We narrowed to 332 results for: NUC

...green cloud) functions both as a restriction endonuclease and as a ligase by cleaving and rejoining supercoiled... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....

...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ...RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional...

... and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm....to spectrophotometric methods that measure all nucleic acids). These methods are more sensitive than UV...

...and has previously been used for multiplex Cas9-nuclease editing. Typically, a single array encoding the...editing, such as base editing, prime editing, and nuclease editing, in virtually any cell type or organisms...

...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools...the gRNA in living cells. Broccoli aptamers are nucleic acid sequences that bind to the small molecule,...

...contain other cellular molecules such as proteins or nucleic acids. This conjunction of vectors and exosome ...contain cellular macromolecules (e.g. proteins, nucleic acids) which possibly improve transduction properties...

...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

... JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI) are capable of cleaving ...manufacturers make High Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid...

...transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (...

...biorender.com. ITR structure  WT ITRs are symmetrical 145-nucleotide sequences that flank the ends of the single stranded...Figure 2). Additionally, the ITR contains a 4-nucleotide Rep binding element (RBE) that serves as a binding...

...domain of the Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed...game of telephone, until the message reaches the nucleus where gene expression changes lead to cell proliferation...

...to prevent the incorporation of non-specific nucleotides into the dsRNA leading to increased RNAi efficiency... Find the T444T plasmid here! Strum A, et al. Nucleic Acids Research. 2018 PubMed PMID: 29924347 Faster...

...prevent them from being degraded by restriction endonucleases. A given prokaryote typically has genes encoding...groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same DNA sequence...

.... To make a point mutation, you design two oligonucleotide primers with the desired mutation, then use...REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion size, primer...

...are essential to the biotech startup puzzle: Nucleation of the team. The team is key, and the initial...team is the cornerstone of it all. Without a good nucleus, the company won’t go anywhere. The team should...

...we would be remiss not to at least mention. Oligonucleotides allow for sensitive detection of low abundance...multiplexing potential. You will find antibody-oligonucleotide conjugates used in assays like proximity ligation...

...underwent repair not from the single-stranded oligonucleotide (ssODN) template supplied by Ma et al., but...allele rather than the supplied single-stranded oligonucleotide (ssODN) template. Embryos also underwent editing...

...phosphorylated and red fluorescence can be found in the nucleus. For testing kinase inhibitors, psMEK1 and ERK ...downstream target ERK, the KTR remained in the nucleus. But if the inhibitor targeted upstream members...

...mutations, frame shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements... reference; and identifying mixed plasmid and nucleotide populations. All of these topics require additional...

... than the more efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR...codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids...