We narrowed to 546 results for: TIL;
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TypeBlog PostPublishedApril 30, 2024, 1:15 p.m.... Molecular basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into...are non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform...
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Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known edible relatives...include growing plants require pots, trays, soil, fertilizer, seeds and a growth space with fluorescent lights... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments. Read more...and to find a video of them in action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....researchers lamenting the viral production process while still ordering viral vectors. Virus production can be ...Beginners Lentiviral Vector Uses and Overview AAV: A Versatile Tool for Gene Expression in Mammals Additional... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m.... described more than 60 years ago, but there is still no treatment available. Aguilar is a basic cell ...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....do we get where we want to be? The part that is still missing is a clear alignment of incentives. Right...is clearly room to do more. If we could really instill a culture of rewarding good scientific citizenship... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m.... an improved apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11...Drosophila, an observation that has been difficult until now (Zhang et al., 2019). Conclusion FlipGFP is ... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....many more viral particles to your solution while still using a volume that’s compatible with your experimental... Control: Going the Extra Mile with NGS AAV: Versatile Viral Tool for Gene Expression in Mammals Additional... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m.... prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer science...developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog PostPublishedOct. 26, 2017, 1:59 p.m....libraries are some of Addgene’s most exciting and versatile offerings! We recently re-amplified our distribution...library, this is less of a concern, but Addgene still recommends plating the transformation mixture on... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m.... well-designed fragment overlap sections, while still creating scarless plasmid inserts. Want to Learn...digestion, and used directly in DNA assembly will still yield scarless assemblies. Overview of NEBuilder... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m.... convince him to use Twitter @jonchowphd but he still reads “#” as a “pound symbol”. References 1. A. ... A. Elliott, A. H. Brand, The GAL4 System : A Versatile Toolkit for Gene Expression in Drosophila, 3, ... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....gets a chance to talk and less vocal team members still have their voices heard. Here's a template for team...other’s projects. For all-company meetings, monthly still works best. Figure out what works for you for each... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....miRNA regulatory process. This early research is still relevant to his work at the Broad Institute today...asking biological questions. Doench loves that he still gets to see data as it’s coming out and says “That... -
Resume Writing for Non-academic Science Careers
TypeBlog PostPublishedDec. 18, 2014, 4:06 p.m....your career planning early, start now Don't wait until the day you defend to start thinking about your ... yourself a sense of what to expect. If you are still debating whether to stay in academia or not, it ... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....the surface of the bead, suggesting that OptoNBs still bound their target protein even when stuck to the...This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene expression... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....integration site. The integrated phage remains dormant until it is triggered to enter the lytic cycle. During... bacterium contains a DNA sequence called the Fertility factor (F-factor). The F-factor is found on an... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....protein:DNA complexes will remain bound to each other until you are ready to separate them. Most proteins are...This will keep the protein:DNA complexes together until you want to break them. Crosslinking is necessary... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....we can’t forget that those other components are still there in real life. Certainly referring to any ...-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents...