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We narrowed to 608 results for: Sis;

Showing: 161 - 180 of 608 results

  1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...plants, and agricultural crops in particular, resistant to disease. They also devise biosensors designed... developed mostly with model plants in mind, Arabidopsis and Nicotiana benthamiana. N. benthamiana is ...Universities sign up and in the first year there is an analysis of women in faculty, how they are doing. They ...Plant SynBio Reading: Quick, Versatile Plant Transgenesis with GreenGate Plasmids  ...

  2. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...the alignment at a more distal amino acid. We consistently run our blastx searches with the “Low complexity...recommendation is not infallible, we have found it saves analysis time to remove this default setting. Timesaving...Depending on your sequencing result and desired analysis, BLAST may not always be your optimal choice. ...plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid Guide ...

  3. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... rare genetic eye disease, Leber’s congenital amaurosis. This is the first of what I, scientists, doctors...disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV gene therapy...childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 ... time and place. But it is the hard work and persistence of a dedicated team of scientists led by Dr. ...

  4. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...specific genomic region using the CRISPR system, consisting of dCas9 and targeting gRNA, to identify molecular...vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic ...Yuno, and Hodaka Fujii. "An enChIP system for the analysis of bacterial genome functions." BMC research notes...expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells 23.4 (2018): 318-325. PubMed ...

  5. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...primarily European reference genome could affect their analysis. Their work revealed that cell lines from individuals...information, Misek notes that in theory, if the DepMap analysis had an equal representation of every ancestry ...descent.Correcting the bias  While their thorough analysis uncovered some concerning biases within widely... team at Broad, they were able to change their analysis to use a corrected genome map. Now, any data that...

  6. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...bacterial resistance marker used to grow plasmid containing bacterial cultures Bacterial Resistance: Use this...terms with gene and guide RNA / shRNA inserts Resistance Marker: This indicates the selectable marker ... this to choose the antibiotic resistance gene(s) used for selection in bacteria. Has Viral Service?: ...

  7. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...across oceans and continents. Each deposit may consist of anywhere from one to hundreds of samples, with...plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves good ...your lab ensure that sample ID’s are unique, consistently labeled, and that numbers are not switched around... with plasmid transformation, preparation, and analysis from our protocols and molecular biology reference...

  8. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...purification with glutathione, can also protect against proteolysis. That’s one form of instability. Prokaryotes ...purification with glutathione; protects against proteolysis, but may reduce solubililty HA YPYDVPDYA or...EINAPTDGKV EKVLVKERDA VQGGQGLIKI GVHHHHHH  9 Combo Consists of a bacterially derived in-vivo biotinylation...KEFLDANLAG SGSGHMHHHH HHSSGLVPRG  12 Solubililty Assists in proper folding V5 GKPIPNPLLGLDST   1.4 Affinity...

  9. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...1]; and 2) the potential for oncogenesis through insertional mutagenesis. These risks are largely based...differ in two ways. Firstly, 2nd generation systems consist of an envelope plasmid and a packaging plasmid ...the deletion reduces the risk of insertional mutagenesis by limiting the interaction between viral LTR...

  10. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...transfection. PCR products are analyzed by gel electrophoresis to ensure the correct banding pattern and sizes...

  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...other cell lines, or infected personnel and can persist undetected; unlike infections with larger microbes...al. found that lentivirus produced at pH 6 are resistant to multiple freeze thaw cycles. Consequently, ... to ensure the best possible transduction. Be consistent Once your lab establishes the best conditions...for your cell lines and virus properly and be consistent when following protocols. Need Virus? Check out...

  12. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...rudimentary, but before you do any type of behavior analysis, simply take a few animals and stimulate them ...optogenetics experiment is contained within the hypothesis you are testing. In other words, your optogenetic...explained primarily in two different ways 1) your hypothesis is wrong or 2) there is some problem with the...optogenetic tools derived from direct comparative analysis of microbial opsins." Nature methods 9.2 (2012...

  13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...contributed by guest blogger Joachim Goedart, an assistant professor at the Section of Molecular Cytology...coded by the multiple cloning site and therefore consist of random amino acid sequences or (ii) designed... an inert, unstructured peptide and therefore consisted of glycines, serines and other small, non-aliphatic...blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

  14. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...loss. Ci Ji Lim’s lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that... by helical LEAs. C-E) cryo-EM single-particle analysis of PRC2 with RvLEAMshort (1:6 molar ratio) with...s other recent deposit builds on Prime-editing-Assisted Site-Specific Integrase Gene Editing (PASSIGE)...CRISPRoff system — a programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...of WT and edited cell lines. Then decomposition analysis is performed yielding a graph of edits in the ... Fig. 3: Restriction enzyme screening for resistivity to digestion, indicating targeted edits. ...solely for restriction enzyme sensitivity or resistivity, and thus be a marker for edited cells. As with...Software such as CRISPResso can help with the data analysis. If NGS is not an option, you can still detect...

  16. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... are essential for processes such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction...Kresege et al., 2004). Avidin is a glycoprotein that consists of four identical subunits that can each bind ...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...

  17. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...off-target editing. In order for the therapy to persist over time, stem cell editing would be highly desirable...improved, with vastly reduced inflammation and necrosis compared to unedited animals. In grip strength...approach, increasing the possibility that edits will persist long-term. In accordance with this result, Nelson...CRISPR Could Be Used to Treat Cataracts and Cystic Fibrosis Read How Homology Directed Repair is Used to Edit...

  18. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ..., it would be unwise to make conclusions on the basis of the activity of a single gRNA, and thus diversity...on careful experimental design, execution, and analysis. Many thanks to our guest blogger John Doench....2017.97 Hanna RE, Doench JG (2020) Design and analysis of CRISPR–Cas experiments. Nat Biotechnol 38:813...https://doi.org/10.1038/s41576-018-0059-1 Veres A, Gosis BS, Ding Q, Collins R, Ragavendran A, Brand H, Erdin...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...systems. Binary systems are genetic tools that consist of two parts, both of which must be present for...allows them to be used together for complex genetic analysis in Drosophila or for precise control over multiple...Desplan. “Power tools for gene expression and clonal analysis in Drosophila.” Nat Methods 9(1) (2011): 47-55...transgene expression, lineage tracing, and mosaic analysis.” Cell 141(3) (2010): 536-48. PubMed PMID: 20434990...

  20. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...the Moloney Murine leukemia virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing...generation. The 1st generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector...Efficient gene transfer Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human... from one batch to another. For experimental consistency it’s a good idea to purify and titer your virus...
Showing: 161 - 180 of 608 results