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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 434 results for: Trac;

Showing: 161 - 180 of 434 results

  1. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...being able to actually hold all those seemingly abstract biological reactions that I learned about from... Making proteins outside of a cell - cell free extracts Typically, if you want to make a protein – say...

  4. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...of that, perhaps you were involved in some extracurricular activities – did you volunteer to do science..., University of British Columbia. Originally on track for a research career, she transitioned into a communications...

  5. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...you’re within a year from the end of your PhD or contract, you should be starting to send out polite emails...can. You know you are in the zone when you lose track of time because you are engrossed in a task. Things...

  6. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...components. Transfer cPPT Central polypurine tract; recognition site for proviral DNA synthesis. Increases...transcription started by 5' LTR by the addition of a poly A tract just after the R sequence. This post was written...

  7. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists...people ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9:...gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA? Yes No dsDNA cleavage Blunt end 5' overhang...

  10. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...inventory management systems to enable efficient, tracked production and distribution of viral samples. The...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...tasks. EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...

  13. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro...and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits and...

  15. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion channel proteins, they are not always easy to track at the transcriptional or translational level. However...fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states (Matthew et al., 2018). These...

  16. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ... in each plasmid. Each gRNA combination can be tracked and quantitatively analyzed by sequencing its set... gRNA Read How Barcodes Can Be Used in Lineage Tracing Read about CRISPR/Optogentics Tools Resources ...

  17. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...through our deposit process, and then we carefully track samples through our lab using barcoded tubes and...Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View ...

  18. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... violet light and (2) exposure to increased intracellular calcium. It has been applied to mark active ...Whether you’re studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry...

  19. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...near point mutations to create 3-base (or longer) tracts of edited bases. DNA mismatch repair (MMR) is less... protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) can improve...
Showing: 161 - 180 of 434 results