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We narrowed to 904 results for: CAN

Showing: 791 - 800 of 904 results
  1. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... and the requirements of these different systems can vary (for example, xCas9 is a variant with increased...CRISPR, It's time to start thinking about how you can used them in your experiments. Use the following ...possible loci. Without binding to the guide RNA, Cas9 cannot cut. 2. Bind to target DNA in the presence of a...
  2. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...reduce the undesired C→G or C→A conversions that can happen with BE3s. These byproducts likely resulted...efforts have also yielded a panel of Tad-CBEs that can precisely target single cytosines in the base editing...even at sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells...other editing strategies are there? CBEs and ABEs can induce 4 out of 12 possible base-to-base changes....2020). There are also RNA base editors, which you can learn more about in this blog post: CRISPR 101: RNA...sequence preferences, evoAPOBEC1-BE4max, which is significantly more effective at editing cytosines preceded...mixed population of edits, ABEs do not display significant A to non-G conversion at target loci. The removal...
  3. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...photosensitizers and a quick zap of the proper color light, you can do just that.  Photosensitizing dyes and proteins...complex complex and the MG-2I-dL5** fluorogen that can be used to ablate cells in culture and in vivo.  ...
  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ... are trickier to identify because their contents can be at various stages of degradation. Additionally...GFP-tagged version of LC3, the mammalian Atg8p homolog, can also be used to monitor autophagy, but under some...
Showing: 791 - 800 of 904 results