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Showing: 161 - 180 of 308 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...Several companies supply gRNA sequences, such as GenScript, ThermoFisher Scientific and OriGene, or you can... mice, you can clone the PCR products into a pBluescript cloning vector and sequence the resulting plasmid...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...) to remove mutated exons from the dystrophin transcript. Unfortunately, the oligonucleotides only modestly...in one experiment, they found the exon-skipped transcript constituted 59% of total dystrophin mRNA, similar...

  5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...sensors do not necessarily have to be based on transcriptional regulation, as long as they can control recombinase...recombinase activity. For example, post-transcriptional recombinase activation via RNA toehold riboregulation...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...sequence prior to an ATG start codon to prevent transcription in cells where the recombinase was not present... transgene into the correct orientation for transcription. Upon recombination one of lox2272 sites will...

  7. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... ready for experimentation. In 2014, several manuscripts published in Genetics detailed approaches in ...plasmid targeting vectors that encode the gRNA transcript and the repair template of your choosing. Tools...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...

  9. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas...

  10. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...of our Data Team, “Our patchwork collection of scripts evolved into a single, organized pipeline and accelerated...

  12. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day...

  14. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...institution. Requesters will need to provide a brief description of the applicable research project when purchasing...

  15. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ... be a few tips for scientific communication (manuscripts, talks, etc…!)  Deep Dives You probably are familiar...

  17. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...microbial pigment. Check out the hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com...

  20. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ... The new search bar.   Pro tip! You can use a manuscript title to search for plasmids from a specific ...
Showing: 161 - 180 of 308 results