas9 editosome... to cells segregated solely by a reporter of transfection, which only report the efficiency of plasmid...frequency of base edits of interest than solely transfected cells. “Gene On” (GO)- a functional reporter ...

  7. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...errors like uneven pipetting or irregular membrane transfer. Figure 1: A loading control is a type .... No pipetter is perfect, and proteins may not transfer precisely evenly across the entire blot. So how... in the loading control are due to loading and transfer errors — not to any factors from before the proteins...

  8. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...Measuring local temperature variations and heat transfer at the subcellular level requires a probe with...fast measurement of temperature and local heat transfer. Image reproduced from Lu et al. 2022, under CC-BY-NC-ND...plasmids here!   Lu K, et al. Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...writers have been curious about the capability of transforming the genetic code of living organisms and its...that Streptococcus pneumoniae bacteria could be transformed from one organism to the other. This is now famed...THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES. The Journal of Experimental...

  10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments possible in a wide...CRISPR technology into C. elegans has been a transformative innovation for our community. Developments ... genomic locus (CRISPR-ChAP-MS) could further transform the range of experiments imaginable in C. elegans...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Nakahara, K. (2011). Local and retrograde gene transfer into primate neuronal pathways via adeno-associated... H. (1979). Selective retrograde transsynaptic transfer of a protein, tetanus toxin, subsequent to its..., H.G., and Strick, P.L. (1989). Transneuronal transfer of herpes virus from peripheral nerves to cortex...

  12. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback...

  13. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...popular envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus background...px or 1080 px).         Do you do a lot of transformations with the plasmids you receive from Addgene?...

  14. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR...

  15. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    .... Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection by Alyssa Cecchetelli...

  16. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ... the snail, the larvae reach the eyestalk and transform them into a pulsating display imitating a moving...grasshoppers and crickets. When it’s ready to transform into an aquatic adult, it forces its insect host...

  19. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...product is introduced into the cell by direct transformation. A given insert will typically contain both...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...

  20. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...releases energy as a photon. The photon energy is transferred to the acceptor fluorophore, a circularly permuted...known to enhance gene expression) were then cotransfected with these constructs, providing the machinery... principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase...
Showing: 141 - 160 of 363 results