We narrowed to 803 results for: Cre

...Experimental output is measured and quantified. Created with BioRender.com.   Optogenetic tools Types...passing ions against the concentration gradient. Created with BioRender.com.   Opto-G-protein coupled...the binding pocket and activates the receptor. Created with BioRender.com.   Controlling secondary...LOV domain allowing the pathway to be activated. Created with BioRender.com.   Important considerations...

...be cloned into E. coli and subsequent proteins screened for cell-binding abilities (6). This can be accomplished... repair sheared ends with T4 DNA polymerase (to create blunt ends) and ligate into a bluntly digested ...construction for functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 ...Protein-Protein Interactions Use CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology...

...individuals wherever the cell cultures are kept will increase the risk of contamination. Mycoplasmas can spread...even though the viability of cryopreserved cells decreases with every subculture. Equipment Fumigate the... not be stored in a single incubator since it increases the risk of cross-contamination. CO2 incubators...Elimination is a very time-consuming process and increases the risk of secondary contamination to other healthy...

...IWGAV five proposed antibody validation pillars. Created with BioRender.com.     Orthogonal Strategies...knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...opposite of the Genetic strategy and looks for increased antibody signal in samples that have had the target... target protein expression experimentally increased. The simplest version would be to use cells that do...

...The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This book is published 30...character revolts against class divide that is created by genetics.    Zinc finger nucleases Zinc finger... it can read many base pairs simultaneously to increase specificity. This modular approach made ZFNs highly...space travels. The movie sees the USS Enterprise crew fight against a genetically engineered superhuman...

...public. Find out more at www.projbridge.com. Walnut Creek, CA, USA 37.9101°N 122.0652°W – Steph Hays When...microbial interactions, and science education. Photo cred Alina Chan. Seattle, Washington, USA 47.6062°N 122.3321...others come out who feel the same.  I was also incredibly impressed with how well organized the march was...was, as well as the friendly atmosphere that was created by the many thousands of science professionals ...

...Parallelized Confocal Microscopy (Spinning Disk) increases the speed at which a confocal image can be acquired...utilizes a configuration of two or more objectives to create a thin sheet of excitation light that propagates...molecules on and off. This can provide a 2-10 fold increase in resolution, down to 10s of nanometers. Thick...samples due to its fast temporal resolution and decreased phototoxicity. Lightsheet designs that allow for...

...more photons are absorbed and then emitted, which creates a relatively stronger — or brighter — signal.   ...brightness levels on your smartphone or computer screen), and you should be able to properly label many...included—the live/dead stain. This stain is in fact not created through the actual APC-Cy7 fluorophore but displays...absorbed and emitted by matter, and how this process creates visible colors. Next, you learned about how fluorophores...

...year’s impressive entries, but our community of creative scientists delivered some spectacular photos once...supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament ever! & they...winners. We hope everyone had a blast decorating and creating memories with their labmates! Are you inspired...

...the world to help increase global access to materials in our repository. This incredible gift will allow...a los materiales de nuestro repositorio. Este increíble regalo permitirá que más personas accedan a recursos...

...complement our written protocol materials, we have also created video protocols.  These feature step-by-step instructions...below: How-to videos Our How-To videos feature screen-capture footage to help you navigate Addgene’s ...the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden SciComm with the Experts at Science...

...understand the features of the plasmids, how they were created, and how to use them in experiments. Best practices...magical Addgene badge appear? First you’ll need to create an account on PubMed. Once you have an account,...populate your “PubMed filter list” to the left of your screen. That’s it! Next time you log-in to your PubMed...

...science has been hot this year and how do you creatively turn it into an outfit?), and of course there...bragging rights for the next 12 months. Your team’s creativity, cleverness, and crafting skills have surpassed...in 2012. The Office Team was back up to their creative hijinks last year. They showed us their dedication...

...a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene would be successful...plasmid distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The...Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology Group in Palo Alto, CA.  Benjie...

...publication. Accessibility is surely also behind the increase in citations for older publications. (As an interesting...most cited. And, as scientific discourse has increasingly moved online, surely times have changed. Altmetrics.org...what impact looks like and what it’s made of. Increasingly, journal publications alone aren’t enough. Scientists...

...take this initiative to the next level with the creation of the The Professional Development Hub (pd|hub...drive change. This effort is already resulting in concrete actions and outcomes. For example, pd|hub assembled...collections of evidence-based practices. These concrete programs and practices to promote PhD professional...

... to stress altogether. Their muscles showed an increase in inflammatory cytokines as a result of those...turns out that activation of the PGC-1a1 pathway increases skeletal muscle expression of kynurenine aminotransferases... proteins and metabolites that are dynamically secreted by muscle during exercise to meet the brain’s ...

... in order to do the thing you love, you have to create opportunities. If you find yourself asking, "Why...position exist yet?", you are in the early stages of creating an opportunity. That's how Addgene was founded...That's how my position and my team at Addgene was created.”- Eric Perkins, Director of Science Product Management...

...optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A flexible and...different effectors. I’d encourage people to get creative with them for their own uses.” Konermann said ...found here.) So tell us, readers, how will you get creative with LITE? Reference: Silvana Konermann et al...

...When attempting to use the CRISPR/Cas9 system to create specific mutations or insertions by Homologous ..., how can cells with that mutation be selected/screened? A13: Before starting your experiment, consider...sequencing, for example) on each colony in order to screen for positive ones.  -- OR -- If your HR template...would like to thank Le Cong for all his hard work creating amazing resources for the scientific community...

... developed SECURE cytosine base editors, which decrease the rate of off-target genome edits compared to...adenine and cytosine editor). Target-ACE exhibits increased editing efficiency of a target site compared to...advantages like reduced off-target activity and decreased immune response to Cas9. Recently, Baisong Lu’...

...longer allowing myself to just simply exist without creating a storm. Given all the options out there, I was...the company. One of the things I wanted to do was create content that was unique but also brand friendly... a new design program, Canva, and I was able to create cute and on-brand templates for these posts. ...

...million in annual R&D expenditures to support increased public access to the results of federally-funded...recommend Eric Lander's intro to biology course "The Secret of Life." 10)     And, last but not least (certainly...know your favorite ways to share science. Image credit: Judy Breck, via flickr  ...

... we created one long lunch table and we eat together Harry Potter-style.The shared lunches create a culture...or says something about my cooking.   This is a creative (and delicious) team building activity that is...

...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...necessary to observe a signal. Red signal intensity increases with GFP concentration and saturates around 30...likely to be most useful for labs that already have created a transgenic GFP-containing organism,” says Hanson...

...holds yearly planning meetings with each trainee to create a supportive, growth-oriented lab culture. The ...perfectionists,” and we don’t often give ourselves credit for what we have accomplished. Starting with a ...meetings make the lab run more smoothly. With a concrete plan in hand, trainees are motivated to complete...

...that your protein might not fold well, you should screen a few different vectors to see which gives you ...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...

... today. Although I wish I could take all of the credit, the success of the company is attributed to the... of an interactive game that Addgene employees created and designed for the Hall of Human Life at the ...Addgene means to them. Ken mentioned Addgene’s incredible growth and our determination to keep materials...

...Use software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and... computer or smartphone. Evernote allows you to create notes directly from webpage clippings, photographs...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the...

... interacting partners and perform whole animal screens for small molecules that block the neurodegenerative...their expression in specific cells. With a more concrete understanding of the molecular biology behind ...and pathways to target in future small molecule screens. He plans to work with a partner at IRCCS Besta...

...response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox, Ma et al. turned to SpCas9...was fused to a different fluorescent protein to create three colors. The specificity of these orthologs...nucleome a specific priority, as researchers work to increase our understanding of chromatin organization. This...

...The last trick GeoCas9 has up its sleeve is its increased longevity in human serum. Harrington et al suspected...shown that a cocaine esterase engineered to have increased thermostability also had a longer lifetime in ...Harrington, Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma." bioRxiv (2017): 138867...

...plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained by M. ...University of Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA. ...repository for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines...

...your project's potential. Widening your search creates fertile ground for serendipitous encounters that...research and PhD studies can enrich the depth and creativity of your work, while offering mental health benefits...but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries. So...

... the connection between LEGOs and science more concrete to help further unlock the potential contained...process One major challenge when we started was creating a LEGO stage that resembles a real laboratory.... your experiments to think outside the box, be creative, and put your inner child to work. If you have...

...provide advice. The society organizes numerous recreational and educational events throughout the year, ...iGEM alumni-industry networking to promote the creation of start ups and biotech spin offs. The Synthetic...Inspiring future scientists At Addgene, we’re incredibly glad to see these biological societies engaging...

...Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon on the right?!? ...’t have to mean abandoning it or running away screaming, but instead can involve walking alongside it ...it and giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share ...

... these meetings in relatively isolated resorts creates a sense of camaraderie with fellow attendees from...depositor, discussed some of the many challenges of creating biological circuits, and I can only say this science...opportunity to share their cutting edge science in increasingly important field.  Interested in Addgene's ...

...the community, increasing the impact of the work, and as a collateral benefit, increasing the impact of...Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, and Nature, 1945–1990." Notes and...

...individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library. The fixed...detected over a 10-week period. Their expression decreased over time but they were not silenced.    Find ...retroviral overexpression cocktail yielded a three-fold increase of fully reprogrammed cells based on single-cell...

...University of Alberta and University of Oregon created mTFP1 by transforming E. coli with a gene library...tetramer-breaking mutations. The library was then screened through successive rounds of mutagenesis for the...filter. This combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a ...

...single-strand DNA incorporation (ssDI). "Easi-CRISPR for creating knock-in and conditional knockout mouse models...Protoc. 13(1) (2018):195-215. PubMed PMID: 29266098 Creating knock-in mouse models with CRISPR has been challenging...efficiency. Easi-CRISPR uses a long ssDNA template to increase efficiency of insertion and gene replacement. ...

...R6K, and 15A. Not all origins of replication are created equal. Some will produce many plasmid copies and...because they will compete for the same machinery, creating an unstable and unpredictable environment. As ...plasmids if they contain large inserts or genes that create a toxic product. The E. coli strain Most E....

... - not just for fiction! Image credit: Kenneth Rowe, under the creative commons license.    The introduction...is to find one or two roadblocks that sparked creativity, changed the outcome of the project, or illustrates...

...collection of antibodies available at launch were created by James Trimmer, PhD, of the University of California...commitment to supporting your research, Addgene is creating an array of educational materials to accompany...more useful to everyone over time. Through our incredible community, including your support, we have enabled...

...to select against CRISPR positive plants, but screening these can be challenging and requires specialized...Willoughby.   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under...as positive selection of transformed plants to decrease the routine usage of antibiotics which may contribute...

...primary antibody and endogenous IgGs in the tissue. Created with biorender.com. Strategies for avoiding...Some labs have found that you can significantly decrease background staining by mixing your primary and...you add your primary and secondary antibodies. Created with biorender.com. Proper tissue processing...

...Recombinant antibodies are plasmid-based and are created synthetically or derived from hybridoma antibody...antibodies against the new Fc region. In addition to increasing multiplexing capabilities this process is frequently...Although isotype conversion is routinely used to increase the utility of recombinant antibody tools, the...

...we found that adding a TEV linker dramatically increased accessibility of the AviTag and HisTag, which ...fusion protein in bacteria. Our initial attempt to create a plasmid based on the biotin ligase BirA backbone... inserting BirA into a pMal plasmid (NEB) thus creating the pMal-T-Avi-His/BirA plasmid now available ...

...cassette allowing a researcher to perform blue-white screening after transformation - self joining of the vector...break down the steps needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need...unpaired 3’ ends in your PCR product. If you need to decrease error rate, please use one of these methods to...