...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor...PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing constructs, transcript...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...

...GFP. The lab tested the base editors in vivo and achieved therapeutically relevant editing efficiencies ...heart, and skeletal muscle. The dosing required to achieve these efficiencies was also between 1013 - 1014...Ace absorbance increases and results in higher quenching of mScarlet emission. Figure 2: Ace-mScarlet...

... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science...position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “There were jobs...applied and I got it.” Science writing at Fred Hutchinson Cancer Research Center Work as a Science Writer...

...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing...’s important to remember that the study hasn’t achieved any end goal not available with current techniques...

..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1-10) for use with..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama ECFP C155 of ECFP pairs with N173 derived..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama sfCherry2 sfCherry2(1-10) and sfCherry2...

...back into the infants. Almost all patients have achieved persistent immunological reconstitution with a...mutations in the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable...Improvement and decline in vision with gene therapy in childhood blindness." New England Journal of Medicine 372.20...

...intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting time and money on optimizing...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...

...Intermolecular FRET can be experimentally difficult to achieve, because the ratio of acceptor to donor fusions... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that...PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule...

...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...

...Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic algae, during heat...cost and resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment...doi.org/10.1186/s13036-018-0100-0 Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N.,...

... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...

... that this step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds...c-Myc protein and did not recognize the mouse or chicken homologs. By comparing the homologous sequences...Myc1-9E10 in the late 1990s (Fuchs, et al. 1997; Schiweck, et al. 1997) with the goal of making the antibody...

...writing a grant, thinking about the next project, reaching out to other teams, expanding your network…. I...problem, I was able to resolve this issue - with coaching - before it became a major problem. But I also...always the case or you may not feel comfortable reaching out to your manager. This is when having a mentor...

... instances of focused attention become magical teaching moments. The models function as thinking tools...that the resulting physical model is an effective teaching tool. You can read about Jacob’s initial reaction...BioMolecular Modeling web site to check out our hands-on teaching tools that are designed to make the molecular ...

...partially because they contain some of the necessary machinery to make these viruses. The cells are also easy...they require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus... https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H., Lim, Z. F. S., & Ng, S. K. (2021). HEK293...

...concentration. Add supplements to the media You can achieve higher cell density using a nutrient-rich broth...plasmids may need to be grown for 20 hours or more to achieve maximum plasmid yield. The optimal growth time ...insufficient gas exchange will prevent your cultures from reaching the desired density. The volume of your flask ...

... functional groups: nucleases, modulators, and chimeric effectors. Nucleases generate a classic double-stranded...break, while modulators regulate transcription and chimeric efforts make a precise edit or insertion via a...distributed CRISPR plasmids. But modulators and chimeric effectors, made available in 2013 and 2016, respectively...

... is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

...practice because FRET depends on a number of hard-to-achieve factors. For example, FRET requires that fusion...in large enough quantities (and the correct stoichiometric ratios) to generate useable data. What’s more...7. https://doi.org/10.7554/elife.38853 Hu C-D, Chinenov Y, Kerppola TK (2002) Visualization of Interactions...