We narrowed to 230 results for: pET

1. Visualizing Genomic Loci with CRISPR-Sirius

   Type

   Blog Post

   Published

   April 18, 2019, 12:53 p.m.

   ...this system out on chromosome 19 by targeting 46 repetitive loci that contained  ≥20 copies of the gRNA target...

2. Summer SciComm Series: A PhD in Science Communication

   Type

   Blog Post

   Published

   Aug. 23, 2022, 1:15 p.m.

   ...9587-9592. doi:10.1073/pnas.1704882114 Kahan, D., Peters, E., Wittlin, M., Slovic, P., Ouellette, L. L.,...

3. DAP arrays for multiplex gene editing

   Type

   Blog Post

   Published

   July 7, 2022, 1:15 p.m.

   ...plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided, different tRNAs...

4. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

   Type

   Blog Post

   Published

   June 1, 2021, 1:15 p.m.

   ... power of collaboration, which despite the competitiveness of cutting-edge research, is still one of the...

5. Creating accessible biology activities in schools with BioBits

   Type

   Blog Post

   Published

   Aug. 2, 2018, 12:56 p.m.

   ...skills like developing a hypothesis, precisions pipetting, and good record keeping. Finally, if you are ...

6. K. phaffii: Rising to the Occasion

   Type

   Blog Post

   Published

   April 4, 2023, 1 p.m.

   ... in the general research community. Phillips Petroleum Company first commercially developed K. phaffii...

7. Hot Plasmids - January 2023

   Type

   Blog Post

   Published

   Jan. 31, 2023, 2:15 p.m.

   ... affect FPs during these processes. The Liu and Petsko labs recently engineered hyperfolder YFP (hfYFP...

8. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   Published

   May 28, 2020, 1:15 p.m.

   ... Maya Peters Kostman...

9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes required...

10. GCE4All: Making Genetic Code Expansion Accessible

    Type

    Blog Post

    Published

    June 15, 2023, 1:15 p.m.

    ...biology scene in 1989 through a seminal paper from Peter Schultz’s lab describing a technique that allowed...

11. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...nature, extra safety measures, like replication incompetent viral particles, are required. We will be covering...

12. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ... Medal in the Undergrad category at the iGEM Competition 2019. He has an active interest in scientific...

13. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ... and help each other practice meeting people – compete for fastest to set up a coffee date or most cards...

14. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...//doi.org/10.1242/bio.059310 Fraser N, Mayr P, Peters I (2021) Motivations, concerns and selection biases...

15. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...used along other thiol-containing acceptors in competition assays. “I believe that improved biosensors or...

16. To Each HIS Own

    Type

    Blog Post

    Published

    March 2, 2023, 2:15 p.m.

    ...then eluted using a high imidazole buffer that outcompetes the interaction between the protein of interest...

17. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...(GAL4) was supplied. In addition to labeling repetitive telomeric sequences, Chen et al. successfully...

18. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...primers that bind within or must read through repetitive regions (such as LTRs or ITRs), hairpins, or ...

19. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    Published

    Feb. 11, 2014, 12:13 p.m.

    ...assume that their advisor alone applies for these competitive grant applications; however, in some cases, your...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...