We narrowed to 767 results for: form

...medium, without which the cells cannot reproduce to form colonies (used to complement auxotrophic cell lines...produces pigment from x-gal (included in the transformation plate medium), turning the bacterial colony...for blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies...components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may contain genes that...

...revision before acceptance. F1000Research uses a novel form of peer review that occurs post-publication and ... required information is provided. The clearer you write your paper and the more information you provide...journal’s coverage. These guidelines also provide information on a journal’s house style and the things you...you need to include in your manuscript. What information do I need to include in my paper? More and more...more journals are asking for detailed information on lab resources, such as catalogue numbers and manufacturers...joined a pilot project from the Neuroscience Information Framework (NIF) called the Resource Identification...whether a manuscript contains all the necessary information to be of maximal use to the scientific communities...

...directly into the genome to form a stable transfection. Unlike bacterial transformation, scientsts do not "select...Transfection is somewhat comparable to bacterial transformation (the introduction of DNA into bacterial cells...efficient selection. This is usually achieved by performing a "kill curve" (basically growing cells in various...

...a fusion construct or by co-transfection. HMCES forms a chemical crosslink with DNA abasic sites, protecting... DNA for Cas9 to bind.   R-loop The structure formed by Cas9 binding to DNA and separating the two strands...hypothesized that the adenosine deaminase could also perform cytidine (C) deamination, converting the C base...uracil (U). This is the same chemical reaction performed by CBEs. However, most CBEs include an inhibitor...

...“chewed back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable...strong enough to hold a plasmid together through transformation, so the cloned vector must be ligated. But ...repairable by the bacteria after transformation. LIC is a reliable cloning method, but it is limited by its...recombination intermediate to bacteria, so it can be transformed as-is into cells. The cells then get to work ...is long enough to hold it together, it can be transformed into bacteria, which will efficiently repair ... annealed. The recombination intermediate is transformed into bacteria, which repair the plasmid. Created... To overcome this limitation, one option is to perform a hierarchical assembly, assembling fragments in...

...fluorescent until it binds its protein partner thus forming the full FAP-TAP complex (MG-2I-dL5**).   A FAP-TAP...   Zebrafish experiments with MG-2I-dL5** were performed with embryos and adult fish. Zebrafish are an ...

...exploiting voltage gradients for control of growth and form." Annual review of biomedical engineering 19 (2017...normal genomic background, can even lead to the formation of whole eyes from cell types that don’t typically...

... colonies are called satellite colonies and they form when all the antibiotic has been used. Don’t worry...the specific reaction you are running. Transformation Transform a few microliters of your ligation reaction...The PCR could fail to produce a product, the transformation may not result in any cells, or all the colonies...primer to 40-60 bp. It is always a good idea to perform primer optimization, especially if you are having...fragments If you don’t get any positive clone, try transforming the ligation mix in different bacterial strains...

...components of the virus are now delivered in other forms (mRNA, DNA, etc.) to raise immunity. Viral vectors...PMC2075523. Bulcha, J.T., et al. Viral vector platforms within the gene therapy landscape. Sig Transduct...

...product managers collate and organize that information and form it into a roadmap--a plan for future features... needed to know information from the lab folks, it was my job to get it into a format that helped the ...programs, or tools to build. After organizing the information, a software product manager works with software...translate complex lab operations workflows into a format the developers could understand, so they could ... Then, I distill and translate that valuable information back to the rest of my team in Oxford (UK) to...frameworks to use. I mostly sit and listen to stay informed on the details, but the developers have it under...

...variants of interest, though. A selection can take the form of varied temperature, nutrient availability, drugs...treat cells with many different drugs and then perform an assay to look at an outcome. Your scientific...capacity. Drug screens, on the other hand, are often performed as biased forward screens. A given panel of drugs...reverse screens, except that they begin with a uniform population possessing a specific mutation or phenotype...phenotype. For example, a drug screen may be performed on a cell line possessing a patient mutation to...

...base editors that can be reconstituted in vivo to form a protein identical in sequence to the unmodified...promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and sequences for genomic...plasmids using the Cyanogate Kit and subsequent transformation into cyanobacteria. Image from Vasudevan et...

... focus on practicing with a short and accessible form of writing: alt text (pun intended.)  Steps to clear...present the image and information. Where is the reader accessing the information? This can be physical...readers to provide visually impaired visitors with information about images that a sighted reader would glean...were many barriers between our audience and the information in the paper.  Of course, when you think about...helps visually impaired readers receive the same information as sighted readers.    Figure 1: An image... or digital. For instance, you would present information differently in a poster compared to an online... when writing your alt text, use all of this information to describe the image helpfully. You and your...

...Once expressed, LacZ𝛼 complexes with LacZ𝜔 and forms an active LacZ tetramer.  This tetramer cleaves ...experiments. Potential experiments that one might perform using the purified domains include protein stability...

...polycistronic glycine tRNA-gRNA genes (PTGs) in the forms tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer...

...this complex holds a pair of DNA ends together, forming a paired end complex The paired end complex then...

...N-Cas9. The N- and C-Cas9 fragments then dimerize to form a functional protein due to being fused with high-affinity..., dCas9 is released within minutes and red foci formed 20-40 min later. The CASANOVA system is versatile...combinations to optimize both fusion proteins. The best performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65...the catalytic site of AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting...

...regular building-blocks of living cells, rapidly forming covalent bonds to specific partners under cell-...define what features would allow for the best performance in the most contexts (Figure 1). Together the...the features with optimal biological performance are described as Click Biology (Howarth, 2025). ...SpyCatcher003 SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded peptide (SpyTag)...SnoopCatcher SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded peptide (SnoopTag...DogCatcher SpyTag/SpyCatcher Rapid covalent bond formation between a genetically encoded peptide (DogTag)...tools are already deposited at Addgene in multiple formats: for use in different organisms, targeted to particular...

...good secondaries for cases where you need to pre-form primary-secondary antibody complexes before applying...determines what secondary antibody you should use when performing indirect immunoassays. The Fab regions, on the... is fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make...applying them to samples, such as when performing IHC in a sample where your secondary antibody recognizes...considerations will help you narrow down which antibody format to use for your specific scenario.    References...

...in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay (analogous...predict how fluorophores perform in ‘real experiments’, it is advisable to perform time-lapse imaging of ...To determine photostability, an experiment is performed that measures fluorescence intensity over time...Therefore, photostability measurements can be performed with soluble fluorescent proteins or localized...photostability measurements reported in the literature are performed in different ways. We describe a number of issues...used to make these measurements and hope this information can help guide your understanding of reported...fluorescent protein. Photostability measurements can be performed on purified fluorescent proteins. To avoid diffusion...