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...our quality control process for incoming plasmids can vary based on the contents of a plasmid, the first...translated amino acid sequence from within Snapgene, we can confirm the identity of the gene and check for any...any mutations. Once we identify the gene, we can easily annotate it by making a custom feature in Snapgene...multiple sequences at the same time. This means we can easily compare and analyze any differences between...plasmids are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions...require additional verification experiments that can be partially designed using the Snapgene desktop ...Snapgene’s “Import primers from a list” function, we can detect all of the primers we have designed that will...

...out as drugs can remain present until cleared by the body. Administering the drug once can result in several... high degree of temporal resolution as receptors can be turned on and off simply with the presence or ... is immediate, on the scale of milliseconds, and can precisely link a neuron's activation to behaviors...manipulation Both optogenetic and chemogenetic tools can have their expression targeted to specific tissues...broad tissue or system-wide targeting, as the ligand can reach target cells throughout the body. Controlling...better control over these factors as the light source can be easily and quickly manipulated or turned off. ...chemogenetics, while different concentrations of ligands can be injected, this is not as precise and the offset...

...from the event can be found below along with a description of the program beneath it. You can jump to the...replicates leading to suspect statistical significance (you can read more about this problem in Button et...preparation for my talk and at the minisymposium. You can find a variety of additional resources and information...mean that someone has replicated it precisely. You can find a great discussion of different types of replication... we’ll call a study reproducible if its findings can be predictably applied to future work. Importantly...solutions based on what I’ve learned. I hope you can apply some of these solutions to your own research...vectors. When working with a plasmid from Addgene, you can be confident that you’re using the actual tool that...

...customer problems. Technical support can be highly rewarding but can also be a good jumping off point for...obvious at rather late stages (i.e. postdoc level) and can include the pressure to publish in high-impact journals...there that require exactly what life science PhDs can offer: vast practical lab expertise, experience in...may have to answer technical enquiries such as: “Can I use this product under conditions different from...might be involved in such a role. In addition, it can be advantageous to speak multiple languages - that... For particularly tricky cases, team discussions can often lead to otherwise unobtainable solutions. To...essential for success. For possibly this same reason, it can be one of the more constant and safe corners in a...

...tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of camaraderie...I'm looking to see if there are any resources that can help with the transition.” The lab-to-desk-job culture...have as well. Making the transition from the bench can be jarring for the unprepared, and unfortunately,...academic research lab involves a few key features that can be difficult to replicate in other work settings:...experimental dry spells. Losing that sense of discovery can be hard since it’s frequently why many people get...most enjoyable aspect of their chosen career. You can confidently call yourself a Scientist. You work in...getting used to. Just remember: change is good. You can experience a real onboarding process. Unless you ...

...simple presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk...  When you buy a pooled library from Addgene, you can either order a DNA pooled library, which contains...lab, there are custom viral prep services that can prepare the libraries for you.  Starting from plasmids...verification, and lentiviral production steps before you can start transducing your virus. Let’s start there! ...functional titer is determined, larger lentiviral preps can be used for screening-scale transduction of cells...mutated by the viral pooled library. These cells can then be used for genetic screening, followed by sequencing... don’t. The size and composition of your library can drastically impact what conditions it needs to properly...

... homologous regions can be encoded in the primer sequences. Multiple plasmids can be included in a single...epitope tag can be moved to the N-terminus of a protein-coding sequence, or a mutation can be introduced...However, while not widely known, plasmid assembly can be achieved in vivo using a bacterial recombination...reagents or purification kits, it is low cost and can be immediately adopted by any laboratory. All procedures... Fragment generation - Any linear DNA fragments can be assembled in vivo as long as they have homologous...Using specific primer design (Figure 2), IVA cloning can be used to perform any plasmid modification, from...whole vector. The new codon and homologous region can be included at the 5’ end of either primer. Subcloning...

...network is to be always thinking “How can I help?” and not “What can you do for me?” One of the easiest ... connections can connect you to people at that organization. This type of connection can help you gather...interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include news, inclusion...messaging, not everyone likes to. A direct email can be the best way to get in touch if you have business...t really know, follow instead of connecting. You can see what they post and occasionally comment on their... people, this option is in the “More…” menu. You can also follow companies and organizations to see their...fair game for connecting to others.  Pro tip: You can change the option for sending LinkedIn messages to...

...packaging vectors for larger genes - they can hold up to 8 kb, while AAV can only carry around 4.5 kb. Different...Behaviors you can study using viral vectors By modulating neuronal gene expression and activity, you can use viral...development of viral vectors, behavioral neuroscientists can now precisely control where and when certain genes...required, because seemingly tiny external factors can have a quite noticeable influence on the outcome ... several days preceding the experiment. Handling can however negatively interfere with certain tests and...and affect behavior. A fact to consider: Rodents can hear ultrasonic frequencies (> 20 kHz, produced from... left behind from a preceding animal (ex: urine) can be distracting. If it is not cleaned, animals tested...

...measure how many of the viral particles produced can actually infect cells. These assays typically involve...several commercially available p24 ELISA kits that can quantify p24 levels in a matter of hours. A major...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct...backbones; once validated these universal primers can be used to titer any lentiviral prep that shares ...p24 assay, titering via measurement of viral RNA can overestimate the amount of infectious virus in a ...defective particles. Studies have found that values can be 10-1000 fold higher than for functional assays...from amplification of the original plasmid which can over-inflate results. A packing vector deficient ...