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We narrowed to 644 results for: Uty

Showing: 81 - 90 of 644 results
  1. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...co-founders of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering ... as food but support GMOs that result in a new utility, was there a business opportunity out there for...changed color throughout the day. It would be a beautiful GMO, one that people would actually want to have...out of our careers: to build something new and beautiful, to engage with the public in a meaningful way... much knowledge and experience that goes into executing a successful business that the learning curve ... in the United States Air Force. She left active duty to pursue a degree in a synthetic biology laboratory...
  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...Creating xCas9 through directed evolution Hu et al. used phage-assisted evolution to generate and select for...of phage-assisted evolution, generating xCas9 2.0-2.6. This second round of evolution included continuous...lab, have gone one step further, using directed evolution to create xCas9, an enzyme recognizing a broad...broadest-targeting variants. For the final round of evolution, they used a no-G PAM library to enrich for Cas9...
  3. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...Collectively, the pool of CRISPR software embodies solutions that facilitate most experimental applications... engineering. When compared to that collective utility, it’s easy to fault the lack of features in a single... sgRNA design tools into database and de novo solutions. Database tools allow us to view and get a sense... workbenches such as MacVector or Benchling. Utility functions: These functions help speed up the sgRNA...single-method design”. Trends: The most common utility functions are batch design or multiplex features...repertoire. Organism agnostic tools are the better solution but are often packaged as offline tools requiring...use. The R based package CRISPRseek offers great utility and coupled with Bioconductor should be the first...
  4. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... DNA and proteins in solution. The pH is then lowered using a renaturing solution, which causes the proteins...genomic DNA to precipitate. Plasmid DNA is free in solution. Proteins and genomic DNA are removed by centrifugation...placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it ...: nucleic acids are broken out of cells with a solution of salts, detergent, and ball bearings. Then a...nucleic acids, so placing the dipstick into a wash solution removes contaminants while nucleic acids stick...
  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...refers to a saturated solution of formaldehyde (saturated being 37%). Formalin solutions often contain methanol... a fixative. However, when PFA is dissolved in solution and heated, it depolymerizes, producing formaldehyde...formaldehyde. Often people will call the resulting solution PFA, but it’s really just formaldehyde. Formalin...polymerization of formaldehyde.  Note: Formaldehyde in solution will naturally polymerize and lose its ability...the best results if you use fresh formaldehyde solutions.  Along with preserving your sample, cross-linking...
  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...create. Wildlife biology and ecology can make for beautifully compelling photos and videos, while molecular...platforms can splinter audience. Primarily digital distribution (though radio is possible.) Written works...works Low Flexible. Low barrier to entry. Ample distribution channels both digitally and physically. Easy...overwhelming your audience. Digital or print distribution.  Static. Can be difficult to convey an entire...highlighting, or omitting details. Digital or print distribution.  Static. Often requires both artistic and scientific...overlooked as a means of scicomm. Digital or print distribution.  Typically limited to a single page of information...
  7. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...get online and take a look at the company or institution’s career page, where you’re likely to find current...conference or job fair, or it may be someone at your institution’s career center. Alternatively, it might even ...other graduate students and post-docs from your institution have gone, and try sending them a professional... professional development, not count on your institution, program, or adviser to do it for you. Expose...for you to attend!), exploring a job fair, and utilizing your local career development center, if one exists... exists. Get online! Review company and institution career pages. Make yourself visible on LinkedIn and...
  8. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera Labs.              ...automate plasmid sequence assembly, and possible solutions for each in the form of one-off scripts or programs...how effortless it was for us to switch between executing our pipeline on a local computer or in a cloud... each week!), we already planned to use cloud computing resources. Now, we could iterate our development...criteria that we needed. Prior to using Nextflow, we routinely wrote Python scripts, queried our database with...known as channels. Since a Nextflow process can utilize any scripting language that can be executed on ...
  9. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...convergent evolution because electric organs arose in different branches of the evolutionary tree at least...freshman in college I studied development and evolution of histone genes in Strongylocentrotus purpuratus... and sperm, mix them in a tank, and observe the beauty of embryo development in real time. I was already...Breviolum minutum symbionts are important models for solutions to the coral reef crisis. I like how they are ...Chytrid fungi are useful for the study of basic evolution of fungi and eukaryotes and includes the fungus...environments so can be used study the genetic basis of evolution in immunology, neurobiology, and more. Prochlorococcus...
  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...2005-2023. We identified and offered a number of solutions that increased accessibility, such as using couriers...Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 Japan Leehyo Bioscience...country, and we can see a growth in material distribution after we partnered with them (Figure 2). In ...duration of their journey. Figure 2: Annual distribution of items to countries with a distributor. Arrows... some form of problem-solving. When universal solutions aren’t enough to ensure accessibility, partnering...formatting all 101s on a particular topic into beautifully designed and easy-to-reference eBooks. Figure...
Showing: 81 - 90 of 644 results