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The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Oct. 22, 2015, 2:30 p.m.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Nov. 2, 2021, 1:15 p.m.

Everything you need to know about antibodies in this blog post! Get the facts about the types of antibodies and how they can be used in the lab.

Jan. 19, 2021, 2:15 p.m.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Oct. 27, 2020, 1:15 p.m.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon

Review of the most common types of affinity tags and there optimal usages.

Sept. 19, 2023, 1 p.m.

This post covers loading controls and normalization of semi-quantitative western blots. We cover housekeeping proteins and total protein loading controls.

April 8, 2025, 1:15 p.m.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Nov. 3, 2022, 12:15 p.m.

Pseudotyping is a method use to introduce a viral envelope protein from another virus to restrict or broaden host cell. Get the what, why, how of pseudotyping in this article.

April 20, 2021, 2:30 p.m.

There are many method to identify protein-protein interactions. Learn about different method of proximity labeling based on biotin-streptavidin interactions.

Dec. 2, 2019, 2 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.

With this NIH's BRAIN Initiative Grant, Addgene will provide ready-to-use recombinant antibodies and will build a crowdsourced antibody Data Hub.

May 25, 2021, 2:29 p.m.

Find tips for hosting a great virtual lab meeting with ideas for online collaboration and space for brainstorming and discussion.

Feb. 9, 2021, 2:15 p.m.

There are many ways to study Dopamine including in vivo microdialysis and genetically encoded biosensors. Read on to learn about two recently developed biosensors: dLight and GRABDA.

July 30, 2019, 12:59 p.m.

The Addgene viral team has found a way to increase AAV yields through media supplementation of sucrose, an endocytosis inhibitor.

Dec. 13, 2018, 1:14 p.m.

Tips on how to choose compatible fluorescent proteins for multi-color imaging.

Oct. 9, 2014, 3 p.m.

Western blots have many technical elements. We cover each element, and the different options available to choose from, in this post.

Aug. 22, 2024, 1:15 p.m.

Laura Drepanos from the Broad Institute shares the strategies her team used to optimize the CRISPR knockout libraries Jacquere and Julianna.

Sept. 8, 2026, 1:14 p.m.

Addgene has updated our useful CRISPR guide to include PRIME editing, new enzymes, and more!

Oct. 10, 2024, 1:15 p.m.