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We narrowed to 904 results for: CAN

Showing: 801 - 810 of 904 results
  1. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ... are trickier to identify because their contents can be at various stages of degradation. Additionally...GFP-tagged version of LC3, the mammalian Atg8p homolog, can also be used to monitor autophagy, but under some...
  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...fluorescently labeled dCas9 to a given genomic locus, you can track the localization and movement of this locus...three hairpins generates white light. All 6 gRNAs can be expressed in a single vector, pCRISPRainbow-DONOR1...chromosome capture suffer from low resolution and can’t be used in vivo. Some researchers have used fluorescently...localization of 6 chromosome-specific loci in the U20S cancer cell line./ Each color represents a gRNA targeting... do so in vivo. Catalytically dead Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed...
  3. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...out, especially since videos on synthetic biology can be difficult to find. I hope that many people will...
  4. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...with a specificity far greater than the one that can be achieved using pharmacological or electrophysiological...on the other side, are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch ...opsin expression in specific neuronal cells? This can be achieved using Cre-dependent viruses, such as ...subsequently photo-stimulated through fiberoptic cannulas directly implanted in the injection site and connected...
  5. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...editing at frequencies comparable to SpCas9. SpRY can also edit NYN PAMs, but with less efficiency and ...will have a PAM located next to it. The SpRY Cas9 can also work with NYN PAMs; it just isn’t always ideal...compatibility. This alternative PAM requirement adds a significant amount of flexibility compared to a strict single-compatibility...increases the flexibility of gRNA placement significantly over SpCas9 and more so than Cas9s with an NG...
  6. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...each target has a unique species or isotype that can be probed with a distinctly conjugated secondary ...antibody. Consider too that some secondary antibodies can cross react with other species. For example, some...
  7. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...our “Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies... order well in advance whenever possible, as we cannot expedite the application process.  More information...
  8. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...answer any questions, and get feedback on how we can be more helpful to our community.  You'll also find...
Showing: 801 - 810 of 904 results