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We narrowed to 892 results for: CAN

Showing: 801 - 810 of 892 results

  2. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...our new curated Microbiology Collection page, you can browse for plasmids by species and find our guides...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be...

  3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...lines.   dCas9-VP64 Description CRISPR activation can occur through fusing dCas9 with VP64, a strong transcriptional...sequences, causing targeted gene regulation. This can be used to activate transcription during either initiation...themselves. SunTag, SAM, and VPR have all shown significant improvements upon the initial dCas9-VP64 method...Performance Generally, VPR is found to have significantly higher activation levels than the initial dCas9...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ..., this method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with...it quickly saw wide interest, its dimeric nature can interfere with some applications. Several labs have...

  5. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and...Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors in Genome Editing Resources...

  6. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...different fluorescent proteins. Visualizing fluorescence can be challenging in plants because of the auto-fluorescence...prejudice about it. They just start and go ahead. I can see science speeding up. Addgene: Are there other... be equally high as for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning...

  7. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...fashion. Addgenies swapping out printer cartridges can place the spent cartridge in the large cardboard box ...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...and recycling in the event the object in question cannot be washed or reused/repurposed in some fashion....

  9. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...of transposons. Insertion of the mini-transposon can occur in two different orientations, but is strongly...encodes the mini-transposon. pEffector and pDonor can also be combined in single-plasmid INTEGRATE (pSPIN...was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical...guest blogger Leo Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical...

  10. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...Endotoxin Endotoxin contamination in vector preparations can alter the immunogenic properties of the final product... after negative staining. Empty vector particles can be identified after negative staining and appear ...
Showing: 801 - 810 of 892 results