...cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator to... the sequence of interest and an inactivated fluorescent RNA reporter. If the target sequence is present...programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA ...

...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth. Overgrown...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels...and discard it if the culture has become overly confluent or too old. Ensuring that you are working with...Martin, S., Merten, O.W., Galy, A., Fenard, D. ”Influence of mildly acidic pH conditions on the production...

... In practice, multiple sources of error (red) influence these relationships and must be addressed with...suggest that common housekeeping proteins are influenced by a variety of experimental conditions (Ghosh... that will be measured in your assay. This is influenced by sample concentration as well as by antibody... lane, your loading control won’t be strongly influenced by unexpected variations in any individual protein...

...Transparency also allows scientists to easily observe fluorescently labelled proteins and tissues to better assess... into the worm, such as animals that express fluorescent markers (Mello et al., 1991). Genes can also ... genetic modification, such as expression of fluorescent protein or disruption of a gene, can be easily...Read our quick guide to Drosophila Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...

...antibodies while FPbase curates information about fluorescent proteins so you can best apply these technologies...publication can lead to the publication of rare flukes showing positive results when really there are ...things that make it more likely that we’ll find a fluke when we’re simply trying to get our experiments ...Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific Figures...

... make. For example, large insertions, like a fluorescent protein knock-in, suffer from low efficiency ...with your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region ...most of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (...chromatin structure of the target locus may also influence efficiency. However, Quadros et al. saw success...

...understand and engineer cellular processes that are influenced by temporally ordered biochemical events. How...a unique combination of red, blue, and green fluorescent proteins in each state. The three methods of...mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages...opinion, the most convenient strategy is the fluorescent reporter based method since it lends itself to...

...Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous...mCyRFP1 - a new, monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot...: mScarlet - a new, bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet ...

...challenge. Chromoproteins are a subset of the fluorescent protein family that give off color in ambient...the Addgene blog Read our other blog posts on fluorescent proteins Check out educational resources on the... the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...

... interested in our fluorescent protein background. Download the orange fluorescent protein background....If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET ...

... many of the top 10 posts from this year were influenced by COVID-19 and our new cloning grade DNA service...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is...Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color! They'...

...stem cells Lindy Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform ... lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and ...that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific...

...(FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used...via applications such as western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is ...

...antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another solution...Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining. J Histochem...Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity Reagents Antibodies...

...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...secondary antibodies can be used with different fluorescent labels and both targets can be successfully visualized...

...categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually...would only then kill the desired plants! PCR or fluorescent markers are currently used in these situations...could be achieved much easier than by PCR or a fluorescent microscope. First, red CRISPR-positive transformants...

... often fall short with the generation of various flux imbalances. The conventional approach has typically... the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives of...strategy can be applied to effectively change metabolic flux. The dCas9 protein and orthogonal variants can...

... light responsive than fluorescent proteins required to get a good fluorescent signal. It may not be necessary...are often necessary to visualize expression of fluorescent proteins, for example, as many viral particles...

...bodies decorated with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed... body dynamics. Second, we showed that these fluorescent R bodies can rupture E. coli spheroplasts (cells...

...protein. OptoNBs were also tagged with an infrared fluorescent protein tag which let the team detect its co-...revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead, suggesting that OptoNBs...OptoNB-SOS activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These ...