Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 790 results for: Cre

Showing: 81 - 90 of 790 results

  1. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ... pXPR_070 Violet-excited GFP (vex) Lineage-Cre; Rosa26-Cas9 S-CHIME Knocks out pairs of genes...of genes in immune cells typically involves the creation of germline knockout mice, which is time-consuming...cells can be implanted in irradiated recipients to create mice with gene knockouts in their immune systems...2019). A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nature Communications...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...specific system for controlling gene expression such as Cre-lox, may be sufficient to overcome the transport ...ability to produce neuron specific transfection using Cre-lox, this new method could allow for labeling of ...AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 ...looks like at 3 days vs 7 days vs 14 days etc. To decrease the time until expression, it is possible to use...

  3. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff/Fon-sRGECO...of biosensors could be used in high throughput screening for agrochemical compounds that have the potential...

  4. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...limiting amount of AAV are injected into the brain of Cre-expressing mice to trigger recombination and expression...site upstream of the stop cassette results in decreased efficiency of recombination and preferentially...

  6. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and...called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21... 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators... in-vivo imaging of voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral... Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here! ...

  7. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...stochastic multicolor labeling, Brainbow used the Cre-loxP system to express one of the three fluorescent...Addgene #104110 - #104112) (Figure 2). We’ve also created constructs with containing chemical tags such as...

  9. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...added flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC). To make...have used PCR-based screening to detect mutants, but this method limits screening to a few hundred worms... less than eight hours worth of hands-on time. Creating a modular, selection-based gene tagging approach...rare events difficult. Dickinson et al. sought to create a new and efficient selection-based CRISPR/Cas9...construct will serve as the repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The...knock-ins. These candidate animals can then be further screened based on their Roller phenotype. If SEC is inserted...separates the targeted gene from its promoter, creating a loss-of-function allele as well as a promoter-FP...

  10. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ... Resources on the Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Interview...artificial intelligence (including a team that created a novel form of AI that can learn new tasks and...
Showing: 81 - 90 of 790 results