We narrowed to 322 results for: cript

...the library sample, and any other information or scripts provided by the depositing lab for data analysis...2017). Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc, 12(4), 828...

...synthesis companies, such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So...ATUM DNA2.0  IDT Synthetic gene design made easy GenScript- GenSmart Codon Optimization References Athey...

...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert amplified...

... and inhibit its own transcription. As copy number increases, RepA transcription is repressed and replication...

...antibiotic’, very often we immediately imagine a prescription for a bacterial infection. While this post will...development of resistance include (Figure 1): Over-prescription of antibiotics (for example, giving antibiotics...

...Several companies supply gRNA sequences, such as GenScript, ThermoFisher Scientific and OriGene, or you can... mice, you can clone the PCR products into a pBluescript cloning vector and sequence the resulting plasmid...

...frames downstream of the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002... mRNA Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–...

...) to remove mutated exons from the dystrophin transcript. Unfortunately, the oligonucleotides only modestly...in one experiment, they found the exon-skipped transcript constituted 59% of total dystrophin mRNA, similar...

...reporter to show that the mutants abolished transcriptional activity, even in the presence of endogenous...carried by extracellular vesicles (EVs), edited transcripts could endow neighboring cells with pro-oncogenic...

...perform in vitro transcription, and use the IVT product as a template for reverse transcription to create your...

...sensors do not necessarily have to be based on transcriptional regulation, as long as they can control recombinase...recombinase activity. For example, post-transcriptional recombinase activation via RNA toehold riboregulation...

...sequence prior to an ATG start codon to prevent transcription in cells where the recombinase was not present... transgene into the correct orientation for transcription. Upon recombination one of lox2272 sites will...

... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...

... ready for experimentation. In 2014, several manuscripts published in Genetics detailed approaches in ...plasmid targeting vectors that encode the gRNA transcript and the repair template of your choosing. Tools...

...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas...

...a trade-off: overextension past the reverse transcriptase (RT) template, resulting in unintended insertions... implicate specific residues in the reverse transcriptase polymerase domain as likely determinants of ...

...of our Data Team, “Our patchwork collection of scripts evolved into a single, organized pipeline and accelerated...

...on the B. subtilis libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming efficiency...

...Noninvasive assessment of gut function using transcriptional recording sentinel cells! 🎵 On the 11th day...

...word of mouth and personal networking!) and the manuscript review/submission process at JEI. Chapter 3 -...