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TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....HDR). Cpf1 also expands the number of sites that can be targeted by CRISPR to AT-rich regions or AT-rich... so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and...activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche et al. found two that display robust... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...
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The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....to build relationships with their colleagues. It can be easy to get caught up in the minutia of the workday... that minutia gets much more manageable when you can enjoy the people you spend 40+ hours with each week...culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the question...is certainly not the truth (partially because I can’t whistle). No, we’re not all BFFs. Yes, sometimes... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m.... of the laser sheet and using multi-view imaging can provide enhanced resolution (8,9). Thus, LSFM is...adeno-associated virus)-based fluorescent labeling methods that can be used with 3DISCO to image non-transgenic animals...to photobleaching. Furthermore, point-by-point scanning along the entire specimen makes imaging too slow...photo-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition is performed... milliseconds to acquire one image, thereby significantly reducing the time it takes to image through ...Similar to light microscopy, there have been significant advances in tissue clearing techniques during... -
New Podcast Segment: Hot Plasmids
TypeBlog PostPublishedMay 17, 2017, 3:37 p.m....Plasmid articles with each of our newsletters. You can find all of these articles on the Hot Plasmids webpage... -
Our New Antibody Mascot is....
TypeBlog PostPublishedFeb. 24, 2022, 2:15 p.m....antibody joining us on our scientific adventures. You can submit your name suggestions here or by using the...best? But between these options, we simply can't decide! Abi, Lin, or Blottie? Which name best fits...we’re looking for a gender neutral name (and it can’t hurt if it’s also a great pun.) Submissions are ... -
#DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La
TypeBlog PostPublishedDec. 2, 2021, 2:15 p.m.... have a collection of snowman figurines that you can stage in a lab diorama. Or maybe you want to create... -
New Viral Vectors - Spring 2025
TypeBlog PostPublishedApril 29, 2025, 1:30 p.m....serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted viral vector reports... -
Podcast: A Malawian Professor's Path to Biotech Research
TypeBlog PostPublishedMay 24, 2016, 2:30 p.m....Listen to Additional Addgene Podcasts Learn How You Can Contribute to the Addgene Blog Learn about Addgene... -
2018 #DeckTheLab Contest Winners
TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....Instagram Eppi the red nosed Reindeer! 🦌🦌 as you can see lots of work always gets done this time of year... -
New Viral Vectors - March 2024
TypeBlog PostPublishedMarch 5, 2024, 2:15 p.m....serotype. We've also added two viral vectors that can constitutively express JEDI-2P, deposited by the ...