We narrowed to 182 results for: GRN
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TypeBlog PostPublishedApril 23, 2018, 2 p.m....genomic locus in any cell type. By varying which sgRNAs are used with FIRE-Cas9, experiments can be designed...
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CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m.... the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a ~10-fold higher... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....variable portion of the plasmid in the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m.... system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....is mediated by recruitment of MS2-functionalized gRNAs. Find the yeast CRISPRa plasmids here Reduce ... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....of PE protein components (Figure 1), an altered epegRNA delivered with an enhanced pU6cm promoter, viral... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....dedicated to expressing Cas9, while the other houses the gRNA and scaffold. If you don’t feel like doubling the... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....transfect/inject cells with in vitro preassembled Cas9/gRNA complexes or with guide RNA- and Cas9-expression... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by George Church’s lab). Addgene... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools ... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....Replication What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled libraries at Addgene... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein, a fusion with GFP allows... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave the 22kb vector. When followed... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....Check out Our CRISPR Guide Pages Find Validated gRNAs to Target Your Genomic Locus of Interest Browse ... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology ...