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  1. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...contains the signal peptide of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag...

  3. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...surface, dump gating is a useful way to get rid of undesired cell populations. Dump gating uses the same color...

  4. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...interest, and a donor DNA molecule containing your desired edit are all you need to generate a knock-in. ...

  5. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...if I had planned my day extensively, my lack of desire to go sit in the tissue culture hood may have prevented...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...response element (RRE), an internal promoter, and the desired gene (transfer vector plasmid), 2) the HIV-1 genome...

  14. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...contains both the rare off target event and the desired edit is low. Therefore, unbiased sequencing may...

  17. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...cell that gets the plasmid contains all of the desired gRNAs. This increases the chance that all the edits...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...figures were adapted from this reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for...
Showing: 161 - 180 of 181 results