We narrowed to 185 results for: GRN

...CRISPR plasmids for protein tagging Find validated gRNAs ...

...genomic locus in any cell type. By varying which sgRNAs are used with FIRE-Cas9, experiments can be designed...

...good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ...

...variable portion of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists...

... system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until...

... the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher...

...is mediated by recruitment of MS2-functionalized gRNAs.  Find the yeast CRISPRa plasmids here Reduce ...

...dedicated to expressing Cas9, while the other houses the gRNA and scaffold. If you don’t feel like doubling the...

...target DNA, recruited by either the ωRNA (TnpB) or a gRNA (Cas9). Drawings not to scale. Created with BioRender.com...

...of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral...

...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ...

...transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression...

...protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene...

...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine...

...Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ...

...Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene...

...Replication What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go...

...expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers...

...can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein, a fusion with GFP allows...

...linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave the 22kb vector. When followed...