Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 359 results for: plasmids 101

Showing: 141 - 160 of 359 results

  1. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ... Synthetic BiologyAuthor: Niko McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author: Lila ...Marjorie Buss and Mengtong "Tom" DuanVisualizing Your Plasmid Request Data Author: Joachim Goedhart  Created ...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj; ...

  2. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral Applications...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (...Molecular Biology, 79(1), 163–183. https://doi.org/10.1016/0022-2836(73)90277-5. PubMed PMID: 4355596 King...virus. Virology, 46(3), 866–876. https://doi.org/10.1016/0042-6822(71)90087-0. PubMed PMID: 4332980...

  4. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...and other promoter systems, head over to our Plasmids 101: Inducible Promoters article.) Modifying AraC.... pBLADEONLY: This plasmid contains only BLADE and is based on the pTRC99a plasmid. It does not include...Molecular Biology Reference Download Addgene's Plasmids 101 eBook ...reporter downstream of the PBAD promoter. To use this plasmid with your gene of interest, you would replace mCherry...PBAD promoter. If you already have a pBAD33-based plasmid expressing your gene of interest, all you have ... and shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the...nuclear export system (LEXY) here. Optogenetic plasmids from the Khammash lab can also be found here. ...

  5. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...blog Plasmids 101: Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: Golden...through your plasmid stocks. To avoid cross-contamination, we recommend retrieving plasmids from your original...Calculation for the plasmid volume for MoClo experiments. The term ‘insert’ refers to plasmids carrying each...will find some useful tips to help you make your plasmid construction with MoClo a walk in the park.  Before...promoter, a coding sequence, a terminator, or other plasmid part? Do you want to fuse your protein to any tags...restriction enzymes, and it will create the resulting plasmid map and identify any mismatches that are preventing...Level 0, Level 1, and Level 2 assemblies using the plasmid sequence files provided with every Addgene MoClo...

  7. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction...

  8. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...blog:  Plasmid-Based Recombinant Antibodies  Plasmids 101: Secondary Nanobody Toolbox Antibodies 101: Introduction...important collection.  Creating the Plasmids To begin constructing the plasmids for expression of mAbs, the Trimmer...expression plasmids were DNA sequenced to determine the VH and VL sequence. These plasmids have been deposited...scientific community with the plasmid sequences available from each plasmid webpage. Importantly, the methods...NABOR are recombinant antibodies expressed from plasmids that have been deposited with Addgene. Here, we... first antibodies distributed by Addgene.  The plasmids used to generate these antibodies were based off... to create an amplicon that  was cloned into a plasmid backbone for expression of the mAb heavy and light...

  9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...special collections page Download the Plasmid 101 eBook See all plasmids deposited by the Voigt lab ... gene expression and plasmid number. In a suite of plasmids modified from pSC101 with copy numbers ranging...this case, plasmid copy number is the input and gene expression is the output. The input, plasmid copy number...stationary phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, ...RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid by altering...genome or a plasmid. Thus, a stabilized promoter can allow a system to be moved from a plasmid to the genome...PMC3163176. Resources on the Addgene Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology...

  10. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...CRISPR blog posts Find CRISPR Plasmids at Addgene  ... crispr 101...Genome Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources at Addgene: Read our...

  11. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...affect nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop...addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources More resources on the Addgene...

  12. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors CRISPR 101: Cytosine Transversion Editors...CRISPR plasmids for base editing Get a broad overview with our CRISPR Guide Check out our CRISPR 101 ebook...Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016/j.molcel.2024.01.021 Tong, H., Liu, N., Wei, Y....

  13. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... — including the recently released Viral Vectors 101 2nd edition — I was incredibly excited when we picked...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...

  14. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources on Addgene.org Browse the Fluorescent...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

  15. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... the PEDAR plasmids   Additional resources on the Addgene blog Download the CRISPR 101 eBook Find ...guide Find CRISPR plasmids by function Find recently deposited plasmids Topics: CRISPR, Other CRISPR Tools... where we highlight a few of the newest CRISPR plasmids available at Addgene. If you want to read more...will cover: Miniature CRISPR-Cas systems SEND plasmids Prime editing improvements Miniature CRISPR-Cas...Read the paper in Molecular Cell Find the CasMINI plasmids Efficient CRISPR editing with a hypercompact ...paper in Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome editing by a miniature CRISPR-Cas12f...cells using various delivery methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime ...CRISPR 101: Cas9 Structural Biology Additional resources on Addgene.org Browse CRISPR plasmids for base...overview with our CRISPR Guide Check out our CRISPR 101 ebook ...

  17. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... https://doi.org/10.1016/j.xgen.2024.100519 More resources from Addgene Plasmids 101: Modular Cloning ...restriction digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just ...correctly banded clones for plasmid sequencing and found 98% of the plasmids were perfectly correct. Because... Mammalian Plasmid pRDA_791 (contains only orio and ampR) Drosophilia Plasmid pRDB_051 (for...the assembled plasmid. It shows you each component, with the ID of the component plasmid, component name...the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating...Cloning Addgene's CRISPR Guide Plasmids 101: Golden Gate Cloning...

  18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...Vectors 101: An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101: Systemic...Request Browse all in-stock AAV preps Browse all AAV plasmids .... Cell, S0092-8674(25)00692-0. https://doi.org/10.1016/j.cell.2025.06.026 Halbert, C. L., Allen, J. M....Neurons. Neuron, 92(2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021 Walters, R. W., Yi, S. M.,...276(23), 20610–20616. https://doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006... Gene Therapy, 14(3), 316–327. https://doi.org/10.1016/j.ymthe.2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna...

  19. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Addgene blog Viral Vectors 101: AAV Variables That Matter Viral Vectors 101: An Introduction to AAV References...about that collection. Table 1: Systemic capsid plasmids available from Addgene Capsid Published ...re interested in. Then request your capsid (or plasmids) and let the real work begin — validation, validation...dose between 1 x 1011 and 5 x 1011 vg per animal (i.e., 10 and 50 µL of vector at 1 x 1013 vg/mL titer), ...AAV-PHP.S is typically used at a dose between 3 x 1011 and 1 x 1012 vg*. Most systemic capsids are easy to produce...produce in high titer, but higher doses (>1 x 1012 vg total in mice) may increase the chances of inducing... At lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB can transduce a greater number...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...Addgene Blog: Plasmids 101: How to Verify Your Plasmid Plasmids 101: An Inside Look at NGS Plasmid Quality ...results of the plasmids in our repository with known reference sequences, such as full plasmid sequences provided...up their plasmid and cloning verification steps and free up more time for using your plasmids instead!...Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid...provided by the laboratories that deposit their plasmids with us or other entries in NCBI’s numerous databases... grown over the years (we now have over 60,000 plasmids!), the number of sequencing results we analyze...busy week, we may need to analyze more than 200 plasmids as part of our quality control process. Consequently...
Showing: 141 - 160 of 359 results