We narrowed to 325 results for: pho;

1. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   Blog Post

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   July 18, 2017, 12:41 p.m.

   ...affordable biosensors that detect herbicides, such as glyphosate, in water. He aims to leverage a glycine oxidase...oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction’s metabolites. One useful...

2. An Addgene Summer Internship

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   Blog Post

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   Aug. 8, 2024, 7:22 p.m.

   ...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

3. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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   Blog Post

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   Nov. 1, 2017, 2:04 p.m.

   ... to students, like how to pour a gel [for] electrophoresis or doing a dissection video. I did that in ...themselves. “You open up the box and there’s a gel electrophoresis tray in there. Or the dissection specimens....

4. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   Blog Post

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   July 16, 2019, 12:59 p.m.

   ...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

5. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   Blog Post

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   June 27, 2018, 1:16 p.m.

   ...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...

6. RUBY-Red Siliques

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   Blog Post

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   March 1, 2022, 2:15 p.m.

   ... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby.   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby. The ease of observing...

7. Antibodies 101: Chimeric Antibodies

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   Blog Post

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   April 25, 2023, 1:15 p.m.

   ...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...

8. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

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   Aug. 2, 2022, 1:15 p.m.

   ...Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559 For more specific procedural...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...

9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   Blog Post

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   March 30, 2023, 1:15 p.m.

   ...these TFs to see if the differentiated cells had morphologies consistent with the predicted cell type (e.g...neuron predicted differentiation), and 15/17 morphologically aligned. Documentation of the network that ...

10. New and Upcoming Viral Vectors - September 2019

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    Blog Post

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    Sept. 12, 2019, 1 p.m.

    ...scientists access to a wide array of promoters and fluorophores for validating and testing purposes. We will...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...

11. Sweating the Small Stuff: Details in the Lab

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    Blog Post

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    Feb. 8, 2022, 2:15 p.m.

    ..., a large set of headphones can act as a Do Not Disturb sign, even if the headphones aren’t actually playing...

12. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

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    May 11, 2017, 2:30 p.m.

    ...tumorigenesis assays, drug epistasis assays, and morphological profiling can be used as generalizable strategies... are described in this paper Systematic morphological profiling of human gene and allele function ...

13. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

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    May 9, 2017, 2:12 p.m.

    ...example of how this works. Make sure to order 5’ phosphorylated oligos or treat with T4 PNK. Delivery options...

14. Antibodies 101: Flow Cytometry Controls

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    Blog Post

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    June 6, 2024, 1:50 p.m.

    ...should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

15. Plasmids 101: Inducible Promoters

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    Blog Post

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    Jan. 18, 2018, 2:34 p.m.

    ...sensing protein YFI. When light is absent, YFI phosphorylates FixJ, which binds to the FixK2 promoter to ...

16. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

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    Nov. 19, 2019, 2:08 p.m.

    ...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...cascade. This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene...

17. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

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    June 6, 2019, 12:41 p.m.

    ...transmission within minutes. Constructs using the photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin...gas-filled protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins ...

18. Antibodies 101: Affinity Reagents

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    Blog Post

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    April 14, 2022, 1:15 p.m.

    ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

19. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

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    June 15, 2017, 2:30 p.m.

    ...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...

20. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

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    Oct. 29, 2019, 2:52 p.m.

    ...inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of bi-stable anion... medicine, evolution of body plans, synthetic morphology, and even artificial intelligence," says Michael...