...co-founded by Kaspar, acquired by Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies...2013). Mutations that affect this interaction to varying degrees determine the clinical severity of different...et al., 2019). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu,...

...biology for over three decades. Widely used across various research applications, they have become indispensable...manipulating cells and organisms. They can be used for a variety of research purposes, like creating stable cell...Leavitt et al., 1996), and since then, numerous variants have been developed with different mechanisms ...PMC2496964 Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996). Human immunodeficiency virus type...

... other people for a variety of projects. I was therefore forced to learn a variety of ways to manage my...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...of my favorite podcasts. JUST GET STARTED - One Harvard Business Review article on procrastination recommends...

...single nucleotide variations. Also, FnCas9 cleaves in a range of temperatures varying between 10-50°C. ...similar to the SHERLOCK diagnostic test, this test varies in the sequence of steps and the Cas protein used...Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12...

...Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized...fluorescent aptamers Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described...short non-coding RNAs (Autour et al., 2018). Three variants of Mango, coined Mango II, III, and IV, were then...high binding affinity to TO1-biotin. Two of these variants exhibit increased brightness compared to Mango...

...state of research became more clear throughout the various sessions of the symposium. Structural problems ...and interact more with people who have taken a variety of career paths and should be able to take steps...we try to do our part in boosting awareness of various career opportunities through our career, networking... in Publishing Panel brought in editors from a variety of publications as well as young PIs and all gave...

...Presentation Once you’ve captured your images, various software programs will allow you to align the images...controls! The necessary control experiments will vary depending on your specific experimental design. ...possibilities, and there are always new and improved variations you could try. We hope this post helped you start... H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M., & Weissgerber...

... in which the plasmid was observed to exist in various multimeric forms (e.g. concatemers, dimers, trimers...detection and can provide data on the quantity of various topologies and multimers present within a plasmid...bacterial population as a mixture of monomers and various forms of multimers (Summers & Sharratt, 1984; Summers...Berg CM, Liu L, Coon M, Strausbaugh LD, Gray P, Vartak NB, Brown M, Talbot D, Berg DE. pBR322-derived ...

...different sectors. Luckily, I have worked for a variety of different organizations, so I tend to provide...commercial company; the pay was good and there was a variety of work. The work was very scheduled and it was...solve experiments over the phone or explain how various plasmids work. Most people who call or email me...your own work schedule.  I really love my highly varied role, it has allowed me to travel and to share ...

... 101: Validating Your Genome Edit, there are a variety of options for CRISPR genotyping. The most common... sensitivity Sanger Sequencing Provided 20% (variable) Sequence-level data Low-throughput, inappropriate... Targeted Amplicon Sequencing Provided 0.01% (variable) Sequence-level data, extremely sensitive Do not...sequencing: a comparison of methodologies for KRAS variant detection." Biotechniques 53.3 (2012): 182-188....

...contributed by Doug Richardson, Director of the Harvard Center for Biological Imaging and a Lecturer on...on Molecular and Cellular Biology at Harvard University. No matter whether you are a sports photographer...Richardson! Doug Richardson is the Director of the Harvard Center for Biological Imaging and a Lecturer on...on Molecular and Cellular Biology at Harvard University.     Additional Resources Online Microscopy Primer...

...Mani and Amy Gilson from Science in the News at Harvard University. Effective science communication is ...Amy Gilson. Vini Mani is a graduate student at Harvard University, former Co-director of Science in the...     Amy Gilson recently received her PhD from Harvard University, is a former Co-director of Science ...

...–10 mg *Can vary from kit to kit, check the manufacturer’s documentation **Varies by plasmid copy ...control information, but the level of detail can vary. If you’re new to using purification kits, or if...3 below contains details regarding kits from a variety of manufacturers. It also includes a rough comparison...

...constructs improved editing in various cell types, although efficacy varied among target sequences. The ...they expanded CGBE target scope by creating BE variants with other Cas9s that recognize different PAMs...capable of predicting editing outcomes of their various CGBEs at different target sequences and in different...

...confirming its sequence. This region of the protein varies between Cas9 enzymes from different species, explaining...can’t bind to this duplex by its base pairs; those vary from target to target. Instead, both lobes bind ... loop to produce SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo...Components of CRISPR  xCas9: Engineering a CRISPR Variant with PAM Flexibility Cas9 vs the Other Cases Further...

...have a variety of downstream effects including possibly decreasing the number of postdocs various academic...Program in Biological and Biomedical Sciences at Harvard University. Ben and Niroshi have tons of great ...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

... Biological and Biomedical Sciences program at Harvard University to get the scoop on the ups and down...candidate in the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...of the PhD program in Genetics and Genomics at Harvard Medical School, and is currently a fellow in clinical...

...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...describes how to target and use FRET flurophores for a variety of applications. While editing this post, I learned...

... Biological and Biomedical Sciences program at Harvard University and pick their brains on how they've...candidate in the Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation...of the PhD program in Genetics and Genomics at Harvard Medical School, and is currently a fellow in clinical...