We narrowed to 662 results for: Clu

...proteins that are found in and on everything, including your skin. Here are some general tips for working...degrade RNA. Keep a clean work area, which may include spraying your bench down with a product to get ...

...recognized for its biotechnological applications including conversion of CO2 into water and electrical energy... in cyanobacteria. This kit contains 96 parts including terminators and promoters, CRISPRi parts, selectable...

...proteins potently inhibit type II CRISPR systems including the popular S. pyogenes Cas9 (Rauch et al., 2017... the resulting optogenetic plasmid tools which includes plasmids encoding a fusion between cPYP and tgRFP...

...ahead of most companies in creating a welcoming, inclusive workplace for everyone. We had Unbiasing Training...movement. We are carefully attentive to genuine inclusion, but not worried because we are ahead of the curve...

...combinatorial expression of other genetic elements including transcription factors (Cheng et al., 2014) and... with effective gRNAs are publicly available, including those found at Addgene! Figure 1: Strategy...

...your expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save...plasmid from its predecessor. Important features may include cloning junctions (where most sequence assembly...

...sites around the USA undertaking BEST programs, including local schools in Boston: the Boston University...market. Her many notable contributions in this area include publishing the book ‘How Economics Shapes Science...

...for a successful grant application. It typically includes overview information, key dates, funding description...deliver outsider opinions, and reveal items to include for the next application. Although some comments...

...plasmids! This category includes genes that help bacteria invade hosts, including attaching to host cells...

...destroy. One treatment currently in clinical trials includes Cas3 (the shredder member of the Cas family) as... Other treatments currently in clinical trials include, but are not limited to, ones targeting muscular...

...widespread use of FRET with fluorescent molecules, including fluorescent proteins, has led to the alternative...measuring the change in FRET efficiency, from which conclusions regarding the proximity of the two proteins can...

...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test... negative control to a sample with the primary included to confirm that the signal is from the primary...

...local gene expression, confounding any potential conclusions about the role of methylation (Hrit et al. 2018... many effects independent of dCas9 targeting, including modifying other sites in the genome and directly...

...Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. These are ...are wells on the same ELISA plate that include known concentrations of your protein of interest. The optical...

...of the Retroviridae or Retrovirus family, which includes the gamma-retrovirus and lentivirus genera, have... field of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, ...

...Arg-mutant proteins in living baker’s yeast cells, including Arg-mutant yeast homologs of human proteins that...frequently found in genetic testing for syndromes that include developmental delay, result in loss of function...

...order to see an effect. Hemizygous mice can be included in breeding strategies identical to those used...with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan, or infertility...

... infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and ...can also carry different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification...

...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...published in a paper, but positive outcomes can include poster presentations, talks about your work, or...

...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and...mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation...

...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct ...infectious virus in a prep as the measurement will include defective particles. Studies have found that values...

...biologists working together on a variety of projects including the OpenWorm project. The main goal of OpenWorm... track them. We have a number of microscopes, including an automated microscope but we need to develop...

...conducting literature reviews and having weekly journal clubs outside of work. Our scientific training was put...we had an idea, knew how to build it, but had no clue how to run a business. SynBio Axlr8r thought color-changing...

...AAV6 is reported to have broad tissue tropism, including skeletal and cardiac muscle, airway epithelial...be expressed Cre-dependently to allow tracing exclusively from genetically determined cells. This version...

...website and also for offline use via GitHub (which includes prediction in batch mode). To predict the best...passion for computational biology. His expertise includes applying machine learning techniques to make sense...

...compensation between each other. We can therefore conclude that this population is double-positive for both...that there is no issue with compensation here. Conclusion Compensating between your fluorescent markers...

...standard quality control assays for AAVs. These steps include measuring viral titer and assessing purity. But...also conducting more rigorous quality control, including next generation sequencing (NGS) of the viral ...

... maintaining stable lines of genetic mutants, including in situations where maintaining heterozygosity..., they have fly collections for in vivo work, including CRISPR tools. The Vienna Drosophila Resource...

...used for colocalization analysis. Some examples include the ImageJ plugins JACoP and Coloc 2, BioImageXD...to the properties of FPs themselves. These can include the potential to oligomerize, protein charge, and...

...marker like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we...recombination using a repair template. These templates can include loxP sites to create conditional floxed alleles...

...introduce yourself and what you do. You might want to include your university and your advisor’s name for some... uploading your work to a preprint server (and include this information on the poster) so your visitors...

...possible, it uses standard tools and databases, including primer3, biopython, bwa, samtools, bowtie, UCSC...CrispyCrunch, be sure to check out the How To Guide. Conclusion CrispyCrunch is now publicly-available for high-throughput...

...gRNAGFP. Gonçalves says that advantages of AdVs include their episomal nature and very efficient introduction...using the Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: ...

...in the screenshot below). Currently, the page includes interactive maps for the Ras, mTor, and p53 pathways...from the research community, will soon expand to include several other important pathways. We invite feedback...

...are needed to optimize yield. You might want to include an extra wash step or use a different strain. For.... Look out for carbohydrates Certain strains, including HB101 and derivatives, release large amounts of...

...improvements? You could publish a paper that happens to include your improvements, or maybe even a methods paper...variety of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid...

...mammalian production system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese ...specifically. Possible applications for validation include western blot, immunohistochemistry, and immunocytochemistry...

...One of the cool things about amp is that it's exclusively toxic to dividing cells. The other antibiotics...confers resistance to several aminoglycosides, including G418 which is commonly used for eukaryotic antibiotic...

...– it makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and...which have fragments of the AdV genome in them, including the E1 region). The space in the viral particle...