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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 659 results for: Clu

Showing: 161 - 180 of 659 results

  1. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ... planning to soon expand to other cell types, including more physiologically-relevant systems, to catch... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...navel-gaze over from the last couple of weeks, including whether or not scientists can recognize so-called...work on emerging issues in novel technologies, including self-driving vehicles, synthetic biology, biosecurity...factory with their cats, Harley and Lexi, and a clutter ofoutdoors-only spiders, all of whom are named ...

  3. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...RNA-seq clustering of cells transduced with the TF pooled library. Louvain community clusters of individual...Collection) and an accompanying expression atlas, including over 3,500 human TF isoforms, to help researchers...

  4. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...lifesaver when your research requires cell culture and include a counter, lab timer, dilution calculator, and ...quite helpful and provides a list of directions including which buffers to use and that indicates if a sequential...reagents you work with in experiments. Hence, I’m including this MSDS app. The Chemical Safety Data Sheets...

  5. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his first year....gradually incorporated regular group meetings, journal clubs, and focused sub-group meetings to discuss day-to-day... great resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide...

  6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...previously identified Cas13a-c orthologs, but they do include HEPN nuclease domains characteristic of the Cas13...these studies also identified other advantages, including Cas13d-specific regulatory proteins and high targeting... knockdown by CasRx for each of four targets, including two mRNAs and two nuclear long non-coding RNAs...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins....chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012...al. expanded the Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these...

  8. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag ...HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG...frankenbodies have a variety of applications, including live-cell imaging, single-molecule tracking, immunolabeling...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... fluorescence in the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium...world, there are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors...sensors designed for them. For example, this class includes tension monitors associated with specific proteins...

  10. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...target a particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that...the kit have the rest of the protein in place including FokI endonuclease domain as well as fluorescent...use as testified by the fact that the TALEN kit includes 834 plasmids for the pieces you need to assemble...

  11. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ... antibodies available (others you’ll encounter include monoclonal and recombinant antibodies). By the ...further antibody production, polyclonal antibodies include any antibodies produced during the immune response...staining you are seeing is real. It’s important to include a negative control using serum from the same species...

  12. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...an epitope folded into a space that would size-exclude an antibody. In imaging applications, smaller affinity...They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ...

  13. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...undergrad, I was assigned a paper to give as a journal club to my molecular biology class. A Cell paper. With...took just under a minute. I can't say the journal club was a rousing success–the response was a full sixty...with you. If you can give a seminar, lead a journal club, or present at lab meetings, you’ve developed a ...

  14. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...the quality of other services that we provide, including viral preps and pooled libraries. As anyone who...throughout the library preparation process and includes Picogreen quantification of various intermediate...particularly difficult to sequence and assemble, including GC rich regions. For example, the CAG promoter...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as CAR-T cells...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but ...recombinant antibodies as well? Our collection includes a mouse and a chimeric rabbit (original mouse ...

  16. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...overhangs, three or four base-pair sequences, and exclude or include specific overhang sequences, allowing it ...parameters. For even more specificity, you can exclude specific sequences or set a narrow search window...

  17. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...DREADDs DREADDs DREADDS, or designer receptors exclusively activated by designer drugs, are G protein-coupled...al., 2016).   DREADD ligands Several DREADDs, including hM3D(Gq), have been derived from muscarinic receptors... Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chemical Neuroscience...

  18. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ... of infection and rare, but severe, symptoms, including the development of fetal microcephaly and Guillain-Barré...seven institutions involved in the effort. This inclusive and open approach by all involved definitely contributed...transformed the assay’s capabilities. Specifically this includes primer directed isothermal RNA amplification, ...

  19. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...series of single- and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and ...detect action potentials and subthreshold events, including miniature excitatory postsynaptic potentials (...triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

  20. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... mice. While not an exhaustive list, these can include what’s being injected, the type of experiment, ...method. Many different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses.... the name suggests, in the muscle. Common uses include administering vaccines, pain meds, or other drugs...
Showing: 161 - 180 of 659 results