We narrowed to 216 results for: gnal

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...bright, exhibits good localization, has high signal:noise ratio, high sensitivity, responds quickly, is...

2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

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   Jan. 31, 2019, 1:32 p.m.

   ...increased expression of transforming growth factor-β signaling, possibly inducing the secretion of growth promoting...

3. Plasmid Tools for Microbiome Studies

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   Blog Post

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   June 27, 2019, 12:25 p.m.

   ...translation of the reporter gene resulting in GFP signal. With the addition of standards, the assay is semi-quantitative...

4. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

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   Dec. 8, 2022, 2:15 p.m.

   ...particularly useful in generating transient edits of signaling molecules (RNA) as opposed to permanent edits ...

5. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   Blog Post

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   April 13, 2017, 2:30 p.m.

   ...targeted to the cytosol when expressed without a signal sequence and to the mitochondria via citrate synthase...

6. CRISPRainbow and Genome Visualization

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   Blog Post

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   Feb. 28, 2017, 3:30 p.m.

   ...dynamics throughout the cell cycle. To optimize the signal to noise ratio of the system, they modified GFP-dCas9...

7. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   Blog Post

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   April 22, 2015, 2:06 p.m.

   ...circuits with many guide RNAs connected because the signal degrades at each layer. If we could fix these things...

8. Hot Plasmids February 2024

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   Blog Post

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   Feb. 20, 2024, 2:45 p.m.

   ... to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing...

9. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   Blog Post

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   Aug. 24, 2021, 1:15 p.m.

   ...primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can bind to a ...

10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post

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    Oct. 6, 2020, 1:15 p.m.

    ...JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence...

11. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

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    June 27, 2017, 1:03 p.m.

    ...small molecules, detect cellular processes and signaling cascades, quantify mechanical tension (a molecular...

12. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

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    April 6, 2017, 1:02 p.m.

    ... function and result in a ubiquitous background signal that makes it hard to visualize the precise location...

13. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    Blog Post

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    Sept. 13, 2016, 2:30 p.m.

    ...outspoken in their opposition to unions, potentially signaling a rough road ahead for students interested in ...

14. The AAVantages of AAV in CRISPR Screens

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    Blog Post

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    Feb. 4, 2025, 2:15 p.m.

    ...delivery vector for gene therapy of human diseases. Signal Transduction and Targeted Therapy, 9(1). https:...

15. Magnetic Control of Proteins: More than a Dream

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    Blog Post

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    Sept. 24, 2024, 1:15 p.m.

    ...expressed and purified. Although a ~1% change in signal might not make a very powerful tool, it was promising...

16. Antibodies 101: Flow Compensation

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    Blog Post

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    May 16, 2024, 1:15 p.m.

    ...telling your cytometer to adjust the fluorescent signals by a specific value between the two channels. It...

17. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

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    May 18, 2021, 1:15 p.m.

    ...enriched in the pre-synaptic terminal providing better signal to noise for imaging calcium dynamics in fine structures...

18. Plasmids 101: Protein Expression

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    Blog Post

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    June 7, 2018, 1:17 p.m.

    ...University and is particularly interested in cell signaling and communication. She loves being able to help...

19. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification RANbodies: Reporter Nanobody...

20. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ... Baltimore. She is particularly interested in signaling peptide maturation in neural and endocrine cells...