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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 250 results for: hal.2

Showing: 161 - 180 of 250 results

  1. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2;8(12):e82009. doi: ...problem that he considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower...

  2. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input concentration across ...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...

  3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...higher quenching of mScarlet emission. Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive...each half with PhyB or PIF6, phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In ...identical in sequence to the unmodified enzyme. Each half of the base editor is fused to a fast-splicing split...induces PhyBΔ and PIF6APB binding, bringing the two halves of Cre together. Image from Eisenhoffer et al.,...

  4. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...Agrobacterium infection. At approximately 7 kb, pSiM24 is 2 and 6 kb smaller than commercially available pCAMBIA...paper at Addgene. Other relevant blog posts 3 Challenges in Plant Synthetic Biology Quick, Versatile Plant...

  5. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...E. coli: acoustic gene 1 (arg1) and acoustic gene 2 (arg2). The key difference between arg1 and arg2 vesicles...widely available imaging platform: ultrasound. Challenges of in vivo imaging with existing bacterial reporters...

  6. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...still depolarize neurons at lower MOIs. Figure 2: ChRgers are delivered by intravenous injection into...optogenetics.  Challenges of engineering channelrhodopsins There are three main challenges to engineering...overcome channelrhodopsin design challenges To overcome these challenges, the Arnold and Gradinaru Labs ...lower multiplicity of infection (MOI) makes it challenging for ChR2(H134R) to function because it’s lower...

  7. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...retroviral library was used to transduce T helper type 2 (Th2) cells from mice that constitutively express ...these indicators difficult to work with.  The Gottschalk lab has surveyed a range of microbial rhodopsin...previously reported for other organisms, and the Gottschalk group hypothesizes that this may be due to different...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...backbone, which expresses the gRNA from a Drosophila U6:2 promoter and Cas9 from the actin 5C promoter. Addgene...backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used GMAP assembly...engineering (MAGE) (1). MAGE uses recombineering (2) to simultaneously incorporate multiple single-strand...number of tandemly expressed gRNAs respectively) and 2) the size of the deletion. Smaller deletions generally...quantification of enhancer activity have remained challenging due to the variable availability of enhancer ...pTALYM3, pTALYM4 Boris Greber pTAL7a, pTAL7b Michal Zurovec pBlue-TAL Nathan Lawson pJDS Series ...degredation is the speed at which it works--typical half lives of AID-tagged proteins after the introduction...

  9. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...(S) labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling...titration of the AAV. So far, tools to overcome this challenge in Drosophila require heat-shocking the system...

  11. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...the world’s population, we account for an estimated 2% of global plastic generation. Less than 9% of plastics...Labconscious. If you’re inspired by this post then I challenge you to take to action! These days, it’s important...

  12. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the drug, the alpha and...constructs, localization of the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering...

  13. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...but also something about how science really works. 2)   Read science writing in magazines or newspapers...   Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding...

  14. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... temporal activation of a transgene. Figure 2: FLEX Cre-Lox system.  WPRE The Woodchuck Hepatitis...sequencing conditions which can read through this challenging sequence. Transgene The transgene is the gene...

  15. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...majority of deposited materials to study SARS-CoV-2 would be fully open and available to industry requestors...difficult time. One function that rose to the challenge was our Finance Team. With Addgene closed for ...

  16. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...make it easier to study these mutants.   Fig. 2: (A) Betacyanin is produced from tyrosine by the activity... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...

  17. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ... sets Taking the time to identify and research 2 – 3 potential professions will highlight which skills.... No brainer! Engaging audiences from across the hall, fields, and other professions increases your chances...

  18. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...particularly new. Chapter 2, “The Professional Scientist,” addresses the challenges of succeeding in science...funding, bioethics in the age of CRISPR, and the challenges of science becoming increasingly politicized....ramifications. Readers unfamiliar with academia’s challenges with publication and funding may be dismayed ...The three chapters in this section describe the challenges of trusting science in a “fake news and post-...describes the author’s thoughts on science’s biggest challenges over the coming years. It is arguably the most...the book? It doesn’t need to. The problems and challenges described in The State of Science have not changed...may be the introduction they need to grasp the challenges you face in your career.  Acknowledgements: ...

  19. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life Sometimes, ... making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are happy at ...and with attention to those around you #4 Thou shalt not bear the burden alone You, alone, are not solely...absolutely equally, which means you only have one-half of the guilt. You can handle that, right? Big tip...get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy...yourself; you will make progress over time. #6 Thou shalt throw money at the problem If you can, pay someone...to relax and be with friends and family. #7 Thou shalt create community to help Involve family. If you ...

  20. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ... Cure for HIV/AIDS. Int J Mol Sci. 2023 Jan 13;24(2):1563. doi: 10.3390/ijms24021563 Resources on Addgene.org...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ... enzymes target several strains of E. coli and lethally chop their genomes into many, many pieces. Locus...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...
Showing: 161 - 180 of 250 results