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...announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries...reagents available to the research community through Addgene and look forward to another great year of sharing...PubMed Central PMCID: PMC4486245. Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral Guide Pages Find ...

...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube! In this series...

...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology.... coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters just ...microbial technology, sign up to write about it on the Addgene blog. ...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...organisms we should include by emailing us at help@addgene.org. If you are looking for plasmids used in E. ...

...blog@addgene.org.  Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...decided to share some of our tips in our new series: Addgene Lab Tips! In each short episode of this series...Science: We Share Science Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol...

...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...making this transition. In this installment of the Addgene career guide, we’ll think about what causes this...job candidate for a senior scientist position at Addgene, the skeptical applicant asked if she would partake...of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list of things...the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is always ...someone who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly...fix them, and take a look at these tips from the Addgene blog. You’ll have the opportunity to discover...

... available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...been functionally tested by Ginkgo Bioworks or Addgene. If you used plasmids from this collection in your...cerevisiae expression vectors are available at Addgene. A set of mammalian expression vectors containing...COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received Ginkgo’s first... our standard full plasmid sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids...screening and the use of high throughput platforms, Addgene and Ginkgo are striking a balance by modifying ...978-1-59745-181-9_23 Additional resources on the Addgene blog Read all of our COVID-19 blog posts Learn...

...Introduction to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the construction...that will be the first antibodies distributed by Addgene.  The plasmids used to generate these antibodies...sequence. These plasmids have been deposited with Addgene for distribution to the scientific community with...affinity reagent tools to include nanobodies. At Addgene, we are excited to be part of this initiative and...References and Resources Additional Resources on the Addgene blog:  Plasmid-Based Recombinant Antibodies  Plasmids...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids... addgene podcast...synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology ...

... field. Resources at Addgene Browse Addgene's RNAi resources Browse Addgene's CRISPR resources Read ...countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne...conference invited me to attend and speak about Addgene’s experience helping scientists share plasmids, ... talk to so many scientists who were potential Addgene requestors and depositors. China is in one of the...conference was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

...contain thousands or millions of different books. Addgene’s pooled libraries are a little different: each ...are currently 390 pooled libraries available at Addgene, with more added to the collection all the time...technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also includes a base editing sensor...we whetted your appetite for pooled libraries? Addgene’s pooled library collection page features hundreds...are offered. Figure 4: Screenshot of the Addgene website. Let us know which pooled libraries...comments! Resources Additional resources on the Addgene blog Viral Vectors 101: Preparing Pooled Libraries... Pooled Library Amplifications Resources on Addgene.org Browse all pooled libraries Guide to Plasmid...

... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...but browsing the 49 new preps available in the Addgene viral vector repository with a nice cup of hot ...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

... autophagy biosensors currently available from Addgene. Rosella autophagy biosensor Rosella is a dual-...-targeted and mitochondria-targeted Rosella at Addgene! Keima autophagy biosensor Keima is a mammalian...Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy... red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases... flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo...PMCID: PMC5618514. Additional resources on the Addgene blog Beware of fluorescent protein oligomerization... sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein plasmids and resources Fluorescent...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Plasmid Rap Plasmid rap video made with support from Addgene.      Check out more science education tools...Additional Resources on the Addgene Blog Get Advice on How to Build a Grad Student Community Check out All...Plasmids 101 Series Additional Resources on Addgene.org Check out Our Protocol Pages for New Protocol...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing open source sharing...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...

...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook...end goal. CasPEDIA also features useful links to Addgene resources throughout the site. In the Experimental...each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that enzyme... from the article if they're available through Addgene. So whether you're looking for the exact Cas9 plasmid... or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered!     ... eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment...

...NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's most popular retroviral...retroviral plasmids Addgene's collection of AAV plasmids Addgene’s collection of adenovirus plasmids Resources...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...components of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for ...Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral Packaging Plasmids...

...they grow. The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral...components over the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes...2A-Puro (PX459) V2.0 was made available through Addgene in 2015, making this the most recently available...fact: published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd...shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more...projects in the years to come.  Resources on the Addgene blog Browse our CRISPR posts Read our viral vector...posts Read our lentivirus guide Resources on the Addgene website Browse our CRISPR collection Find plasmids...

... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence... plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we distribute...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...plasmid you are working with, but, in most cases at Addgene, we check what we call the “insert,” (the gene ...comments section below.  Additional Resources on the Addgene Blog Get 6 Tips for Analyzing and Troubleshooting...