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We narrowed to 383 results for: FLU

Showing: 161 - 180 of 383 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator to... the sequence of interest and an inactivated fluorescent RNA reporter. If the target sequence is present...programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA ...

  2. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...generally not true. Because of the variations in fluorescent signals, and how the machine reads/interprets...light that “scatters” past the cell, instead of fluorescent markers. There are various types of SSC and FSC...which is comprised of antibodies conjugated to fluorescent markers and/or cellular dyes and stains. These...anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers are used to identify monocytes (upper...

  3. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...broad host range plasmids. The properties that influence host range are not fully understood, but scientists...also control copy number and stability, which influence the plasmid’s ability to establish and maintain...Enterobacteriaceae, Gluconacetobacter xylinus, Haemophilus influenzae, Hypomycrobium X, Legionella pneumophila, Methylophilus...xylinus, Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum...

  4. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...subcultured at densities that are low enough to prevent confluency but high enough to promote cell growth. Overgrown...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels...and discard it if the culture has become overly confluent or too old. Ensuring that you are working with...Martin, S., Merten, O.W., Galy, A., Fenard, D. ”Influence of mildly acidic pH conditions on the production...

  5. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... In practice, multiple sources of error (red) influence these relationships and must be addressed with...suggest that common housekeeping proteins are influenced by a variety of experimental conditions (Ghosh... that will be measured in your assay. This is influenced by sample concentration as well as by antibody... lane, your loading control won’t be strongly influenced by unexpected variations in any individual protein...

  6. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...Transparency also allows scientists to easily observe fluorescently labelled proteins and tissues to better assess... into the worm, such as animals that express fluorescent markers (Mello et al., 1991). Genes can also ... genetic modification, such as expression of fluorescent protein or disruption of a gene, can be easily...Read our quick guide to Drosophila Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...

  7. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...antibodies while FPbase curates information about fluorescent proteins so you can best apply these technologies...publication can lead to the publication of rare flukes showing positive results when really there are ...things that make it more likely that we’ll find a fluke when we’re simply trying to get our experiments ...Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific Figures...

  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... make. For example, large insertions, like a fluorescent protein knock-in, suffer from low efficiency ...with your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region ...most of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (...chromatin structure of the target locus may also influence efficiency. However, Quadros et al. saw success...

  9. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...understand and engineer cellular processes that are influenced by temporally ordered biochemical events. How...a unique combination of red, blue, and green fluorescent proteins in each state. The three methods of...mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based) each come with their advantages...opinion, the most convenient strategy is the fluorescent reporter based method since it lends itself to...

  10. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    Published
    Sept. 8, 2017, 7:52 p.m.
    ...Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous...mCyRFP1 - a new, monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot...: mScarlet - a new, bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet ...

  11. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...challenge. Chromoproteins are a subset of the fluorescent protein family that give off color in ambient...the Addgene blog Read our other blog posts on fluorescent proteins Check out educational resources on the... the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...

  12. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ... interested in our fluorescent protein background. Download the orange fluorescent protein background....If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET ...

  13. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... many of the top 10 posts from this year were influenced by COVID-19 and our new cloning grade DNA service...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is...Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate color! They'...

  14. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...stem cells Lindy Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform ... lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and ...that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific...

  15. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...(FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used...via applications such as western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is ...

  16. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually...would only then kill the desired plants! PCR or fluorescent markers are currently used in these situations...could be achieved much easier than by PCR or a fluorescent microscope. First, red CRISPR-positive transformants...

  17. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...antibody that has your reporter (in this case a fluorophore) directly attached to it. (B) Another solution...Technique Adaptable to Biotin-Free Reagents, Immunofluorescence, and Multiple Antibody Staining. J Histochem...Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity Reagents Antibodies...

  18. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...secondary antibodies can be used with different fluorescent labels and both targets can be successfully visualized...

  19. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... often fall short with the generation of various flux imbalances. The conventional approach has typically... the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives of...strategy can be applied to effectively change metabolic flux. The dCas9 protein and orthogonal variants can...

  20. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... light responsive than fluorescent proteins required to get a good fluorescent signal. It may not be necessary...are often necessary to visualize expression of fluorescent proteins, for example, as many viral particles...
Showing: 161 - 180 of 383 results