We narrowed to 171 results for: acr.2

1. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...that numbers are not switched around or misread.  2. Track the container - It is just as important to ...weeks in transit and some have been transported across oceans and continents. Each deposit may consist...

2. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   Published

   Aug. 20, 2015, 2:30 p.m.

   ...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...passion  Skills such as research, collaboration across multiple disciplines, writing, project management...

3. Viral Vectors 101: Preparing Pooled Libraries

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   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...centrifuging. Spinfection transduction efficiency can vary across cell types and generally requires optimization ...

4. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   Published

   Aug. 13, 2019, 12:58 p.m.

   ...externally fertilized, transparent progeny (Figure 2). These features give it a leg up (or a fin up?) over...1,500 zebrafish mutants that are still used in labs across the globe (Nüsslein-Volhard et al., 2012). One ...

5. 27 Hot Plasmids from 2016

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   Blog Post

   Published

   Dec. 22, 2016, 3:03 p.m.

   ...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...portable method for bacterial genome engineering across multiple species Recent discoveries in the fields...

6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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   Blog Post

   Published

   July 7, 2020, 1:15 p.m.

   ...positive cells (Martin et al., 2019). Figure 2: Editing efficiencies using base editing reporters... sorting using FACS which could limit their use across different cell types and systems. To introduce ...

7. 5 Reasons to Use Reddit for Science Communication

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   Blog Post

   Published

   Oct. 20, 2016, 2:30 p.m.

   ...community is active, engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of...organize and easily find the resources that I come across on the internet or receive via email. The cool ...

8. Adeno-associated Viruses (AAVs) for Genome Editing

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   Blog Post

   Published

   March 27, 2018, 1:32 p.m.

   ... PMID: 9537413. PubMed Central PMCID: PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating...delivering two halves of a repair template spread across two AAVs, it is possible sequentially repair a ...

9. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...Postdoctoral Associations around Boston - From Panel Session 2: Defining the Postdoc: An Institutional Perspective...was clear that, even though postdoc associations across different universities are trying to compile more...

10. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water... determination on magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499...

11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...improvement in muscle physiological performance.  2. Trans-splicing Overview: For trans-plicing, splice...transgene.  Drawbacks:  Trans-splicing occurs across the ITR junction, and this is likely a rate limiting...