...protein, etc...).  There are many online guides – including an excellent series of posts by Joachim Goedhart...risk of inaccurate data submissions, so FPbase includes a versioning system that allows users to see when...improvement and elaboration.  Current projects include incorporation of biosensors, protein evolution ...

... Tom Ellis was offered a start-up package that included a postdoc and a PhD student in his first year....gradually incorporated regular group meetings, journal clubs, and focused sub-group meetings to discuss day-to-day... great resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide...

... fluorescence in the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium...world, there are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors...sensors designed for them. For example, this class includes tension monitors associated with specific proteins...

...nano-lanterns for use in various applications, including optogenetics, biosensors, and fusion proteins....chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012...al. expanded the Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these...

...previously identified Cas13a-c orthologs, but they do include HEPN nuclease domains characteristic of the Cas13...these studies also identified other advantages, including Cas13d-specific regulatory proteins and high targeting... knockdown by CasRx for each of four targets, including two mRNAs and two nuclear long non-coding RNAs...

...applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag ...HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG...frankenbodies have a variety of applications, including live-cell imaging, single-molecule tracking, immunolabeling...

...target a particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that...the kit have the rest of the protein in place including FokI endonuclease domain as well as fluorescent...use as testified by the fact that the TALEN kit includes 834 plasmids for the pieces you need to assemble...

...undergrad, I was assigned a paper to give as a journal club to my molecular biology class. A Cell paper. With...took just under a minute. I can't say the journal club was a rousing success–the response was a full sixty...with you. If you can give a seminar, lead a journal club, or present at lab meetings, you’ve developed a ...

... antibodies available (others you’ll encounter include monoclonal and recombinant antibodies). By the ...further antibody production, polyclonal antibodies include any antibodies produced during the immune response...staining you are seeing is real. It’s important to include a negative control using serum from the same species...

...an epitope folded into a space that would size-exclude an antibody. In imaging applications, smaller affinity...They are often used for clinical applications, including imaging.  Diabodies are ~60 kDa proteins formed...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal ...

...overhangs, three or four base-pair sequences, and exclude or include specific overhang sequences, allowing it ...parameters. For even more specificity, you can exclude specific sequences or set a narrow search window...

...DREADDs DREADDs DREADDS, or designer receptors exclusively activated by designer drugs, are G protein-coupled...al., 2016).   DREADD ligands Several DREADDs, including hM3D(Gq), have been derived from muscarinic receptors... Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chemical Neuroscience...

...nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as CAR-T cells...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but ...recombinant antibodies as well? Our collection includes a mouse and a chimeric rabbit (original mouse ...

...the quality of other services that we provide, including viral preps and pooled libraries. As anyone who...throughout the library preparation process and includes Picogreen quantification of various intermediate...particularly difficult to sequence and assemble, including GC rich regions. For example, the CAG promoter...

... of infection and rare, but severe, symptoms, including the development of fetal microcephaly and Guillain-Barré...seven institutions involved in the effort. This inclusive and open approach by all involved definitely contributed...transformed the assay’s capabilities. Specifically this includes primer directed isothermal RNA amplification, ...

...series of single- and double-tagged constructs (including "CLAP", combining an N-terminal CLIPf tag and ...detect action potentials and subthreshold events, including miniature excitatory postsynaptic potentials (...triggered by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...

... mice. While not an exhaustive list, these can include what’s being injected, the type of experiment, ...method. Many different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses.... the name suggests, in the muscle. Common uses include administering vaccines, pain meds, or other drugs...

... transferable into multiple bacterial species including several involved in human disease. However, the...every ~100 to 1,000 colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer...Mobile-CRISPRi expands CRISPRi to non-model bacteria including pathogenic strains and environmental isolates....

...the ATCC Declutter! Keep the hood clean Hoods should not be used as storage cabinets. Clutter not only...ll be brought under the hood with 70% ethanol, including your glove-covered hands! You should additionally...

...NgAgo optimization before we can make any major conclusions: That said, many people are having difficulty...Some great places to get your results out there include: Google Groups - I've specifically set up a forum...Medicine (CRM), Edinburgh. His research interests include optimizing genome-editing methods in brain cells...