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We narrowed to 364 results for: DUR

Showing: 161 - 180 of 364 results

  2. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...standard protocol, but the primary antibody is omitted during the incubation step. Samples are incubated with...controls address the spectral overlap that occurs during multiplexing. Compensation controls are single ...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    .... Leaving the membrane overnight in the freezer during one of these steps is also a convenient stopping...Szymanski lab (though she’s tagged along with the lab during its recent move to the University of Georgia). ...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, as it relies on...selection cassette can be incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR...

  5. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad school, you searched...own experience, great mentors often can be found during conversations with senior investigators. Discussions...

  7. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...interest in bacterial and archaeal immune systems. During this time, scientists discovered many related immune...(2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity. Cell, 161(5), 1164...

  8. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...Photostability, the ability to retain fluorescent intensity during exposure to light, is another factor to consider...facilitate clear differentiation between multiple dyes during analysis. Image resolution is impacted by several...

  9. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... visualizing structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All...amber i.e. UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid...

  10. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...There are a few different issues that can arise during the plasmid purification process. One is the possibility...from bottles and the overall weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch...

  12. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...to improve targeting specificity or reduce the duration of Cas9 activity.  The field of optogenetics is... fold induction at 31X. By using a slit pattern during blue light exposure (470nm), the researchers showed...

  13. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...pipetting! We’ll release a new tip every two weeks during the school year. Our first quick tip, “Your Bench...

  14. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    Published
    Jan. 7, 2020, 1:55 p.m.
    ...finding a good name for Blugene’s virus friend.  During the month of January, we’ll be taking name suggestions...

  15. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words of welcome...

  16. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ... we'll be giving away fun Addgene swag for the duration of the conference (be warned, the hand sanitizer...

  19. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one...
Showing: 161 - 180 of 364 results