We narrowed to 542 results for: TIL
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TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....Addgene plays in accelerating research. Yet this was still not enough to ensure we could keep up with demand...organizations to request support for our operations until our normal work resumed. Addgene distributes and...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m.... to visualize individual/non-overlapping cells. Until now, tools for doing this were labor-intensive and...intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell types and... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....injection. How long post-injection did you wait until performing the experiment? What detection methods...data. Please note that the data hub is currently still in beta version, so we’re always looking for ways... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m.... described more than 60 years ago, but there is still no treatment available. Aguilar is a basic cell ...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m.... an improved apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11...Drosophila, an observation that has been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is ... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m.... well-designed fragment overlap sections, while still creating scarless plasmid inserts. Want to Learn...digestion, and used directly in DNA assembly will still yield scarless assemblies. Overview of NEBuilder... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m.... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. A. ... A. Elliott, A. H. Brand, The GAL4 System : A Versatile Toolkit for Gene Expression in Drosophila, 3, ... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....many more viral particles to your solution while still using a volume that’s compatible with your experimental... Control: Going the Extra Mile with NGS AAV: Versatile Viral Tool for Gene Expression in Mammals Additional... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m.... prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer science...developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m....libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution...library, this is less of a concern, but Addgene still recommends plating the transformation mixture on... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....do we get where we want to be? The part that is still missing is a clear alignment of incentives. Right...is clearly room to do more. If we could really instill a culture of rewarding good scientific citizenship... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....gets a chance to talk and less vocal team members still have their voices heard. Here's a template for team...other’s projects. For all-company meetings, monthly still works best. Figure out what works for you for each... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....miRNA regulatory process. This early research is still relevant to his work at the Broad Institute today...asking biological questions. Doench loves that he still gets to see data as it’s coming out and says “That... -
Resume Writing for Non-academic Science Careers
TypeBlog PostPublishedDec. 18, 2014, 4:06 p.m....your career planning early, start now Don't wait until the day you defend to start thinking about your ... yourself a sense of what to expect. If you are still debating whether to stay in academia or not, it ... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....integration site. The integrated phage remains dormant until it is triggered to enter the lytic cycle. During... bacterium contains a DNA sequence called the Fertility factor (F-factor). The F-factor is found on an... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m.... networks of specific proteins in living cells. Until recently, the enzymes most frequently used for PL...paper, they describe TurboID and miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....we can’t forget that those other components are still there in real life. Certainly referring to any ...-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. J. ... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....the surface of the bead, suggesting that OptoNBs still bound their target protein even when stuck to the...This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene expression...