We narrowed to 578 results for: CHI;

...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...  Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

...Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol...

...can be affected by both autofluorescence and photobleaching. Researchers have long been interested in using... cells. As Nano-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple...applications. For long-term imaging, especially where photobleaching and phototoxicity are a concern, these constructs...

...samples, the rest will be put into a status we call “Archived.” This status allows us to store plasmids and ...These new processes have been instrumental in achieving our mission to help science move faster in the...Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular Stomatitis Virus...

...brightness, Less signal distortion from photoswitching or photobleaching Improved signal-to-noise ratio (SNR...% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute...

... luciferase reporters aren’t susceptible to photobleaching — they’ll keep emitting light as long as their...Sensitivity Higher Lower Background Lower Higher Photobleaching/phototoxicity Not susceptible Susceptible ...advantages of luminescent assays (low background, no photobleaching) but provides flexibility by pairing the luciferase...

...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor...PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing constructs, transcript...

... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. Susan Keown’s science...position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “There were jobs...applied and I got it.” Science writing at Fred Hutchinson Cancer Research Center Work as a Science Writer...

...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing...’s important to remember that the study hasn’t achieved any end goal not available with current techniques...

...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...

...GFP. The lab tested the base editors in vivo and achieved therapeutically relevant editing efficiencies ...heart, and skeletal muscle. The dosing required to achieve these efficiencies was also between 1013 - 1014...Ace absorbance increases and results in higher quenching of mScarlet emission. Figure 2: Ace-mScarlet...

...back into the infants. Almost all patients have achieved persistent immunological reconstitution with a...mutations in the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable...Improvement and decline in vision with gene therapy in childhood blindness." New England Journal of Medicine 372.20...

...Intermolecular FRET can be experimentally difficult to achieve, because the ratio of acceptor to donor fusions... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that...PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule...

...fluorescent protein tags. Tamura et al. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1–10) for use with...fluorescent protein tags. Tamura et al. Commun Biol. 2021 Daichi Kamiyama ECFP C155 of ECFP pairs with N173 ...fluorescent protein tags. Tamura et al. Commun Biol. 2021 Daichi Kamiyama sfCherry2 sfCherry2(1–10) and sfCherry2...

...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...

...intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting time and money on optimizing...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...

... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...

...concentration. Add supplements to the media You can achieve higher cell density using a nutrient-rich broth...plasmids may need to be grown for 20 hours or more to achieve maximum plasmid yield. The optimal growth time ...insufficient gas exchange will prevent your cultures from reaching the desired density. The volume of your flask ...

...writing a grant, thinking about the next project, reaching out to other teams, expanding your network…. I...problem, I was able to resolve this issue - with coaching - before it became a major problem. But I also...always the case or you may not feel comfortable reaching out to your manager. This is when having a mentor...

... instances of focused attention become magical teaching moments. The models function as thinking tools...that the resulting physical model is an effective teaching tool. You can read about Jacob’s initial reaction...BioMolecular Modeling web site to check out our hands-on teaching tools that are designed to make the molecular ...