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Showing: 401 - 450 of 790 results

  1. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...complement our written protocol materials, we have also created video protocols.  These feature step-by-step instructions...below: How-to videos Our How-To videos feature screen-capture footage to help you navigate Addgene’s ...the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden SciComm with the Experts at Science...

  2. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...understand the features of the plasmids, how they were created, and how to use them in experiments. Best practices...magical Addgene badge appear? First you’ll need to create an account on PubMed. Once you have an account,...populate your “PubMed filter list” to the left of your screen. That’s it! Next time you log-in to your PubMed...

  3. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...science has been hot this year and how do you creatively turn it into an outfit?), and of course there...bragging rights for the next 12 months. Your team’s creativity, cleverness, and crafting skills have surpassed...in 2012. The Office Team was back up to their creative hijinks last year. They showed us their dedication...

  4. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene would be successful...plasmid distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The...Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie...

  5. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...publication. Accessibility is surely also behind the increase in citations for older publications. (As an interesting...most cited. And, as scientific discourse has increasingly moved online, surely times have changed. Altmetrics.org...what impact looks like and what it’s made of. Increasingly, journal publications alone aren’t enough. Scientists...

  6. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    Published
    Feb. 25, 2021, 2:15 p.m.
    ...take this initiative to the next level with the creation of the The Professional Development Hub (pd|hub...drive change. This effort is already resulting in concrete actions and outcomes. For example, pd|hub assembled...collections of evidence-based practices. These concrete programs and practices to promote PhD professional...

  7. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ... to stress altogether. Their muscles showed an increase in inflammatory cytokines as a result of those...turns out that activation of the PGC-1a1 pathway increases skeletal muscle expression of kynurenine aminotransferases... proteins and metabolites that are dynamically secreted by muscle during exercise to meet the brain’s ...

  8. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ... in order to do the thing you love, you have to create opportunities. If you find yourself asking, "Why...position exist yet?", you are in the early stages of creating an opportunity. That's how Addgene was founded...That's how my position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management...

  9. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A flexible and...different effectors. I’d encourage people to get creative with them for their own uses.” Konermann said ...found here.) So tell us, readers, how will you get creative with LITE? Reference: Silvana Konermann et al...

  10. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...longer allowing myself to just simply exist without creating a storm. Given all the options out there, I was...the company. One of the things I wanted to do was create content that was unique but also brand friendly... a new design program, Canva, and I was able to create cute and on-brand templates for these posts. ...

  11. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... developed SECURE cytosine base editors, which decrease the rate of off-target genome edits compared to...adenine and cytosine editor). Target-ACE exhibits increased editing efficiency of a target site compared to...advantages like reduced off-target activity and decreased immune response to Cas9. Recently, Baisong Lu’...

  12. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...million in annual R&D expenditures to support increased public access to the results of federally-funded...recommend Eric Lander's intro to biology course "The Secret of Life." 10)     And, last but not least (certainly...know your favorite ways to share science. Image credit: Judy Breck, via flickr  ...

  13. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ... we created one long lunch table and we eat together Harry Potter-style.The shared lunches create a culture...or says something about my cooking.   This is a creative (and delicious) team building activity that is...

  14. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates around 30...likely to be most useful for labs that already have created a transgenic GFP-containing organism,” says Hanson...

  15. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...holds yearly planning meetings with each trainee to create a supportive, growth-oriented lab culture. The ...perfectionists,” and we don’t often give ourselves credit for what we have accomplished. Starting with a ...meetings make the lab run more smoothly. With a concrete plan in hand, trainees are motivated to complete...

  16. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...that your protein might not fold well, you should screen a few different vectors to see which gives you ...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...

  17. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... today. Although I wish I could take all of the credit, the success of the company is attributed to the... of an interactive game that Addgene employees created and designed for the Hall of Human Life at the ...Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination to keep materials...

  18. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...Use software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and... computer or smartphone. Evernote allows you to create notes directly from webpage clippings, photographs...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the...

  19. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ... interacting partners and perform whole animal screens for small molecules that block the neurodegenerative...their expression in specific cells. With a more concrete understanding of the molecular biology behind ...and pathways to target in future small molecule screens. He plans to work with a partner at IRCCS Besta...

  20. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox, Ma et al. turned to SpCas9...was fused to a different fluorescent protein to create three colors. The specificity of these orthologs...nucleome a specific priority, as researchers work to increase our understanding of chromatin organization. This...

  21. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...The last trick GeoCas9 has up its sleeve is its increased longevity in human serum. Harrington et al suspected...shown that a cocaine esterase engineered to have increased thermostability also had a longer lifetime in ...Harrington, Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma." bioRxiv (2017): 138867...

  22. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...your project's potential. Widening your search creates fertile ground for serendipitous encounters that...research and PhD studies can enrich the depth and creativity of your work, while offering mental health benefits...but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries. So...

  23. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained by M. ...University of Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA. ...repository for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines...

  24. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... the connection between LEGOs and science more concrete to help further unlock the potential contained...process One major challenge when we started was creating a LEGO stage that resembles a real laboratory.... your experiments to think outside the box, be creative, and put your inner child to work. If you have...

  25. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...provide advice. The society organizes numerous recreational and educational events throughout the year, ...iGEM alumni-industry networking to promote the creation of start ups and biotech spin offs. The Synthetic...Inspiring future scientists At Addgene, we’re incredibly glad to see these biological societies engaging...

  27. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ... these meetings in relatively isolated resorts creates a sense of camaraderie with fellow attendees from...depositor, discussed some of the many challenges of creating biological circuits, and I can only say this science...opportunity to share their cutting edge science in increasingly important field.  Interested in Addgene's ...

  28. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ..., how can cells with that mutation be selected/screened? A4: Before starting your experiment, consider...sequencing, for example) on each colony in order to screen for positive ones.  -- OR -- If your HR template...would like to thank Le Cong for all his hard work creating amazing resources for the scientific community...

  30. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. The fixed...detected over a 10-week period. Their expression decreased over time but they were not silenced.    Find ...retroviral overexpression cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell...

  31. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...University of Alberta and University of Oregon created mTFP1 by transforming E. coli with a gene library...tetramer-breaking mutations. The library was then screened through successive rounds of mutagenesis for the...filter. This combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a ...

  32. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...R6K, and 15A. Not all origins of replication are created equal. Some will produce many plasmid copies and...because they will compete for the same machinery, creating an unstable and unpredictable environment. As ...plasmids if they contain large inserts or genes that create a toxic product. The E. coli strain Most E....

  33. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...single-strand DNA incorporation (ssDI). "Easi-CRISPR for creating knock-in and conditional knockout mouse models...Protoc. 13(1) (2018):195-215. PubMed PMID: 29266098 Creating knock-in mouse models with CRISPR has been challenging...efficiency. Easi-CRISPR uses a long ssDNA template to increase efficiency of insertion and gene replacement. ...

  34. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...collection of antibodies available at launch were created by James Trimmer, PhD, of the University of California...commitment to supporting your research, Addgene is creating an array of educational materials to accompany...more useful to everyone over time. Through our incredible community, including your support, we have enabled...

  35. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...to select against CRISPR positive plants, but screening these can be challenging and requires specialized...Willoughby   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under...as positive selection of transformed plants to decrease the routine usage of antibiotics which may contribute...

  36. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...primary antibody and endogenous IgGs in the tissue. Created with biorender.com. Strategies for avoiding...Some labs have found that you can significantly decrease background staining by mixing your primary and...you add your primary and secondary antibodies. Created with biorender.com. Proper tissue processing...

  37. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma antibody...antibodies against the new Fc region. In addition to increasing multiplexing capabilities this process is frequently...Although isotype conversion is routinely used to increase the utility of recombinant antibody tools, the...

  38. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ... - not just for fiction! Image credit: Kenneth Rowe, under the creative commons license.    The introduction...is to find one or two roadblocks that sparked creativity, changed the outcome of the project, or illustrates...

  39. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...we found that adding a TEV linker dramatically increased accessibility of the AviTag and HisTag, which ...fusion protein in bacteria. Our initial attempt to create a plasmid based on the biotin ligase BirA backbone... inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available ...

  40. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...cassette allowing a researcher to perform blue-white screening after transformation - self joining of the vector...break down the steps needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need...unpaired 3’ ends in your PCR product. If you need to decrease error rate, please use one of these methods to...

  41. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am lucky... your potential as an entrepreneur, not your credentials. If you have an idea, don’t wait.  Go for it....

  42. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane into the culture medium...AAVs (Schiller et al., 2018). Exosomal vesicles secreted from producer cells also contain cellular macromolecules...open new ways of improving gene delivery. They increase the efficiency of transduction, broaden the use...

  43. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...to keep the audience engaged through a computer screen. For the Reproducibility for Everyone Workshop,...author of the poster,” he says.Viewing a poster on a screen is challenging, requiring you to zoom in to different...canceled may be disappointing, but there are many creative ways to adapt and take advantage of the new conference...

  44. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ... the stem cell community – a community largely created due to Yamanaka’s seminal work. It didn't take... during his keynote address, appeared almost incredulous that the field has exploded so quickly, citing...explained that it took 50 people nearly 2 years to create the first stem cell line that could be used in ...

  45. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ..., described below, are typically employed when creating stable cell lines and are not used for general...Stable transfection A stable transfection is used to create a population of cells that have fully and successfully...culture media until 24-48 h post transfection when creating stable cell lines. Looking for Mammalian Expression...

  46. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...communication skills with training programs, which are increasingly available. But think of this as a jumpstart ...possible outcome of a ComSciCon workshop is the creation of a new science communication initiative that...science communication opportunities in your area, create them.  Seek out collaborations with organizations...

  47. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...use small, incremental changes to understand what works and what doesn't. By being creative, thoughtful...each step as you complete it. Yes, this can be incredibly tedious, but it also works, so do it anyway.  ...

  48. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...emitted yellow-green light and exhibited 3-5 fold increased brightness over previous BRET probes, with sufficient...injected intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous... reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent ...

  49. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...production. Finally, we are blessed to have an incredibly supportive Board of Directors at Addgene. Our...leadership of Addgene’s co-founder, Melina Fan, we created a new team to push this project forward. The viral...you to the Addgenies who went the extra mile to create this new service. We knew the scientists were waiting...

  50. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ... vector and insert are (separately) digested to create 10–12 base “chewed back” overhangs, they can be...bacteria, which will efficiently repair the plasmid. Created with BioRender.com.   SLIC also opened up a...transformed into bacteria, which repair the plasmid. Created with BioRender.com. How does SLIC compare to...
Showing: 401 - 450 of 790 results