We narrowed to 319 results for: bre

1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

   Type

   Blog Post

   Published

   Feb. 18, 2015, 3:09 p.m.

   ...Cas9, we began to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly...

2. Three Tips to Organize Your Lab Notebooks in the New Year

   Type

   Blog Post

   Published

   Jan. 2, 2014, 3:44 p.m.

   ... your first day back in the lab after a holiday break filled with family, food, and travel, and you can...

3. Tag Your Favorite Yeast Genes with Ease

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   Blog Post

   Published

   Nov. 19, 2013, 2:37 p.m.

   ...repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of this ...

4. Supporting Rare Disease Research with BeHeard 2017

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   Blog Post

   Published

   July 20, 2017, 2:30 p.m.

   ...We often hear about breakthroughs in diabetes and lymphoma research or the many different ways you can...

5. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

   Type

   Blog Post

   Published

   March 10, 2016, 3:30 p.m.

   ...on the right side of the search page. A great breakdown of our search functionality can be found in the...

6. pSiM24: Simplifying Plant Genetic Engineering

   Type

   Blog Post

   Published

   Sept. 29, 2015, 2:30 p.m.

   ...important foundation for life on Earth. Selective breeding methods have shaped the plants that we grow and...

7. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

   Type

   Blog Post

   Published

   Sept. 15, 2014, 6:05 p.m.

   ...emphasized the potential of this extraordinary breakthrough. In fact, the panelists agreed that, for example...

8. London Calling - Addgene’s New Europe Office in London

   Type

   Blog Post

   Published

   June 10, 2014, 3:20 p.m.

   ...using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression...

9. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...create a single-strand rather than a double-strand break, and when used with two adjacent gRNAs, can lower...

10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type

    Blog Post

    Published

    Dec. 12, 2017, 3:13 p.m.

    ...>G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids for...

11. Tips for Improving Your Next Manuscript

    Type

    Blog Post

    Published

    Nov. 22, 2016, 3:30 p.m.

    ... The most beneficial aspect of this course was breaking up into focus groups with a facilitator. In the...

12. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    Published

    June 11, 2020, 1:34 p.m.

    ...Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the limits of light delivery...

13. CRISPR 101: Drug discovery and CRISPR

    Type

    Blog Post

    Published

    May 4, 2023, 1 p.m.

    ...you identified gene target X for BRCA-deficient breast cancer and identified several candidate inhibitors...

14. An Addgene Summer Internship

    Type

    Blog Post

    Published

    Aug. 8, 2024, 7:22 p.m.

    ...week at Addgene. This report shared my work by breaking down the process of each day, my background, observations...

15. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type

    Blog Post

    Published

    Oct. 3, 2017, 12:11 p.m.

    ...’s biggest piece of advice for those looking to break into the field of science communications-- Be able...

16. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    Published

    June 30, 2015, 2 p.m.

    ...cell.4.) The transposase creates a double strand break in the DNA and allows the tranposon to integrate...

17. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type

    Blog Post

    Published

    Jan. 15, 2015, 1:50 p.m.

    ...meeting, and its history of code making and code breaking is wonderfully analogous to genetic circuits discussed...

18. Advice for Choosing a Research Project

    Type

    Blog Post

    Published

    April 17, 2014, 2:45 p.m.

    ...topic that “looks at the complexity of biology and break apart a piece that can address a topic in a mechanistic...

19. CRISPR/Cas9 FAQs Answered!

    Type

    Blog Post

    Published

    March 13, 2014, 4:08 p.m.

    ...doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does. There are a few references...

20. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...much as with technical knowledge. I leverage a breadth of knowledge to put diverse aspects of bioscience...