We narrowed to 176 results for: acr.2

... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...Fluorescent Proteins: Multiplexing and Optogenetics across Scales. Trends Biotechnol 36, 1230-1243 (2018)....near-infrared fluorescent proteins for multicolor imaging across scales. Nat Commun 11, 239 (2020). Oliinyk, O.S...

...) 12 (>100) xCas9(3.7)-ABE 69 (1.4) 43 (2) 21 (3) Not examined 16 (>100) Average percent...off-target activity. Surprisingly, off-target effects across 5 gRNAs with NGG PAM sequences were actually lower...

...PMID: 25538079. PubMed Central PMCID: PMC4353587. 2. Stephen, Andrew G., et al. "Dragging ras back in ... in the kit, the transcript most commonly found across all TCGA tumor samples is represented, making the...

... replication competent virus [usually HIV-1]; and 2) the potential for oncogenesis through insertional...screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources on Addgene.org Addgene's most...

...that numbers are not switched around or misread.  2. Track the container - It is just as important to ...weeks in transit and some have been transported across oceans and continents. Each deposit may consist...

...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...passion  Skills such as research, collaboration across multiple disciplines, writing, project management...

...externally fertilized, transparent progeny (Figure 2). These features give it a leg up (or a fin up?) over...1,500 zebrafish mutants that are still used in labs across the globe (Nüsslein-Volhard et al., 2012). One ...

...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...centrifuging. Spinfection transduction efficiency can vary across cell types and generally requires optimization ...

...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...portable method for bacterial genome engineering across multiple species Recent discoveries in the fields...

...positive cells (Martin et al., 2019). Figure 2: Editing efficiencies using base editing reporters... sorting using FACS which could limit their use across different cell types and systems. To introduce ...

...community is active, engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of...organize and easily find the resources that I come across on the internet or receive via email. The cool ...

... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...delivering two halves of a repair template spread across two AAVs, it is possible sequentially repair a ...

...Postdoctoral Associations around Boston - From Panel Session 2: Defining the Postdoc: An Institutional Perspective...was clear that, even though postdoc associations across different universities are trying to compile more...

... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water... determination on magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499...

...improvement in muscle physiological performance.  2. Trans-splicing Overview: For trans-plicing, splice...transgene.  Drawbacks:  Trans-splicing occurs across the ITR junction, and this is likely a rate limiting...

...module_attribute "schema_version" is_json="true" %}{% raw %}2{% endraw %}{% end_module_attribute %}{% module_attribute...activity of a single gRNA, and thus diversity of gRNAs across a gene should be examined whenever possible when...