We narrowed to 528 results for: Set

...re-administration but I do not think the field has settled on ‘best practices’ for model organisms.    Q11...expression is when injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...

...et al   Multiplexed Lentiviral Expression Cassettes lentiviral transduction One vector expresses... own promoter   Kabadi et al Csy4-Cleavable Cassettes transfection gRNAs expressed from a polycistronic...co-transfection of Cas9 Nissim et alTsai et al PTG Cassettes transfection gRNAs expressed from a polycistronic...

... an organism uses only a subset of codons, it only needs to produce a subset of charged tRNAs and therefore...increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of tRNAs that...

...in adult animal models results in rapid symptom onset, suggesting that MECP2 is also essential after development... gene was disrupted and silenced by a Lox-Stop cassette, resulting in the neurological deficits observed...Restoring the MECP2 gene by excision of the Stop cassette using tamoxifen-inducible Cre not only halted ...

... activating chemogentic receptor (hM3Dq) into a subset of neurons within the LC of mice. When those neurons...harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression...further and showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible...

...and deposit individual documents or more complex datasets into the desired repository. They also support...experiment documented in RSpace using the RSpace datasets deposited in Figshare or Dataverse has access ...electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged to be part of the vibrant...

...courtesy of IPI. IPI anti-integrin toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies... that bound an amino acid region displayed by a subset of the RGD-binding integrins containing the αV ...

...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP...eliminating two color possibilities. The remaining cassette can invert as long as Cre remains expressed; once...

...responses to viral infections have resulted in the onset of cancer. Similar patterns of cancer development...inflammatory bowel disease is associated with the onset of colorectal cancer. Strategic immune evasion mechanisms...cytotoxic effector T cells, and targeting specific DC subsets in vivo using specific antigens to activate them...

...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate...molecular biology tools out there! Here, we highlight a subset of these tools for plasmid mapping, DNA/protein...

... in the dataset; 2) users not part of a scientific Twitter list were excluded from the dataset. 3. 60%...

... (a specific mutation in a known gene = disease onset) may be best served by this type of targeted design...hits’ - genes that affect disease progression or onset. These hits can reveal individual genes or sometimes...

...recycle our discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don... for smaller and smaller gel apparatus until I settled on using glass slides. With just the right amount...

... only lead to a better understanding of disease onset, but also better simulate a disease in a model system... custom TF programs to acutely initiate disease onset, providing both a tool to test prophylactics on ...

...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...

...found to have a point mutation in the puromycin cassette that makes it less efficient in some cell lines... It contains a point mutation in the puromycin cassette that makes it less efficient in some cell lines...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The modularity...

...career, I’d have realized it was a great fit for my skillset and interests. As such, I want to be able to share...re building software will help you be a valuable asset to the scientists as well as the developers. Lastly...

...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... Weiss’s group used these reporters to define a subset of active miRNA’s unique to HEK293FT and HeLa cell...

...RNAs (gRNAs) are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and ...and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit...

...then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty...characterized by increased iron serum levels and early-onset cataracts, is characterized by point mutations in...

...biochemistry department at the University of Massachusetts Medical School. We are working with postdocs...to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary...

...COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from Ando et al. 2023, under CC-BY...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...

...carries CRE recombinase and a puromycin resistance cassette. No time to produce virus? Get the ready-to-use...Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher titer...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...excision or insertion, inversion, translocation or cassette exchange. By utilizing recombinases to manipulate...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... hybrid gRNA library deletes human alternative cassette exons and disrupts genes with alternative exons...

... website so scientists can view public circRNA datasets and download the custom python scripts needed ...phage vectors with CRISPR/cas9 sensitizing gene cassettes. Thse vectors are engineered to replace the drug...

...while lentivirus requires a BSL-2 setup. This requires a less involved setup when working with mice and slightly...

... used to compare between different experimental setups. A colocalization analysis example is given in ...detection of one fluorescent signal in a channel setup for a different fluorescent signal) and photobleaching...

...different names depending on your flow cytometer’s setup. For instance, BV510 and AmCyan have almost identical...practice, you may be able to do your compensation setup on your experimental samples without needing to ...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines are...

...). Misek and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic...by bias within the design of the experiment or dataset itself. It is important for scientists to not only...

...incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ...doi.org/10.1016/0378-1119(88)90117-5  Lale, R., Brautaset, T., & Valla, S. (2011). Broad-host-range plasmid...

...doi.org/10.1006/mthe.2000.0116 Gutierrez-Guerrero A, Cosset F-L, Verhoeyen E (2020) Lentiviral Vector Pseudotypes...Salmon P, Gay W, Nègre D, Le Grand R, Trono D, Cosset F-L (2002) Lentiviral vectors pseudotyped with ...

...GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across 8,792 organisms...biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...

..., and are therefore able to infect only a small subset of the cells used in a given experiment. For this...Porteus. "CRISPR-mediated integration of large gene cassettes using AAV donor vectors." Cell reports 20.3 (2017...

...complement each other to restore the full-expression cassette. AAV’s natural ability to reassemble fragmented...2017) CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756...

..., we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest...kb or less. Luckily, most of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc...

... Its Fruit Is Sweet Time If you require a quick onset of expression, AAV may not be the best tool to use...delivery indicates ~7-21 days are required for the onset of gene expression. However, it has yet to be determined...

...gene of interest with an antibiotic resistance cassette.   Substrate DNA Design and Generation Whether...expression of the recombineering system.   This setup can be also be used to modify chromosomal genes....

... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP...

...photoactivatable CRISPR gene activation system, but settled on a different optimal fusion protein combination... the protein’s ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen in the...

...for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect the CRISPR...pluripotent stem cells differing exclusively at two early onset Parkinson Point mutations. Cell, 146(2), 318–331...

...travel to Addgene's headquarters in Watertown, Massachusetts, for the occasion! Addgenies enjoying the ...

...yourself how the AAV performed in that experimental setup. For example, this is an experiment using the chemogenetic...

...finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing temperatures...

...Alex Chavez, a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in ...

...Having seen so much in the business world from the mindset of a lawyer can bring a new perspective to an organization...

... digest, PCR, or transformation?  Great news. A subset of our collection has been prepared as part of ...

...synthetic pathway and assembled them into a synthetic cassette induced by one of several ligands. Plants showed...