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We narrowed to 630 results for: Son

Showing: 81 - 90 of 630 results

  1. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...difficult to find solutions to. There are many reasons why client situations might be especially challenging...skills is a must to be successful in this field. Personally, I have greatly benefited from and appreciated...when their teams are facing a crunch, and using lessons from previous cases to better staff future teams...conflicting work styles, team situations, and personalities. Teams striving to achieve a lot of great output...coach and counsel employees on the different personality and work styles (e.g., Myers-Briggs). Staff are...teams, to leverage the preferences of different personality types, and to devise strategies to be prepared...

  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...BRET= Bioluminescence Resonance Energy Transfer CRET= Chemiluminescence Resonance Energy Transfer    Interesting...now well-known FRET, aka Fluorescence (Förster) Resonance Energy Transfer, technique which allows the detection...have any cross-talk but you also don’t have any resonance transfer. You have to find a balance between FRET...downloads/modules/FRET_teaching_module.pdf Day RN, Davidson MW. Fluorescent proteins for FRET microscopy: ....201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET) microscopy imaging of live...

  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...the question of how much data was necessary and reasonable. PLOS has since clarified their data sharing ... in having exclusive access to data and enjoy reasonable time to make sense of it, with their decision...The real cost of making data accessible A more reasonable concern with the data policy was raised by researchers...how results were obtained and this alone is a reasonable motivation for increasing both data accessibility...published data. The importance of data sharing The reason this topic is important to my lab is three-fold... welcomed. However, expectations must also be reasonable, sensitive to the data type and made as facile...

  4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...with Gibson assembly. While this is a comprehensive mutagenesis method, a drawback to using Gibson assembly...plasmids ready for deep mutational scanning.     3 reasons to be interested in deep mutational scanning Hundreds...rate of mutagenesis, and low throughput.       Gibson Assembly: An easy-to-use technique that joins homologous...especially single basepair deletions. Therefore, Gibson assembly is better suited for creating small, focused... PubMed PMID: 27723752.  4. Fowler, Douglas M., Jason J. Stephany, and Stanley Fields. “Measuring the ...

  5. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...2015b) under a CC-BY 4.0 license. Long et al., Nelson et al., and Tabebordbar et al. each chose to try.... used SpCas9 in conjunction with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter ...not subject to nonsense-mediated decay. Although Nelson et al. observed only 2% genome editing in one experiment...persist long-term. In accordance with this result, Nelson et al. find that dystrophin restoration is maintained... E, Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing partially restores ...Dec 31. pii: aad5725. PubMed PMID: 26721683. 3. Nelson CE, Hakim CH, Ousterout DG, Thakore PI, Moreb EA...

  6. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ...path. Because there is no demand for a favor, the person you ask will be more likely to say yes and more... Informational Interviews are best conducted in person. Perhaps you can offer to buy coffee or lunch at... your interviewee? It is always best to meet in person to develop relationships, but if it is not possible.... Keep in mind that this is a good time to ask personal questions about salary, opportunities for advancement...advancement, challenges and disappointments. These more personal questions can elicit the most valuable insights...

  7. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...secrets - during a collaboration, members of Maureen Hanson’s and Rima Menassa's labs made the accidental discovery...created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the unexpectedly...Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to red photoconversion of GFP for protein... PubMed. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible...from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...DNA. Finally, in 1953, Franklin along with James Watson and Francis Crick resolved the DNA double helix...engineering itself is attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island...movies. Rampage (2018), starring Dwayne “The Rock” Johnson, calls out CRISPR as the genetic engineering experiment...Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR (2019) Search-and-replace...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher Correction: Programmable...:3870–3879 . https://doi.org/10.1093/nar/gkf495 Watson JD, Crick FHC (2003) Reprint: Molecular Structure...

  9. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... should I try research? Personally, I have always been a pretty curious person. I like to ask questions...anyone else in the world knows it—to be the first person to make a discovery. I can’t tell anyone else what...place to begin. Here’s my advice: Reflect on your personal interests While it’s perfectly fine to be unsure... also makes for an experience more tailored to personal interests. Talk to people, ask questions, and ...First-Generation College Students. Social Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/...

  10. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...and Hoess, 1984; Van Duyne, 2001; Christenson Wick and Krook-Magnuson, 2018). Optogenetic FLEx vectors ...PubMed PMID: 11340053. Wick, Zoé Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and...Nature 351.6326 (1991): 489. Hirsch, Matthew L., Sonya J. Wolf, and R. J. Samulski. "Delivering transgenic...PMCID: PMC5111696. Towne, Chris, and Kimberly R. Thompson. "Overview on research and clinical applications...
Showing: 81 - 90 of 630 results