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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 704 results for: ANG

Showing: 161 - 180 of 704 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A., Wu,...generating variants with improved affinity and dynamic range of both nLightG and nLightR, and may soon have a...Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & Li,...

  2. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...stars Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist...CBE) use a cytidine deaminase to introduce a C→T change at precise locations in the genome. This was soon...Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus ...Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F (2013) Genome engineering using the CRISPR-Cas9...EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Meyer BJ (2011...

  3. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...chemistry. The society currently has 352 members, ranging from undergrad all the way up to professors. Oxford...Oxford currently has around 700 members and hosts a range of scientific meetings for students to attend. It...society provides talks and career support for a range of different scientific disciplines, as well as ... opportunities for society members as well as arranging collaborations and conferences for their students...Synthetic Biology Society also aims to develop a range of talks for both students and the general public...

  4. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...the success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG...without requiring a double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that...-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added to the...identification of essential MYC binding sites in a wide range of human cells.  A new pooled library from Junjie...

  5. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ... Research is Advocating for Change Within the Sciences Learn How Changing Labor Laws Can Effect Postdoc...editors) can move slowly, news about possible broad changes to policies seems to break everyday. Many researchers...active and involved if we wish to make positive change. Below I present perspectives on the March for ...cause autism to politicians saying that climate change is a hoax. This has culminated in the current President...before the House Science Committee about climate change. All the scientists – including global warming ...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...have attempted to study cancer from every possible angle to leave no stone unturned, but cancer has been ...age, infections, or other strenuous biological changes. In contrast to the acute immune responses that...other effector immune cells. TGFβ is also known to change CD4+ Th cells into regulatory cells to suppress... promotes the production of VEGF to increase angiogenesis around the tumor. This in turn regulates the...results in frequent physiological and morphological changes. Tumors differ in their genotypic and phenotypic... understanding of the genotypic and phenotypic changes in the various types of cancer will fuel the development...

  7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... of fluorescence: For applications that involve ranges of pH, and significant lengths of time, the stability...key to observing interactions and conformational changes in proteins. Both short distances of molecules ...offered at Promega. Due to the versatility of interchangeable labeling using the HaloTag® system, the new...s41592-021-01338-6 Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, ... S7–S7. https://doi.org/10.1038/ncb1939 Liu, X., Yang, L., Long, Q., Weaver, D., & Hajnóczky, G. (2017...Bitounis, D., Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers...

  8. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ... the New Research That's Rewriting the Story by Angela Saini "This book is a smart and carefully presented...benefit and sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator...Associate Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical...s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate degree...Vonnegut’s Slaughterhouse-Five, and the responses ranged from “one of my favorite books” to “I barely got...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...three additional amino acid changes were introduced to nCas9. These changes promote nCas9’s dissociation...molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify ...types of nucleotides. Both the Bassik Lab and the Chang Lab have created methods that introduce diversification...Cytidine Deaminase (AID) enzyme. AID, however, only changes cytidines or guanines to the other three bases,...

  10. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...our understanding of what accessibility is would change over the next twenty years. But learn we did! ... of Addgene’s content team, we — Jo, Brook, and Angela — most often think about accessibility in terms... and educational content conflicted with plain language guidelines.  Which is why, at the Society for ...when I’m done? What are we trying to fundamentally change about their knowledge?    Here, this image is being.... Other times, it is more helpful to use plain language and focus on a broad takeaway.  In this image,...

  11. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... can’t fix an experiment by simply running it unchanged over and over again, and the same goes for your...again (I’ve been there!), think of ways you could change your variables - your approach, your workflow, ...setup - to prevent the mistake, just like you would change variables to fix a failed experiment.  For example...work, and the possibilities for small, helpful changes are endless.    Fig. 2: Blugene moves ... experimental approach, use small, incremental changes to understand what works and what doesn't. By being...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...monoclonal antibodies. Genetic drift refers to changes in the nucleic acid sequence of the antibody-encoding...over time as the hybridoma cells divide. These changes can alter the antibody that is actually produced...produced by the hybridoma, meaning that there will be changes between lots over time. Specifically, the antigen-binding...antigen-binding sites of the antibody - the paratopes - may change and impact the specificity and avidity of the antibodies... a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within ...

  13. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...neuroendocrine, adipose, immune, inflammatory, angiogenic vascular, endothelial, and stromal cells - a ...lack of caveolin 1 in breast cancer also induces changes of stromal cells in the microenvironment. This ...transferred to other cells, inducing epigenetic changes in targeted cells. In this case, exosomes may transport...formation by inhibiting cell growth, invasion, and angiogenesis (Allinen et al., 2004). In fact, Sanford H. ...influence tumor microenvironments: immunology and angiogenesis (Witz et al., 2009). Then, scientists began ...

  14. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...go ahead with raises. What will these further changes mean? The abrupt reversal in salary raises at institutions...Continuing efforts to gather information and expedite change We are continuing to update our FLSA and Postdocs..., under “How institutional plans have/have not changed since the injunction”. Postdocs around the country...enterprise and promote their voices in driving changes in science. Follow him on Twitter @BiophysicalFrog...the Addgene Blog Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read ...

  15. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...Dolan, E., Wang, Hou, Zhonggang, Xiao, Yibei, et. al. Introducing a Spectrum of Long-Range Genomic Deletions...an expanding number of others. Best for: a wide range of DNA edits.   Cas3 – The shredder What sets Cas3... sticky ends. Cas12a cleavage results in 5 bp overhangs and is particularly convenient for targeting experiments...

  16. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...fluorescent protein tools. This year, we’ve made some changes. In addition to supporting in-person conference...health and disease,” says Aggarwal. This biosensor changes fluorescence based on the release of glutamate ...be transferred to the roGFP2 cysteine thiols and change its fluorescence properties. The biosensor can ...allow us to better understand how neuron activity changes following opioid withdrawal, providing insight ... Jennifer Tsang...

  17. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...viral components; according the manufacturer these changes result in yields up to 25x higher than other available...such as Cas9. See figure 1 for an example of how changing transfection ratios can affect transfection efficiency...assessing lentiviral titer, don't compare apples to oranges After choosing a packaging system and producing...line. To overcome this issue, we recommend using a range of doses of lentivirus when transducing a target... Oct 10;12(15):1893-905. PubMed PMID: 11589831. Jiang W. et. al.. An optimized method for high-titer lentivirus...

  18. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...precise insertion of a DNA sequence ranging from a single nucleotide change to large insertions like the addition...: The key components of Nanoblades. Image from Mangeot et al., 2019.   MLV GAG-POL polyprotein Nanoblades...efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar to other approaches... accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary...

  19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To see even more tools, head...and resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis E, ..., Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard ...bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna CM (2020) Open Sharing During COVID-19... Jennifer Tsang...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying the...working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition of the viral...viral vector with bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain... following transfection. In other words, when exchanging the medium after transfecting cells, replace ...
Showing: 161 - 180 of 704 results