We narrowed to 697 results for: Tec

...excellent Technical knowledge and creativity Become a “thought leader,” develop a technical “niche,” develop... something (a journal, a science lunch club, a biotech club) Be the head of a group (like a grad student... develop a useful new assay, master a new technique and be able to talk and own these accomplishments....committee) Follow industry publications like FierceBiotech to “learn the business” Attend events with local...

...come up. An MTA can resolve those questions! MTAs protect you and your institution, and almost all universities... and adopted by the Association of University Technology Managers (AUTM). This MTA provides many benefits...rights – and the rights of your institution – are protected while we handle all the paperwork. Record ...Addgene is internationally recognized as being protective of both the providing institution and the receiving...receiving institution. Therefore, many technology transfer officers are familiar with the UBMTA and are...

...engineering company, Genentech, was founded.  This commercialization of gene editing technology led to the development... Diego. The revolution in genetic engineering techniques is a speculation of yesteryear which has been...double-strand breaks (DSBs) in the genome. These technologies rely on the cellular DNA repair machinery, which...genetic engineering and open advertisement of the technology on various non-scientific platforms, CRISPR has...perspective of the current. Although genome editing technologies have brought a renaissance in the field of health...

...use. In order for this technology to survive the diverse demands of the biotech field, let's look at how...survival tactics of prokaryotes to deliver the technological biological blockbuster known as the CRISPR (...various innovative biological and translative technologies. Addgene has empowered researchers to harness... idea or application that involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo,...

...eukaryotes! The technology has been used to optimize protein secretion and expression in the biotech workhorse...once you are up to speed on the basics of the technology. In this blog, we will highlight some common ...applications and kits. Keep in mind that this technology is useful for a variety of purposes beyond what...Heterologous Proteins in Yeast Mitochondria.” BMC Biotechnology, vol. 17, no. 1, Springer Science and Business... Enables Rapid Assessment of Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023...

...in government, evidence to inform policies, a protected place to work, funding for science, and numerous...cannot prosper in a world where our medicines, technologies, defense, and governing policies are developed...Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also studies energy markets in ...water. Many marchers held signs demanding the protection of our Great Lakes and our planet. And as we ...the truth about our world. Jonathan Aguilar (@AggleDetector on Twitter) is a graduate student at Johns Hopkins...being taught to communicate in a very specific, technical manner, so much so that we often find it difficult...

...photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation Irreversible...super resolution imaging, where the power of the technique relies heavily on the same fluorophore being imaged... and electron microscopy (CLEM) is an imaging technique that combines electron microscopy (EM) with fluorescence...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve...fluorescent protein induced by blue light." Nature biotechnology 24.4 (2006): 461-465. PubMed PMID:16550175. ... dual color fluorescence nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040. PubMed PMID:18724362...

...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...towards enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be...Because we’re conveniently located next to a major Biotech hub, building up our lab was easy. Most of our ...engineer yeast to glow, produce synthetic scents, detect compounds in the environment, and openly publish...Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter...

...tools by making their own fluorescent protein technologies widely available to the research community. ... a key advantage over other inducible enzyme technologies. She hopes that other researchers will be able... HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in the first case and... two biosensors that could quickly and reliably detect HMOs. Fatima’s first design is a whole cell biosensor...Fatima specifically proposes using this sensor to detect HMOs found on cellular surfaces. After incubating...

...Silva, Universidad de Ingeniería y Tecnología (UTEC), Peru Julio Ernesto Valdivia Silva is a master of interesting...identifying new drug targets. An early and accurate detection of PZA-resistance is necessary to improve tuberculosis...engineering: glowing bacteria and a fish robot to detect water pollution Dr. Julio Ernesto Valdivia Silva...synthetic biology resources and microfabrication technologies to generate a self renewable sensor, a bacterium...

...experimented with adding different proteins to the CGBE architecture. They conducted a CRISPRi screen to identify...forms a chemical crosslink with DNA abasic sites, protecting them from cellular processes that would cause...target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/...optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt...targeted DNA transversions in human cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587...enable C-to-A and C-to-G base changes. Nature Biotechnology, 39(1), 35–40. https://doi.org/10.1038/s41587...

...it’s true that adding histones and chromatin architecture to every diagram portraying some aspect of eukaryotic...when I recently attended the 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium...posters I saw at the 2018 Keystone Chromosome Architecture and Chromosome Organization. These aren’t the...Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role for a nuclear noncoding RNA: NEAT1 RNA ...epigenome. If you have been considering some new techniques, the posts below may steer you in the right direction...

...quite impressed by how neuroscientists are using techniques from across different disciplines to better understand...many opportunities to learn about and develop technologies that will have impacts on many different facets...ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around the poster... addition to the researcher using sequencing technologies to study microbiome interactions with Alzheimer's... or maybe your favorite FRET-based biosensing technique can be applied to an important neuronal signalling...

... cellular targets Optimized genetic screening technique using Cas12a for multiplexed gene perturbation...s41467-019-10846-1.   Optimized genetic screening technique using Cas12a for multiplexed gene perturbation... To validate this optimized genetic screening technique, researchers identified a synthetic lethal interaction...future genetic screens.   DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn...tags, are optimized for SDS-PAGE gels and can be detected on Western blots via engineered immunoglobulin...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...direct approach isn’t giving you enough signal to detect your protein of interest, it may be time to look...occasion, usually after other signal amplification techniques have been ruled out.  Ease of access Signal amplification...course, this is much easier said than done! Each technique, and sometimes each individual assay, will need...

...and success. Customer and technical support Our customer and technical service brands continue to ... We score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our...also lauded by another set of stakeholders - the tech transfer community. In a recent law review article...our image in the world. Addgene is now as much a tech company as it is a reagent company. I used to have...

...K855A) and eSpCas9(1.1), followed by a technique for detecting genome-wide double strand breaks (BLESS...it’s obvious that CRISPR is a ground-breaking technology that’s already had a huge impact on research ...effects on various biological disciplines, the base technology itself is constantly improving. Cas9 variants... "High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects." Nature (2016...

...recombination. What is Cre-lox? The Cre-lox system is a technology that can be used to induce site-specific recombination... lethality. To facilitate the use of Cre-lox technology, transgenic mice have been constructed that express...system is another site-directed recombination technology very conceptually similar to Cre-lox, with flippase...analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to Cre-lox, and...have any tips or tricks, or clever cre-dependent techniques?  Let us know in the comments! References 1....

...President's Council of Advisors on Science and Technology [PCAST] (2012). Engage to Excel: Producing One...Additional College Graduates with Degrees in Science, Technology, Engineering and Mathematics, Washington, DC:...DC: US Government Office of Science and Technology. 3. Duina, A. A., Miller, M.E., & Keeney, J.B. Budding...BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques High School Students Explore Careers at Addgene...Molecular Biology Reference Pages Learn Simple Lab Techniques in Our Protocol Videos ...

...on to find out more about this amazing imaging technique and find further tips for using FRET in your experiments...proteins is not optimal, FRET may not occur or be detected even if the two proteins form a complex. To help...a potential source of error in suboptimal or undetectable FRET, well-characterized FRET reference standards...environment which alters the FRET efficiency. Detecting certain cellular changes with an intermolecular...the concentration or activity of small molecules, detect cellular processes and signaling cascades, quantify...