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We narrowed to 520 results for: Set

Showing: 161 - 180 of 520 results
  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ...library that contains up-to-date information and data-sets, as well as an interactive gRNA viewer. The Moffat...address this problem, Csaba Pál’s lab has created a set of vectors that allows one to use the MAGE method...method in unmodified bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses the λ Red recombinase... kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository. The... unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement unused auxotrophies...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...
  2. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... go over the details step-by-step. 1. Digestion Set up restriction digests for your insert (or donor ...of recipient plasmid to insert. It is critical to set up a negative control ligation reaction with no insert...
  3. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    Published
    Sept. 16, 2025, 1:15 p.m.
    ...machine learning approach, the lab proposed multiple sets of Cas9 mutations that conferred new PAM preferences...mutations produced efficient editors, the Kleinstiver set performed best when paired with the V106W mutation...
  4. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ...extracted, sectioned, and imaged, with all image sets available at the Allen Institute’s Genetic Tools...Enhancer Collection is a quick guide to a smaller set of tried-and-true winners, containing enhancers with...; font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9...
  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...Goedhart and colleagues in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1...controlled phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and...
  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...specifically developed to thrive in laboratory settings. One of its most attractive features is its stability...easily maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms...
  7. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...in leukemia, the Lowe lab was able to identify a set of mutations that made cancer cells more resistant...systems check out Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally ...
  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...approaches to visualize bacteria in an educational setting. The Symposium on ‘Creative Approaches to Presenting...
  9. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ... part of my job, I travel around the country and set up meetings with PIs to discuss the benefits of depositing...
  10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...researchers are eager to apply it in a clinical setting. CRISPR is often used for animal germline modification...to deliver via gene therapy, so researchers have set their sights on another approach. Since shorter forms...
  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...fusion protein allows for LSD1 targeting to a wider set of genomic targets. These two vectors are part of...may make sense to try multiple CRISPR tools when setting up your experimental system. Let us know about ...
  12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...and Ioannidis 2005) and changing an experimental set-up partway through an experiment in an attempt to...a journal prior to performing the experiment (or set of experiments), the design would be reviewed, and...biological researchers deal with larger and larger datasets and use computer based analysis techniques, an...
  13. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... produce a BRET probe for imaging. These authors set out to design a better fluorescent protein that could...could be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...impractical or infeasible for certain experimental setups. The brightness of NLuc has caught the attention...
Showing: 161 - 180 of 520 results