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We narrowed to 892 results for: CAN

Showing: 791 - 800 of 892 results

  2. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...out, especially since videos on synthetic biology can be difficult to find. I hope that many people will...

  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...with a specificity far greater than the one that can be achieved using pharmacological or electrophysiological...on the other side, are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch ...opsin expression in specific neuronal cells? This can be achieved using Cre-dependent viruses, such as ...subsequently photo-stimulated through fiberoptic cannulas directly implanted in the injection site and connected...

  4. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...each target has a unique species or isotype that can be probed with a distinctly conjugated secondary ...antibody. Consider too that some secondary antibodies can cross react with other species. For example, some...

  5. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...editing at frequencies comparable to SpCas9. SpRY can also edit NYN PAMs, but with less efficiency and ...will have a PAM located next to it. The SpRY Cas9 can also work with NYN PAMs; it just isn’t always ideal...compatibility. This alternative PAM requirement adds a significant amount of flexibility compared to a strict single-compatibility...increases the flexibility of gRNA placement significantly over SpCas9 and more so than Cas9s with an NG...

  7. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...our “Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies... order well in advance whenever possible, as we cannot expedite the application process.  More information...

  8. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...answer any questions, and get feedback on how we can be more helpful to our community.  You'll also find...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...reduce the undesired C→G or C→A conversions that can happen with BE3s. These byproducts likely resulted...even at sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells...other editing strategies are there? CBEs and ABEs can induce 4 out of 12 possible base-to-base changes....2020). There are also RNA base editors, which you can learn more about in this blog post: CRISPR 101: RNA...sequence preferences, evoAPOBEC1-BE4max, which is significantly more effective at editing cytosines preceded...mixed population of edits, ABEs do not display significant A to non-G conversion at target loci. The removal...the development of near-PAMless Cas9 variants significantly expands the library of accessible genomic targets...
Showing: 791 - 800 of 892 results