We narrowed to 635 results for: Uty

...problem/task/situation, come up with potential solutions, and decide on a course of action that effectively...by paying attention to how your mentors reach solutions and ask them to explain their logic. Or you can... probably did not just automatically come to a solution. Consider the thoughts that went through your ... challenge yourself to think of more creative solutions. Ultimately, problem solving is less about technical...

...labs have recently published work that provides a solution to this problem. A miniature CRISPR-Cas system...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...mismatch repair (MMR) interfered with the desired resolution of prime editing intermediates. Building upon...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...

...membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the influenza...immunohistochemistry methods, these proteins are routinely fused to ‘tag’ or ‘label’ proteins to facilitate...nanogold particles, that allow a high-spatial resolution of the protein localization. Surprisingly our...

... Nobel Prize in Chemistry for his contributions to super-resolution light microscopy, comes from his recent...article “SRpHi ratiometric pH biosensors for super-resolution microscopy.” Endosomes are historically difficult... endosomes, the Hell lab designed their super-resolution pH indicators (SRpHi) biosensors to meet all ...

...concentration of the protein solution. During the experiment, the solution is diluted, complicating the...determine the capacity of homodimerization in aqueous solution. In cyto tests for aggregation To what extent ...the data presented in papers should be used with caution, since the difference between studies is substantial...

...worry about and that is something to recognize! Distributing plasmids, viral service, and more For the ...about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% every year...since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere can access plasmids...Addgene. There’s so much potential for service and contribution! Leadership On an organizational level, ...

...kinase activity can provide greater spatiotemporal resolution than pharmacological or genetic approaches, but...for creating optically controlled kinases. The Solution: In Zhou et al, the Lin lab present a generic ...domains on either side of the active site of a constitutively active MEK1. This photoswitchable MEK1 is called...Raf-MEK-ERK signaling pathway to demonstrate the utility of  photoswitchable kinases in vivo with C. elegans...

...it. This will help you understand what safety precautions to take, and if something does happen, ensure... retrovirus) and gamma retrovirus require more caution as they are integrating viruses – they physically... decontaminate in the waste itself, not in the solution that should be added. After decontamination, ... most common forms of lab accidents, so extra precaution should be used to reduce the risk of exposing...

...to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification...sequence discrepancies can be caught prior to distribution. Catching these errors prevents future experimental...can pass through QC and be made available for distribution. In these cases, the QC team leaves a note on...written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly...

...appreciate that you took the time to think of solutions, and will be more likely to view your proposals...viewpoint and brainstorm about mutually beneficial solutions Your supervisor might not agree with all of your...problem from their viewpoint, and brainstorm about solutions that will meet both of your needs. 6) Put important..., and discuss options for mutually beneficial solutions. If you package your challenges with your supervisor...

... the pharmaceutical industry for years. They have proven very difficult to target therapeutically due ... high-affinity binding targets for potential therapeutic agents. Nevertheless, a glimmer of light remains... important pathways. We invite feedback and contributions from all members of the research community as...

...potential gene drives should be conducted with extreme caution. Why does making transgenic organisms with Cas9...would find our work and consequently use these precautions in their own experiments2,3. While that didn'...blogger! An inventor of technologies that harness evolution, Kevin Esvelt studies ways of using molecular ...transparently.  For more information, visit www.sculptingevolution.org. References: Burt, A. Site-specific...

...engineered DNA systems capable of storing data and computing inside living cells. He's also a co-founder of...digital genetic switches that enable cellular computing. These logic gates built from transcriptors are... this way: "Addgene is one of the leaders in distributing plasmids amongst the academic community. On ...anticipate plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future...

... chemistry and evolution to illuminate biology and enable next-generation therapeutics. Prime editing,...noise to each of the terms which make up our distribution, resulting in the following hierarchical model...University. He is interested in using selection, evolution, and machine learning to solve difficult problems...

...there are benefits to optimized libraries that utilize only a few sgRNAs per gene. These libraries provide...., 2016, Sanson et al., 2018). These libraries utilize optimized sgRNAs that were designed based on the...while bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional...

...be easy to get caught up in the minutia of the workday, but that minutia gets much more manageable when...games. In previous years, this event was held at Executive Director Joanne Kamen’s home- a testament to the... the lab was such a master drywall hanger?  4. Utilize your office space too Balance point number 3 with...

...differently from you and you will arrive at unique solutions to the same problem. If your lab/company/team ...because they will fight more adamantly for their solution), selecting a devil’s advocate is a good option...timer for five minutes and come up with as many solutions as you can in that timeframe. Tell yourself they...slowly turn your unworkable idea into a viable solution.  Anticipate Change Just because a process is ...

...Liu lab recently used phage-assisted continuous evolution and protein engineering to generate seven new ...J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield compact, efficient...lab recently made two deposits that increase the utility of this technique.  By engineering a light-sensitive...antibodies in Addgene’s collection since we began distribution and has been verified to perform well in standard...

...molecules. A solution of a longer DNA fragment will contain fewer molecules than a solution of a shorter...written by Alfonso Timoneda, with significant contributions from Robert Hurt and Mohamed Soufi. So you’ve...(and therefore positive colonies) after each consecutive assembly and transformation round, due to the...

...the lab based team as part of their general job duties. Bigger companies have dedicated technical support...customers, internal resources and databases are scrutinized, and this information is enriched with your technical...discussions can often lead to otherwise unobtainable solutions. To some extent there is the need (or opportunity...problems are the most rewarding parts of the job. Solutions may also be achieved by simply replacing the product...