We narrowed to 317 results for: pho;
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TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m.... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby The ease of observing ...
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Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....scientists access to a wide array of promoters and fluorophores for validating and testing purposes. We will...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559 For more specific procedural...in marine biology and minoring in studio arts photography. References and resources References Silhavy... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....these TFs to see if the differentiated cells had morphologies consistent with the predicted cell type (e.g...neuron predicted differentiation), and 15/17 morphologically aligned. Documentation of the network that ... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....sensing protein YFI. When light is absent, YFI phosphorylates FixJ, which binds to the FixK2 promoter to ... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....tumorigenesis assays, drug epistasis assays, and morphological profiling can be used as generalizable strategies... are described in this paper Systematic morphological profiling of human gene and allele function ... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....example of how this works. Make sure to order 5’ phosphorylated oligos or treat with T4 PNK. Delivery options... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog PostPublishedJune 6, 2019, 12:41 p.m....transmission within minutes. Constructs using the photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin...gas-filled protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins ... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m...., a large set of headphones can act as a Do Not Disturb sign, even if the headphones aren’t actually playing... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...cascade. This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....and cells. They can be used either with or as fluorophores in a wide variety of colors. To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of bi-stable anion... medicine, evolution of body plans, synthetic morphology, and even artificial intelligence," says Michael... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....costs low. Most will work with you remotely (by phone, Skype or Google Hangout) but in-person coaching...White Consulting frequently do sessions by Skype or phone. Samantha Sutton is another very experienced and... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....actions of the whole complex. For example, the phosphorylation status of SRC-3 defines its association with... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m....frequent CDG type characterized by deficient phosphomannomutase (PMM) activity due to mutations in the PMM2... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....contact information, and potentially a photograph of yourself. A photo can be very useful in the event that... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones). Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...