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Showing: 161 - 180 of 759 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...induced pluripotent stem cells (iPSCs) capable of forming nearly all cell types in the body from otherwise... battery of questions regarding topics such as informed consent (How do we prevent stem cell lines from...meeting shared plenty of details, but they also uniformly stepped back to give a big picture perspective...

  2. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...medium, without which the cells cannot reproduce to form colonies (used to complement auxotrophic cell lines...produces pigment from x-gal (included in the transformation plate medium), turning the bacterial colony...for blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies...components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may contain genes that...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...revision before acceptance. F1000Research uses a novel form of peer review that occurs post-publication and ... required information is provided. The clearer you write your paper and the more information you provide...journal’s coverage. These guidelines also provide information on a journal’s house style and the things you...you need to include in your manuscript. What information do I need to include in my paper? More and more...more journals are asking for detailed information on lab resources, such as catalogue numbers and manufacturers...joined a pilot project from the Neuroscience Information Framework (NIF) called the Resource Identification...whether a manuscript contains all the necessary information to be of maximal use to the scientific communities...

  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...directly into the genome to form a stable transfection. Unlike bacterial transformation, scientsts do not "select...Transfection is somewhat comparable to bacterial transformation (the introduction of DNA into bacterial cells...efficient selection. This is usually achieved by performing a "kill curve" (basically growing cells in various...

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...a fusion construct or by co-transfection. HMCES forms a chemical crosslink with DNA abasic sites, protecting... DNA for Cas9 to bind.   R-loop The structure formed by Cas9 binding to DNA and separating the two strands...hypothesized that the adenosine deaminase could also perform cytidine (C) deamination, converting the C base...uracil (U). This is the same chemical reaction performed by CBEs. However, most CBEs include an inhibitor...

  6. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...fluorescent until it binds its protein partner thus forming the full FAP-TAP complex (MG-2I-dL5**).   A FAP-TAP...   Zebrafish experiments with MG-2I-dL5** were performed with embryos and adult fish. Zebrafish are an ...

  7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...exploiting voltage gradients for control of growth and form." Annual review of biomedical engineering 19 (2017...normal genomic background, can even lead to the formation of whole eyes from cell types that don’t typically...

  8. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ... colonies are called satellite colonies and they form when all the antibiotic has been used. Don’t worry...the specific reaction you are running. Transformation Transform a few microliters of your ligation reaction...The PCR could fail to produce a product, the transformation may not result in any cells, or all the colonies...primer to 40-60 bp. It is always a good idea to perform primer optimization, especially if you are having...fragments If you don’t get any positive clone, try transforming the ligation mix in different bacterial strains...

  10. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...product managers collate and organize that information and form it into a roadmap--a plan for future features... needed to know information from the lab folks, it was my job to get it into a format that helped the ...programs, or tools to build. After organizing the information, a software product manager works with software...translate complex lab operations workflows into a format the developers could understand, so they could ... Then, I distill and translate that valuable information back to the rest of my team in Oxford (UK) to...frameworks to use. I mostly sit and listen to stay informed on the details, but the developers have it under...

  11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...“chewed back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable...strong enough to hold a plasmid together through transformation, so the cloned vector must be ligated. But ...repairable by the bacteria after transformation. LIC is a reliable cloning method, but it is limited by its...recombination intermediate to bacteria, so it can be transformed as-is into cells. The cells then get to work ...is long enough to hold it together, it can be transformed into bacteria, which will efficiently repair ... annealed. The recombination intermediate is transformed into bacteria, which repair the plasmid. Created... To overcome this limitation, one option is to perform a hierarchical assembly, assembling fragments in...

  12. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...components of the virus are now delivered in other forms (mRNA, DNA, etc.) to raise immunity. Viral vectors...PMC2075523. Bulcha, J.T., et al. Viral vector platforms within the gene therapy landscape. Sig Transduct...

  13. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...variants of interest, though. A selection can take the form of varied temperature, nutrient availability, drugs...treat cells with many different drugs and then perform an assay to look at an outcome. Your scientific...capacity. Drug screens, on the other hand, are often performed as biased forward screens. A given panel of drugs...reverse screens, except that they begin with a uniform population possessing a specific mutation or phenotype...phenotype. For example, a drug screen may be performed on a cell line possessing a patient mutation to...

  14. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ... focus on practicing with a short and accessible form of writing: alt text (pun intended.)  Steps to clear...present the image and information. Where is the reader accessing the information? This can be physical...readers to provide visually impaired visitors with information about images that a sighted reader would glean...were many barriers between our audience and the information in the paper.  Of course, when you think about...helps visually impaired readers receive the same information as sighted readers.    Figure 1: An image... or digital. For instance, you would present information differently in a poster compared to an online... when writing your alt text, use all of this information to describe the image helpfully. You and your...

  15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...base editors that can be reconstituted in vivo to form a protein identical in sequence to the unmodified...promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and sequences for genomic...plasmids using the Cyanogate Kit and subsequent transformation into cyanobacteria. Image from Vasudevan et...

  17. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...this complex holds a pair of DNA ends together, forming a paired end complex The paired end complex then...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...N-Cas9. The N- and C-Cas9 fragments then dimerize to form a functional protein due to being fused with high-affinity..., dCas9 is released within minutes and red foci formed 20-40 min later. The CASANOVA system is versatile...combinations to optimize both fusion proteins. The best performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65...the catalytic site of AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting...

  19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read on to... of Glycosylation (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals...guanidinium cation (Gdm+) binding in the location formerly occupied by the Arg sidechain. Although not related...

  20. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay (analogous...predict how fluorophores perform in ‘real experiments’, it is advisable to perform time-lapse imaging of ...To determine photostability, an experiment is performed that measures fluorescence intensity over time...Therefore, photostability measurements can be performed with soluble fluorescent proteins or localized...photostability measurements reported in the literature are performed in different ways. We describe a number of issues...used to make these measurements and hope this information can help guide your understanding of reported...fluorescent protein. Photostability measurements can be performed on purified fluorescent proteins. To avoid diffusion...
Showing: 161 - 180 of 759 results