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We narrowed to 285 results for: Fus

Showing: 161 - 180 of 285 results

  1. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...MoClo nomenclature and structure can be quite confusing for new users, and your cloning success depends...terminator, or other plasmid part? Do you want to fuse your protein to any tags or fluorescent markers?...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...versatile option for tagging proteins, as it can be fused to the N- or C-terminus (or even in the middle of...antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...triple nuclear localization signal or including fusions with Histone 2A or 2B can result in bright targeted...expression of single or multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor...

  4. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    .... A common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain...Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate to the nucleus where...

  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...video shows how to perform a Kirby-Bauer disk diffusion test and check bacteria for antibiotic resistance... treatment. This is called a Kirby-Bauer disk diffusion test or Kirby-Bauer susceptibility test (Figure...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest ...light to generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing...

  9. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or... a transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason...

  10. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...must wait for the opsin to express and passively diffuse down the length of the fiber before stimulation...are not actively transported and must passively diffuse so, depending on the length of your projection,...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...never used the Ex Taq. In our hands, Herculase II Fusion polymerase or Kapa Hifi Polymerase work very well... (meaning the mature processed form of crRNA is fused chimerically to a mature tracrRNA), the spacer used...

  15. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...

  18. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target...

  20. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... saturates around 30 uM. For low-abundance GFP fusions, a photoconversion signal may not be observed. ...
Showing: 161 - 180 of 285 results